全文获取类型
收费全文 | 6999篇 |
免费 | 755篇 |
出版年
2023年 | 37篇 |
2022年 | 88篇 |
2021年 | 187篇 |
2020年 | 101篇 |
2019年 | 115篇 |
2018年 | 144篇 |
2017年 | 137篇 |
2016年 | 206篇 |
2015年 | 348篇 |
2014年 | 403篇 |
2013年 | 415篇 |
2012年 | 533篇 |
2011年 | 495篇 |
2010年 | 319篇 |
2009年 | 264篇 |
2008年 | 411篇 |
2007年 | 366篇 |
2006年 | 350篇 |
2005年 | 307篇 |
2004年 | 310篇 |
2003年 | 252篇 |
2002年 | 283篇 |
2001年 | 117篇 |
2000年 | 99篇 |
1999年 | 93篇 |
1998年 | 66篇 |
1997年 | 55篇 |
1996年 | 50篇 |
1995年 | 36篇 |
1994年 | 38篇 |
1993年 | 46篇 |
1992年 | 54篇 |
1991年 | 57篇 |
1990年 | 53篇 |
1989年 | 57篇 |
1988年 | 44篇 |
1987年 | 36篇 |
1986年 | 46篇 |
1985年 | 41篇 |
1983年 | 30篇 |
1982年 | 32篇 |
1980年 | 30篇 |
1979年 | 35篇 |
1978年 | 29篇 |
1976年 | 26篇 |
1975年 | 29篇 |
1974年 | 37篇 |
1973年 | 31篇 |
1971年 | 27篇 |
1968年 | 26篇 |
排序方式: 共有7754条查询结果,搜索用时 248 毫秒
1.
2.
3.
4.
Sign language studies of cross-fostered chimpanzees measure the effect of special rearing conditions on the development of
very young chimpanzees. Cross-fostered chimpanzees, like human children, develop gradually in a process that takes many years.
Here we discuss details of the procedure, the overlap between human and chimpanzee infants in the contents of the first 50-item
vocabularies, and the ways in which the signs of the chimpanzees exhibit the fuzziness of natural language categories. We
also compare the cross-fostering approach with more traditional modular approaches to the study of language-like behavior
in nonhuman animals.
Project Washoe was originally supported by grants MH-12154 from the National Institute of Mental Health and GB-7432 from the
National Science Foundation. We gratefully acknowledge this support and the support that later sign language studies of chimpanzees
have received since then from NIH, NSF, the National Geographic Society, the Grant Foundation, the Spencer Foundation, the
University of Nevada, and the UNR Foundation. 相似文献
5.
Daneen Schaeffer Filipa Pereira Reis Sean J. Johnson Cec��lia Maria Arraiano Ambro van Hoof 《Nucleic acids research》2012,40(18):9298-9307
The 10-subunit RNA exosome is involved in a large number of diverse RNA processing and degradation events in eukaryotes. These reactions are carried out by the single catalytic subunit, Rrp44p/Dis3p, which is composed of three parts that are conserved throughout eukaryotes. The exosome is named for the 3′ to 5′ exoribonuclease activity provided by a large C-terminal region of the Rrp44p subunit that resembles other exoribonucleases. Rrp44p also contains an endoribonuclease domain. Finally, the very N-terminus of Rrp44p contains three Cys residues (CR3 motif) that are conserved in many eukaryotes but have no known function. These three conserved Cys residues cluster with a previously unrecognized conserved His residue in what resembles a metal-ion-binding site. Genetic and biochemical data show that this CR3 motif affects both endo- and exonuclease activity in vivo and both the nuclear and cytoplasmic exosome, as well as the ability of Rrp44p to associate with the other exosome subunits. These data provide the first direct evidence that the exosome-Rrp44p interaction is functionally important and also provides a molecular explanation for the functional defects when the conserved Cys residues are mutated. 相似文献
6.
