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1.
Richard D. Howard Howard H. Whiteman Teresa I. Schueller 《Evolution; international journal of organic evolution》1994,48(4):1286-1300
Adaptive mate choice in species lacking male resource control and/or paternal care might be maintained by selection because preferred males sire genetically superior offspring. For such a process to occur, some male phenotypic trait(s) must both reliably indicate male genetic quality and influence the pattern of mate choice by females. In American toads, Bufo americanus, male body length has been documented to influence female mating patterns: females usually mate with males that are larger than average. However, the relationship between male size and male genetic quality is unknown. We conducted a controlled breeding experiment using 48 sires and 19 dams to determine if larger males sire offspring with superior larval performance characteristics (greater survival to metamorphosis, larger mass at metamorphosis, and earlier metamorphosis). We also aged each sire to test the hypothesis that older males are, on average, genetically superior to younger males. We crossed each female with three sires representing three body size categories (mean and 1 SD ± mean snout-ischium length). Hatchlings (500 from each cross) were reared to metamorphosis in seminatural ponds in the field. Metamorph weight (log transformed) and age at metamorphosis showed significant heritability and were genetically correlated with each other. Hence, sires differed in genetic quality. However, none of the three measures of offspring performance was correlated with sire body size or age. Thus, we obtained no support for the prediction that sire body size or age is related to genetic quality. 相似文献
2.
Song Lihui Agtuca Beverly Schueller Michael J. Jurisson Silvia S. Stacey Gary Ferrieri Richard A. 《Journal of Plant Growth Regulation》2019,38(1):164-179
Journal of Plant Growth Regulation - Experiments designed to quantify the physiological and metabolic status of Arabidopsis thaliana seedlings across their photoperiod used wild-type (Col-0) and... 相似文献
3.
Terryn N Heijnen L De Keyser A Van Asseldonck M De Clercq R Verbakel H Gielen J Zabeau M Villarroel R Jesse T Neyt P Hogers R Van Den Daele H Ardiles W Schueller C Mayer K Déhais P Rombauts S Van Montagu M Rouzé P Vos P 《FEBS letters》1999,445(2-3):237-245
As part of the European Scientists Sequencing Arabidopsis program, a contiguous region (396607 bp) located on chromosome 4 around the APETALA2 gene was sequenced. Analysis of the sequence and comparison to public databases predicts 103 genes in this area, which represents a gene density of one gene per 3.85 kb. Almost half of the genes show no significant homology to known database entries. In addition, the first 45 kb of the contig, which covers 11 genes, is similar to a region on chromosome 2, as far as coding sequences are concerned. This observation indicates that ancient duplications of large pieces of DNA have occurred in Arabidopsis. 相似文献
4.
Sheila K. Schueller 《Evolutionary ecology》2007,21(1):81-98
A match between floral and pollinator traits, such as that between unique island plants and pollinators, is often thought
to be the product of pollinator-mediated selection. I examined whether the floral morphology of an introduced hummingbird-pollinated
plant, Nicotiana glauca (tree tobacco, Solanaceae), is under selection by pollinators on the California Channel Islands where it is a recent colonist.
I first determined differences in floral morphology and pollinator composition between island and mainland populations of
N. glauca. I found that island plants have detectably longer corollas (on average 1 mm) and are visited by hummingbird species with
on average 1–2 mm longer bills than common mainland visitors. Corolla length differences were not found to be associated with
site abiotic differences. Flower size does not vary consistently with season and corolla width is very consistent across sites.
I tested whether island–mainland corolla length differences are the product of pollinator-mediated selection by measuring
phenotypic selection and per visit effectiveness. Contrary to expectations, a longer corolla was not consistently associated
with higher pollen transfer or seed count on the islands. Per visit effectiveness of longer and shorter-billed hummingbirds
did differ; however, effectiveness did not depend on corolla length. Although I failed to detect expected patterns of selection
for longer corollas on islands, I cannot rule out weak or past pollinator-mediated selection. It is also possible that despite
the apparent match between pollinator and floral traits, island–mainland differences in corolla length are instead due to
other environmental effects, selection unrelated to pollinators, or stochastic processes such as drift. 相似文献
5.
Schueller C Schneider B Kempf VA Haas A 《Microbes and infection / Institut Pasteur》2007,9(3):355-363
Afipia felis is a Gram-negative alpha-proteobacterium, a rare cause of human cat scratch disease (CSD), and likely a pathogen of amoeba. Here, we show that various members of the genus Afipia attach to and are taken up by various non-professional phagocytic mammalian cells (epithelial CHO, endothelial EA.hy926, epithelial HeLa, epithelial INT407 cells, endothelial HMEC-1, endothelial HUVEC, and fibroblast L929 cells). However, only A. felis was able to do this efficiently. Invasion depended on a functional actin cytoskeleton and much less on microtubule dynamics. Bacteria were slowly taken up into HMEC-1 (and HUVEC) via pocket-like structures and they resided within membrane-surrounded phagosomes. While A. felis was found in a non-canonical endocytic compartment in macrophage cells, Afipia-containing phagosomes in HMEC-1 were transiently positive for early endosomal EEA1 and then became and remained positive for lysosome-associated membrane protein-1 (LAMP1) and the proton-pumping ATPase, suggesting undisturbed, albeit slowed, phagosome biogenesis in these cells. Similarly, at 24h of infection, most phagosomes in HeLa, INT407, HUVEC and in EA.hy926 cells were positive for LAMP1. In summary, A. felis enters various non-professional phagocytes and its compartmentation differs between macrophages and non-professional phagocytes. 相似文献
6.
