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An improved complement-fixation technique in foot-and-mouth disease is described in details. In the development of the technique consideration has been given to the established presence of an antigen-excessphenomenon as well as to the linear relationship between amounts of immune serum used and complement fixed. The increased accuracy of the technique has made it possible more precise and rapid to detect even small serological differences. 相似文献
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A sandwich type ELISA for foot-and-mouth disease (FMD) virus types O, A and C was established, using a combination of rabbit anti-146 S and guinea pig hyperimmune antibodies. This method was found to be highly efficient for the detection of both 146 S particles and 12 S subunits. The ELISA was approximately 500 times more sensitive than complement fixation (CF) when examining epithelial samples of FMD vesicles. An early primary diagnosis of FMD was obtained by both CF and ELISA in 19 out of 21 confirmed cases. The remaining 2 cases were initially negative in CF but positive in ELISA. 相似文献
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