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排序方式: 共有140条查询结果,搜索用时 15 毫秒
1.
L M Iarovaia E A Shmeleva V S Samsonova E V Kulikovskaia 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1985,(8):26-30
The chemical characteristics of 6 batches of the preparation, obtained from the cell wall of C. diphtheriae grown in liquid and solid culture media, with respect to their content of nitrogen, hexoses, pentoses, total amino sugars, lipids and to the possible admixture of nucleic acids are presented. From the results of the chemical analysis of these batches their standardization according to the ratio of total amino sugars and pentoses to total nitrogen in C. diphtheriae cell-wall preparation is proposed. 相似文献
2.
Heike Schmidt Rüdiger Bode Ida A. Samsonova Dieter Birnbaum 《Applied microbiology and biotechnology》1989,31(5-6):463-466
Summary Mutants of Candida maltosa were isolated that lacked saccharopine reductase (lys9) and saccharopine dehydrogenase (lys1) and were able to accumulate -aminoadipate--semialdehyde (AASA) in the cell and excrete it into the culture medium. The effects of incubation time, lysine concentration, and carbon and nitrogen sources on AASA production were examined. In the presence of 15 g glucose/1, 1.25 g NH4H2PO4/l and 50 mg l-lysine/l in a minimal salt medium C. maltosa G285 (lys1) produced about 80–90 mg AASA/l during 48 h of growth. A simple and rapid procedure to isolate AASA from the medium using Dowex 50X4 is described. 相似文献
3.
The usefulness of hybridization by protoplast fusion and mitotic segregation for the genetic analysis of the imperfect fodder yeastCandida maltosa was tested. Mitotically stable fusion hybrids were obtained with frequencies between 10–6 and 10–7. Complementation tests were performed by protoplast fusion. Substances that are known to induce frequent mitotic segregation in other yeast species such as benomyl, p-fluorophenylalanine, and acriflavine were ineffective inC. maltosa. UV irradiation induced mitotic segregation in up to 10%. This agent induced mainly mitotic crossing over inC. maltosa. Our data enabled the construction of the linkage group I with the sequenceCEN-ade-26-pro-1. 相似文献
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Sokolkova A. B. Bulyntsev S. V. Chang P. L. Carrasquila-Garcia N. Cook D. R. von Wettberg E. Vishnyakova M. A. Nuzhdin S. V. Samsonova M. G. 《Biophysics》2021,66(3):395-400
Biophysics - The concentration of genes that control domestication traits in so-called “agroislands” complicates breeding. Therefore, information about where such islands are located in... 相似文献
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Biophysics - Abstract—A method for certification of soybean varieties was developed based on single-nucleotide polymorphisms. A genetic algorithm was used to select an optimal set consisting... 相似文献
9.
Taratuhin O. D. Novikova L. Yu. Seferova I. V. Gerasimova T. V. Nuzhdin S. V. Samsonova M. G. Kozlov K. N. 《Biophysics》2020,65(1):106-117
Biophysics - Abstract—Soybean phenology is strongly influenced by temperature and day length, and phenological records clearly reflect the changes in climatic conditions. A model including... 相似文献
10.
MOTIVATION: A new method is developed to query a relational database in natural language (NL). RESULTS: The method, based on a semantic approach, interprets grammatical and lexical units of a natural language into concepts of subject domain, which are given in a conceptual scheme. The conceptual scheme is mapped formally onto the logical scheme. We applied the method to query the FlyEx database in natural language. FlyEx contains information on the expression of segmentation genes in Drosophila melanogaster. The method allows formulation of queries in various natural languages simultaneously, and is adaptive to changes in the knowledge domain and user's views. It provides optimal transformation of queries from natural language to SQL, as well as visualization of information as a hyperscheme. The method does not require specification of all possible language constructions as well as a standard grammar accuracy in formulation of NL queries. 相似文献