Tassilo?Gleede Barbara?Riehl Colleen?Shea Lena?Kersting Aylin?Sibel?Cankaya David?Alexoff Michael?Schueller Joanna?S.?Fowler Wenchao?QuEmail author 《Amino acids》2015,47(3):525-533
Carbon-11 (β+ emitter, t 1/2 = 20.4 min) radiolabeled l-glutamine is a potentially useful molecular imaging agent that can be utilized with positron emission tomography for both human oncological diagnosis and plant imaging research. Based upon a previously reported [11C]cyanide end-capping labeling method, a systematic investigation of nucleophilic cyanation reactions and acidic hydrolysis reaction parameters, including base, metal ion source, phase transfer catalyst, solvent, reaction temperature and reaction time, was conducted. The result was a milder, more reliable, two-step method which provides l-[5-11C]-glutamine with a radiochemical yield of 63.8 ± 8.7 % (range from 51 to 74 %, n = 10) with >90 % radiochemical purity and >90 % enantiomeric purity. The total synthesis time was 40–50 min from the end of bombardment. In addition, an Fmoc derivatization method was developed to measure the specific activity of this radiotracer. 相似文献
7.
Vânia C. S. Pankievicz Fernanda P. do Amaral Karina F. D. N. Santos Beverly Agtuca Youwen Xu Michael J. Schueller Ana Carolina M. Arisi Maria. B.R. Steffens Emanuel M. de Souza Fábio O. Pedrosa Gary Stacey Richard A. Ferrieri 《The Plant journal : for cell and molecular biology》2015,81(6):907-919
Nitrogen‐fixing rhizobacteria can promote plant growth; however, it is controversial whether biological nitrogen fixation (BNF) from associative interaction contributes to growth promotion. The roots of Setaria viridis, a model C4 grass, were effectively colonized by bacterial inoculants resulting in a significant enhancement of growth. Nitrogen‐13 tracer studies provided direct evidence for tracer uptake by the host plant and incorporation into protein. Indeed, plants showed robust growth under nitrogen‐limiting conditions when inoculated with an ammonium‐excreting strain of Azospirillum brasilense. 11C‐labeling experiments showed that patterns in central carbon metabolism and resource allocation exhibited by nitrogen‐starved plants were largely reversed by bacterial inoculation, such that they resembled plants grown under nitrogen‐sufficient conditions. Adoption of S. viridis as a model should promote research into the mechanisms of associative nitrogen fixation with the ultimate goal of greater adoption of BNF for sustainable crop production. 相似文献
8.
Schueller SK 《American journal of botany》2004,91(5):672-681
Traits associated with self-pollination are common in island plants. This pattern could simply reflect the vestige of selection during colonization. Alternatively (or in addition), the ability to self-pollinate may provide a reproductive assurance benefit in established island plant populations due to inferior island pollinator service. To test these alternatives I studied an introduced plant (Nicotiana glauca; Solanaceae) on the California mainland and on two Channel Islands colonized at different times (approximately 30 and 100 yr ago). I compared these populations in terms of (1) capacity for self-pollination (self-compatibility, autogamy, stigma-anther distance, and incidence of a crumpled floral morph) and (2) current selection for the ability to self-pollinate (pollinator service by hummingbirds and the effect of emasculation on reproductive success). In general, island plants exhibited a higher capacity for self-pollination than mainland plants, especially on the most recently colonized island. However, island plants were not visited less frequently or more variably, nor did I detect current selection for selfing on islands. This supports the hypothesis that selfing traits in island plants are the product of a filter to successful establishment during colonization and not of selection for selfing in established island populations. 相似文献
9.
Validamycin A was used to inhibit in vivo trehalase activity in tobacco enabling the study of subsequent changes in new C partitioning into cellulosic biomass and lignin precursors. After 12-h exposure to treatment, plants were pulse labeled using radioactive 11CO2, and the partitioning of isotope was traced into [11C]cellulose and [11C]hemicellulose, as well as into [11C]phenylalanine, the precursor for lignin. Over this time course of treatment, new carbon partitioning into hemicellulose and cellulose was increased, while new carbon partitioning into phenylalanine was decreased. This trend was accompanied by a decrease in phenylalanine ammonia-lyase activity. After 4 d of exposure to validamycin A, we also measured leaf protein content and key C and N metabolite pools. Extended treatment increased foliar cellulose and starch content, decreased sucrose, and total amino acid and nitrate content, and had no effect on total protein. 相似文献
10.
We examined the influence of pollen competitive environment on pollen performance in Mirabilis
jalapa. We used the number of pollen grains and the number of pollen tubes per pistil as measures of pollen competition. Pollen
germination, pollen tube penetration into the style, and pollen tube growth rates were used as measures of pollen performance.
All three measures of pollen performance were affected by the competitive environment. Pollen germination was greatest at
intermediate pollen load sizes. The percentage of germinated pollen grains that penetrated the stigma and grew into the style
decreased with pollen load size. Pollen tube growth rate in the style was greater and more variable with larger numbers of
pollen tubes in the style. Controlling for the degree of selection at the stigma indicated that pollen-pollen or pollen-style
interactions were the likely causes of increased growth rates.
Received: 28 October 1996 / Revision accepted: 24 January 1997 相似文献