A method for identifying cysteine-containing peptides in proteins is presented using 2-bromoacetamido-4-nitrophenol (BNP) to introduce an easily detectable probe. The formation of a covalent bond between the protein sulfhydryl group and the acetamido moiety of BNP introduces a chromophore with an absorbance maximum at 410 nm. The modified protein can then be cleaved with appropriate proteases and the resulting peptides separated by chromatographic methods. Monitoring the effluent at a single wavelength (405 nm) provides a rapid and simple method of detecting and isolating only those peptides which contain cysteine residue(s). The nitrophenol derivative is stable under conditions required for protease cleavage. The reagent is therefore useful for locating cysteine-containing peptides in protein digests and can be used to explore the accessibility of different cysteines under a variety of conditions. The ease of modification, specificity of reaction, product stability, and simple detection of modified peptides make BNP ideal for investigation of cysteine residues. 相似文献
7.
Eric S. J. Harris Shugeng Cao Sean D. Schoville Chengming Dong Wenquan Wang Zaiyou Jian Zhongzhen Zhao David M. Eisenberg Jon Clardy 《PloS one》2012,7(11)
Oridonin is a diterpenoid with anti-cancer activity that occurs in the Chinese medicinal plant Isodon rubescens and some related species. While the bioactivity of oridonin has been well studied, the extent of natural variation in the production of this compound is poorly known. This study characterizes natural variation in oridonin production in order to guide selection of populations of Isodon with highest oridonin yield. Different populations of I. rubescens and related species were collected in China, and their offspring were grown in a greenhouse. Samples were examined for oridonin content, genotyped using 11 microsatellites, and representatives were sequenced for three phylogenetic markers (ITS, rps16, trnL-trnF). Oridonin production was mapped on a molecular phylogeny of the genus Isodon using samples from each population as well as previously published Genbank sequences. Oridonin has been reported in 12 out of 74 species of Isodon examined for diterpenoids, and the phylogeny indicates that oridonin production has arisen at least three times in the genus. Oridonin production was surprisingly consistent between wild-collected parents and greenhouse-grown offspring, despite evidence of gene flow between oridonin-producing and non-producing populations of Isodon. Additionally, microsatellite genetic distance between individuals was significantly correlated with chemical distance in both parents and offspring. Neither heritability nor correlation with genetic distance were significant when the comparison was restricted to only populations of I. rubescens, but this result should be corroborated using additional samples. Based on these results, future screening of Isodon populations for oridonin yield should initially prioritize a broad survey of all species known to produce oridonin, rather than focusing on multiple populations of one species, such as I. rubescens. Of the samples examined here, I. rubescens or I. japonicus from Henan province would provide the best source of oridonin. 相似文献
8.
9.
A neonate with aniridia was found to have a one band paracentric inversion of the short arm of chromosome 4. This was initially difficult to interpret on high resolution banding. The inversion was present in three generations of the family. 相似文献
10.
Plants often suffer reductions in fecundity due to insect herbivory. Whether this loss of seeds has population-level consequences
is much debated and often unknown. For many plants, particularly those with long-lived seedbanks, it is frequently asserted
that herbivores have minimal impacts on plant abundance because safe-site availability rather than absolute seed number determines
the magnitude of future plant recruitment and hence population abundance. However, empirical tests of this assertion are generally
lacking and the interplay between herbivory, spatio-temporal variability in seed- or safe-site-limited recruitment, and seedbank
dynamics is likely to be complex. Here we use a stochastic simulation model to explore how changes in the spatial and temporal
frequency of seed-limited recruitment, the strength of density-dependent seedling survival, and longevity of seeds in the
soil influence the population response to herbivory. Model output reveals several surprising results. First, given a seedbank,
herbivores can have substantial effects on mean population abundance even if recruitment is primarily safe-site-limited in
either time or space. Second, increasing seedbank longevity increases the population effects of herbivory, because annual
reductions in seed input due to herbivory are accumulated in the seedbank. Third, population impacts of herbivory are robust
even in the face of moderately strong density-dependent seedling mortality. These results imply that the conditions under
which herbivores influence plant population dynamics may be more widespread than heretofore expected. Experiments are now
needed to test these predictions.
Received: 3 November 1999 / Accepted: 15 February 2000 相似文献