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1.
Noriaki Murakami Yoshiyuki Tanaka Kunio Takishima Yuzo Minobe Makoto Matsuoka Sei-Ichiro Kiyota Shin-ichi Hatanaka Katsuhiro Sakano 《Journal of plant research》1990,103(4):419-434
We isolated the small subunit of ribulose-1, 5-bisphosphate carboxylase/oxygenase (RuBisCO SSu) from a fern,Asplenium cataractarum and determined its 34 N-terminal amino acid sequence. We obtained a cDNA clone that contains the entire coding region of
the SSu from the same fern species, using synthetic oligonucleotide probes derived from the above amino acid sequence. It
contains a 525 bp open reading frame capable of coding for a polypeptide with 174 amino acids, 31 bp 5′-and 206 bp 3′-noncoding
regions. It was also elucidated that the precursor to the SSu contains a transit peptide of 53 amino acid residues and a mature
protein of 121 residues. We compared the deduced amino acid sequence of the fern SSu with those of 11 other vascular plant
species (including gymnosperms, monocots and dicots). As low as 55% homology was observed between those of a fern and seed
plants. Constancy of the amino acid substitution rate in RuBisCO SSu was supported by our relative rate test. Amino acid substitution
rate per year per site for RuBisCO SSu was calculated to be 0.81×10−9 assuming that the separation between pteridophytes and seed plants arose 380 million years ago. 相似文献
2.
Maki Maekawa Katsuo Fujiwara Naoe Kiyota Chie Yaguchi 《Journal of physiological anthropology》2013,32(1):24
Background
Adaptation changes in postural control and contingent negative variation (CNV) for the elderly were investigated during repeated forward floor translation.Methods
Fifteen healthy elderly persons, living in the suburban area of Kanazawa City, Japan, underwent backward postural disturbance by a forward-floor translation (S2) 2 s after an auditory warning signal (S1). A set with 20 trials was repeated until a negative peak of late CNV was recognized in the 600-ms period before S2, and the last set was defined as the final set. Electroencephalograms, center of foot pressure in the anteroposterior direction (CoPap), and electromyograms of postural muscles were analyzed.Results
CoPap displacement generated by the floor translation was significantly decreased until the twelfth trial in the first set, and mean CoPap displacement was smaller in the second and final sets than in the first set. The mean displacement was significantly smaller in the final set than the previous set. A late CNV with a negative peak was not recognized in the first and second sets. However, most subjects (13/15) showed a negative peak by the fourth set, when the late CNV started to increase negatively from about 1,000 ms after S1 and peaked at about 300 ms before S2. At about 160 ms before the CNV peak, the CoPap forward shift started. The increase in timing of the gastrocnemius activity related to the CoPap shift was significantly correlated with the CNV peak timing (r = 0.64). After S2, peak amplitudes of the anterior postural muscles were significantly decreased in the final set compared to the first set.Conclusions
It was demonstrated that even for the elderly, with so many repetitions of postural disturbance, a late CNV with a negative peak was recognized, leading to accurate postural preparation. This suggests the improvement of frontal lobe function (e.g., anticipatory attention and motor preparation) in the elderly. 相似文献3.
A new taxoid metabolite with a new substitution pattern was isolated from the methanol extract of the seeds of Taxus mairei, and its structure was established as 5alpha,15-dihydroxy-7beta,9alpha-diacetoxy-11(15-->1)abeo-taxa-4(20),11-dien-13-one (1) on the basis of spectral analysis including (1)H- and (13)C-NMR, HMQC, HMBC, NOESY, and confirmed by HR-FAB mass spectrometry. 相似文献
4.
