排序方式: 共有4条查询结果,搜索用时 0 毫秒
1
1.
Recent evidence suggests that several unknown or ill-characterized factors strongly influence cell growth and function in
culture. Isolating these factors is necessary in order to maximize culture productivities. Methylglyoxal (MG), a potent protein
and nucleic acid modifying agent, has been identified as a player in the signaling pathways associated with cell death and
is known to be detrimental to cultured cells. This compound is produced in all mammalian systems by spontaneous phosphate
elimination from glycolytic pathway intermediates. A kinetic model that qualitatively describes the cellular distribution
of protein-associated MG in the absence of enzymatic adduct formation predicted far lower levels of reversibly bound MG than
measured in cultured CHO cells. This suggests that the targeted modification of proteins through enzymatically mediated mechanisms
is a significant sink for cellular methylglyoxal. The model was validated with measurements of carbon flux through the glyoxalase
pathway to d-lactic acid, a unique end product of MG metabolism in mammalian systems. Fluxes to d-lactic acid of up to 16.8 mmol
ml-packed cells−1 day−1 were measured with CHO cells grown in batch culture or 100-fold more than found in normal tissues. 相似文献
2.
Sukanya Jeennor Pattsarun Cheawchanlertfa Sarinya Suttiwattanakul Sarocha Panchanawaporn Chanikul Chutrakul Kobkul Laoteng 《Biochemical and biophysical research communications》2014
We identified a novel elongase gene from a selected strain of the Oomycete, Pythium sp. BCC53698. Using a PCR approach, the cloned gene (PyElo) possessed an open reading frame (ORF) of 834 bp encoding 277 amino acid residues. A similarity search showed that it had homology with the PUFA elongases of several organisms. In addition, the signature characteristics, including four conserved motifs, a histidine-rich catalytic motif and membrane-associated feature were present in the Pythium gene. Heterologous expression in Saccharomyces cerevisiae showed that it was specific for fatty acid substrates, having a double bond at Δ6-position, which included γ-linolenic acid (GLA) and stearidonic acid (STA), and preferentially elongated the n3-18C PUFA. This is an elongase in Oomycete fungi, which displays very high specificity on Δ6-18C desaturated fatty acids. This will be a powerful tool to engineer PUFA biosynthesis in organisms of interest through the n-6 series pathway for producing value-added fatty acids. 相似文献
3.
4.
Mungkung Rattanawan Gheewala Shabbir H. Silalertruksa Thapat Dangsiri Sarocha 《The International Journal of Life Cycle Assessment》2019,24(12):2128-2139
The International Journal of Life Cycle Assessment - Rice is a major economic crop which has created local livelihood, careers and incomes in the agricultural sector in Thailand, one of the leading... 相似文献
1