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Merz Felicitas Müller Mareike Taucher-Scholz Gisela Rödel Franz Stöcker Horst Schopow Kosta Laprell Laura Dehghani Faramarz Durante Marco Bechmann Ingo 《Radiation and environmental biophysics》2010,49(3):457-462
The aim of this interdisciplinary project is to establish slice culture preparations from rodents and humans as a new model
system for studying effects of X-rays and heavy ions within normal and tumor tissues. The advantage of such slice cultures
relies on the conservation of an organotypic environment, the easy treatment and observation by live-imaging microscopy, and
the independence from genetic immortalization strategies used to generate cell lines. Rat brains as well as human tumors were
cut into 300-μm-thick sections and cultivated in an incubator in a humidified atmosphere at 37°C. This is realized by a membrane-based
culture system with a liquid–air interface. With this system, it is possible to keep rodent slices viable for several months.
Human brain tumor slices remained vital for at least 21 days. Slices were irradiated with X-rays at the radiation facility
of the University Hospital in Frankfurt/Main at doses up to 40 Gy. Heavy ion irradiations were performed at GSI (Darmstadt)
with different ions, energies, and doses. The irradiated slices were analyzed by 3D-confocal microscopy following immunostaining
for DNA damage, microglia, and proliferation markers. The phosphorylated histone γH2AX proved to be suitable for the detection
of ion traversals in this system. 相似文献
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Lymphocytes and other blood cells can be separated by means of free flow cell electrophoresis. Immunofluorescence of the separated lymphocytes of four healthy volunteers with antiimmunoglobulins IgD and IgM produced different distribution profiles for each immunoglobulin class, the IgD positive cells migrating faster than the IgM positive ones. Amongst five patients with chronic lymphocytic leukemia four with IgD positive lymphocytes (greater than 80%) showed an identical electrophoretic distribution. The IgM positive lymphocytes (greater than 80%) of the fifth patient migrated much more slowly. The weighted mean of each distribution profile of either the IgD or IgM positive lymphocytes in CLL is similar to that of normal subjects. 相似文献
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