Morphological behavior of acidic and neutral liposomes induced by basic amphiphilic alpha-helical peptides with systematically varied hydrophobic-hydrophilic balance 下载免费PDF全文
Kitamura A Kiyota T Tomohiro M Umeda A Lee S Inoue T Sugihara G 《Biophysical journal》1999,76(3):1457-1468
Lipid-peptide interaction has been investigated using cationic amphiphilic alpha-helical peptides and systematically varying their hydrophobic-hydrophilic balance (HHB). The influence of the peptides on neutral and acidic liposomes was examined by 1) Trp fluorescence quenched by brominated phospholipid, 2) membrane-clearing ability, 3) size determination of liposomes by dynamic light scattering, 4) morphological observation by electron microscopy, and 5) ability to form planar lipid bilayers from channels. The peptides examined consist of hydrophobic Leu and hydrophilic Lys residues with ratios 13:5, 11:7, 9:9, 7:11, and 5:13 (abbreviated as Hels 13-5, 11-7, 9-9, 7-11, and 5-13, respectively; Kiyota, T., S. Lee, and G. Sugihara. 1996. Biochemistry. 35:13196-13204). The most hydrophobic peptide (Hel 13-5) induced a twisted ribbon-like fibril structure for egg PC liposomes. In a 3/1 (egg PC/egg PG) lipid mixture, Hel 13-5 addition caused fusion of the liposomes. Hel 13-5 formed ion channels in neutral lipid bilayer (egg PE/egg PC = 7/3) at low peptide concentrations, but not in an acidic bilayer (egg PE/brain PS = 7/3). The peptides with hydrophobicity less than Hel 13-5 (Hels 11-7 and Hel 9-9) were able to partially immerse their hydrophobic part of the amphiphilic helix in lipid bilayers and fragment liposome to small bicelles or micelles, and then the bicelles aggregated to form a larger assembly. Peptides Hel 11-7 and Hel 9-9 each formed strong ion channels. Peptides (Hel 7-11 and Hel 5-13) with a more hydrophilic HHB interacted with an acidic lipid bilayer by charge interaction, in which the former immerses the hydrophobic part in lipid bilayer, and the latter did not immerse, and formed large assemblies by aggregation of original liposomes. The present study clearly showed that hydrophobic-hydrophilic balance of a peptide is a crucial factor in understanding lipid-peptide interactions. 相似文献
5.
Shi QW Li ZP Zhao D Gu JS Oritani T Kiyota H 《Bioscience, biotechnology, and biochemistry》2004,68(7):1584-1587
Two new taxoid metabolites, 2alpha,7beta,10beta-triacetoxy-5alpha,13alpha-dihydroxy-2(3-->20)abeotaxa-4(20),11-dien-9-one (1) and 2alpha-acetoxy-5alpha-cinnamoyloxy-9alpha,10beta-dihydroxy-3,11-cyclotax-4(20)-en-13-one (2), were isolated from the methanol extract of needles of the Japanese yew, Taxus cuspidata. 相似文献
6.
Ellerby HM Lee S Ellerby LM Chen S Kiyota T del Rio G Sugihara G Sun Y Bredesen DE Arap W Pasqualini R 《The Journal of biological chemistry》2003,278(37):35311-35316
Protein engineering is an emerging area that has expanded our understanding of protein folding and laid the groundwork for the creation of unprecedented structures with unique functions. We previously designed the first native-like pore-forming protein, small globular protein (SGP). We show here that this artificially engineered protein has membrane-disrupting properties and anti-tumor activity in several cancer animal models. We propose and validate a mechanism for the selectivity of SGP toward cell membranes in tumors. SGP is the prototype for a new class of artificial proteins designed for therapeutic applications. 相似文献
7.
Masahiko?HirataEmail author Mihoko?Nakagawa Harumi?Funakoshi Takuya?Iwamoto Waka?Otozu Daisuke?Kiyota Shirou?Kuroki Kiichi?Fukuyama 《Journal of Ethology》2003,21(2):161-168
Distance between dam and offspring (1–121 days old) in a herd of Japanese Black cattle (Bos taurus) grazing a tropical grass (Paspalum notatum) pasture (1.5 ha) was investigated during 7-h grazing periods over grazing seasons from May (spring) to October (autumn).
