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The aim of this study was to delineate the flow patterns in a non-unidirectional flow field inside a ventricle-shaped cell
culture chamber, and examine the resulting morphology and integrity of the endothelium in select regions of the monolayer.
The chamber was perfused by pulsatile flow, and the coherent motion of the fluid was studied using flow visualization aided
by image analysis. Four distinct flow patterns were discerned and examined: central jet, flow impingement, flow separation,
and recirculating eddies. The influence of these patterns on endothelial cell morphology was assessed after 20 h of exposure
to flow. There were no signs of damage to the endothelium in the jet region nor was there evidence of cell alignment with
the flow. Yet, there were changes in cell morphology and cytoskeletal architecture as compared to control. By contrast, within
the eddies where the flow was highly disturbed, there was apparent damage to the endothelium. Thus, exposure of cells to random
velocity fluctuations in regions of quasi-static flow compromises the integrity of the monolayer. Identification of such sites
and acquisition of the knowledge necessary to protect the cells from denudation will be valuable for the endothelialization
efforts of cardiac prostheses. 相似文献
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Samet Evyapan Emine Elçin Oruç-Emre Yusuf Sıcak Ayşegül Karaküçük-İyidoğan Gizem Tatar Yılmaz Mehmet Öztürk 《化学与生物多样性》2023,20(8):e202300626
In this study, new chiral thiourea and 1,3-thiazolidine-4,5-dione derivatives were synthesized, it was aimed to evaluate the various biological activities and molecular docking of these compounds. Firstly, the new thioureas ( 1 – 16 ) were obtained by reacting 1-naphthylisothiocyanate with different chiral amines. Then, the chiral thioureas were cyclized with oxalyl chloride to obtain 1,3-thiazolidine-4,5-dione derivatives ( 17 – 32 ). All compounds were evaluated with several in vitro antioxidant and enzyme inhibition activities. Compound 30 was the most active compound against AChE, with a value of IC50=8.09±0.58 μM. On the other hand, all compounds were tested in silico absorption, distribution, metabolism, and excretion (ADME) assays to better understand their bioavailability. These physicochemical properties, pharmacokinetics, and drug-likeness of all compounds were calculated using SwissADME. Furthermore, according to molecular docking analyses compound 30 exhibited significant binding affinities for all enzymes. Based on our overall observations, compound 30 could be recommended as a potential lead for the therapuetic of Alzheimer's. 相似文献
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Havva Balseven M. Mustafa İşgör Samet Mert Zuhal Alım Şükrü Beydemir Salim Ok Rahmi Kasımoğulları 《Bioorganic & medicinal chemistry》2013,21(1):21-27
In the current study, a series of pyrazole-sulfonamide derivatives (2–14) were synthesized, characterized, and the inhibition effects of the derivatives on human carbonic anhydrases (hCA I and hCA II) were investigated as in vitro. Structures of these sulfonamides were confirmed by FT-IR, 1H NMR, 13C NMR and LC–MS analysis. 1H NMR and 13C NMR revealed the tautomeric structures. hCA I and hCA II isozymes were purified from human erythrocytes and inhibitory effects of newly synthesized sulfonamides on esterase activities of these isoenzymes have been studied. The Ki values of compounds were 0.062–1.278 μM for hCA I and 0.012–0.379 μM for hCA II. The inhibition effects of 7 for hCA I and 4 for hCA II isozymes were almost in nanomolar concentration range. 相似文献
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Krawczyk B Naumiuk L Lewandowski K Baraniak A Gniadkowski M Samet A Kur J 《FEMS immunology and medical microbiology》2003,38(3):241-248
Amplification of DNA fragments surrounding rare restriction sites (ADSRRS-fingerprinting) is a novel assay based on suppression of polymerase chain reaction (PCR). This phenomenon allows the amplification of only a limited subset of DNA fragments, since only those with two different oligonucleotides ligated at the ends of complementary DNA strands are amplified in the PCR. The DNA fragments can be easily analyzed on polyacrylamide gels, stained with ethidium bromide. We have implemented this method using a set of clinical Serratia marcescens isolates from three outbreaks ongoing in the Public Hospital in Gdańsk (Poland). Clustering of ADSRRS-fingerprinting data matched epidemiological, microbiological, random amplification of polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) data. Based on this study, we found that there is at least a similar power of discrimination between the present 'gold-standard' PFGE and the novel method, ADSRRS-fingerprinting. Although the ADSRRS-fingerprinting method may appear to be more complex than the RAPD technique, we found it fast and reproducible. 相似文献
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Troudi A Amara IB Samet AM Fetoui H Soudani N Guermazi F Boudawara T Zeghal N 《BioFactors (Oxford, England)》2011,37(6):429-438
Gibberellic acid (GA?) has been worldwide used in agriculture as a plant growth regulator. The purpose of this study is to assess the effects of GA? on the morphology and the thyroid hormone levels in adult rats and their suckling pups. Animals were given daily 200 ppm GA? in drinking water from the 14th day of pregnancy until day 14 after delivery. Compared with a control group, GA?-treated mothers and pups showed an increase in body and thyroid weights, a decrease in plasma FT? and FT? levels, which were more pronounced in pups than in their mothers. Thyroid iodine content was also decreased in pups. These biochemical modifications corresponded histologically; the majority of follicles had cubical epithelial cells, which surrounded empty vesicular cavities. Toxicity was objectified by a significant increase in plasma malondialdehyde, protein carbonyls, and advanced oxidation protein products levels in GA?-treated dams and their suckling pups; while, the activities of superoxide dismutase, catalase, and glutathione peroxidase were decreased in plasma of both dams and their pups. Moreover, a significant decline was observed in plasma glutathione, nonprotein thiols, and vitamin C levels. We conclude that GA? treatment affects thyroid function and plasma antioxidant status in adult rats and their progeny. 相似文献
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