The mother–young distance was not constant throughout the grazing period, repeatedly increasing and decreasing. Although significant
periodicity was always detected in the mother–young distance, there was no consistent dominant cycle, indicating the complexity
of the within-day pattern of mother–young distance. The mean mother–young distance over the grazing period increased as a
calf aged, reaching a plateau at an age of about 33 days. The mean distance of a calf from its mother was usually shorter
than that from a non-mother cow, with the difference between the mean distances decreasing sharply until a calf became about
35 days old. The results and literature show that mutual independence of mother and young rapidly develops in the first 30–50 days
after parturition.
Electronic Publication 相似文献
8.
We have previously shown that inorganic orthophosphate (Pi)uptake by Catharanthus roseus cells proceeds by a proton/Picotransport mechanism [Sakano (1990) Plant Physiol. 93: 479]that acidifies the cytoplasm [Sakano et al. (1992) Plant Physiol.99: 672]. In the present study, we analyzed changes in the contentof endogenous organic acids, carbon dioxide evolution, and oxygenconsumption upon Pi application. The results are consistentwith the operation of the biochemical pH-stat mechanism [Davies(1986) Physiol. Plant. 67: 702] during and after Pi uptake. (Received November 13, 1997; Accepted March 30, 1998) 相似文献
9.
Chun Fang Shen Taira Kiyota Barbara Jardin Yasuo Konishi Amine Kamen 《Cytotechnology》2007,54(1):25-34
Yeastolate is effective in promoting growth of insect cell and enhancing production of recombinant protein, thus it is a key
component in formulating serum-free medium for insect cell culture. However, yeastolate is a complex mixture and identification
of the constituents responsible for cell growth promotion has not yet been achieved. This study used sequential ethanol precipitation
to fractionate yeastolate ultrafiltrate (YUF) into six fractions (F1–F6). Fractions were characterized and evaluated for their
growth promoting activities. Fraction F1 was obtained by 65% ethanol precipitation. When supplemented to IPL-41 medium at
a concentration of 1 g L−1, fraction F1 showed 71% Sf-9 cell growth improvement and 22% β-galactosidase production enhancement over YUF (at 1 g L−1 in IPL-41 medium). However, the superiority of F1 over YUF on promoting cell growth gradually diminished as its concentration
in IPL-41 medium increased. At 4 g L−1, the relative activity of F1 was 93% whereas YUF was 100% at the same concentration. At 1 g L−1, four other fractions (F2–F5) precipitated with higher ethanol concentrations and F6, the final supernatant, showed growth
promoting activities ranging from 32 to 80% as compared to YUF (100%). Interestingly, a synergistic effect on promoting cell
growth was observed when F6 was supplemented in IPL-41 medium in presence of high concentrations of F1 (>3 g L−1). The results suggest that ethanol precipitation was a practical method to fractionate growth-promoting components from YUF,
but more than one components contributed to the optimum growth of Sf-9 cells. Further fractionation, isolation and identification
of individual active components would be needed to better understand the role of these components on the cell metabolism. 相似文献
10.
Dong M Zhang ML Wang YF Zhang XP Li CF Shi QW Cong B Kiyota H 《Bioscience, biotechnology, and biochemistry》2007,71(8):2087-2090
Three novel taxanes with the 10-alkoxy group were isolated from heartwood of Taxus cuspidata. The structures were identified as 2alpha,14beta-diacetoxy-10beta-ethoxytaxa-11,4(20)-dien-5alpha-ol (1), 2alpha,14beta-diacetoxy-10beta-methoxytaxa-11,4(20)-dien-5alpha-ol (2), and 2alpha-acetoxy-10beta-ethoxytaxa-11,4(20)-diene-5alpha,14beta-diol (3) on the basis of spectroscopic analysis. These are the first taxanes with an alkoxy moiety on the skeleton. 相似文献