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Sadhana A. Samant Deepti D. Deobagkar N. N. Godbole Dileep N. Deobagkar 《Journal of genetics》1989,68(2):109-116
Sequences homologous to oncogeneyes (Y73/Esh/sarcoma viral oncogene cDNA) in theDrosophila melanogaster Oregon genome were detected byin situ hybridization on salivary gland chromosomes. Three separate sites, 8D/X, 57BC/2R and 95CD/3R, were identified. Presence of
sequences highly homologous toyes in the genomic DNA was confirmed by dot blot hybridization under high stringency conditions. 相似文献
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Dharmendra Singh Chandan Kumar Singh Ram Sewak Singh Tomar Jyoti Taunk Ranjeet Singh Sadhana Maurya Ashish Kumar Chaturvedi Pal Madan Singh Rajendra Dubey Sarawan Kumar 《PloS one》2016,11(1)
The success of drought tolerance breeding programs can be enhanced through molecular assortment of germplasm. This study was designed to characterize molecular diversity within and between Lens species with different adaptations to drought stress conditions using SSR markers. Drought stress was applied at seedling stage to study the effects on morpho-physiological traits under controlled condition, where tolerant cultivars and wilds showed 12.8–27.6% and 9.5–23.2% reduction in seed yield per plant respectively. When juxtaposed to field conditions, the tolerant cultivars (PDL-1 and PDL-2) and wild (ILWL-314 and ILWL-436) accessions showed 10.5–26.5% and 7.5%–15.6% reduction in seed yield per plant, respectively under rain-fed conditions. The reductions in seed yield in the two tolerant cultivars and wilds under severe drought condition were 48–49% and 30.5–45.3% respectively. A set of 258 alleles were identified among 278 genotypes using 35 SSR markers. Genetic diversity and polymorphism information contents varied between 0.321–0.854 and 0.299–0.836, with mean value of 0.682 and 0.643, respectively. All the genotypes were clustered into 11 groups based on SSR markers. Tolerant genotypes were grouped in cluster 6 while sensitive ones were mainly grouped into cluster 7. Wild accessions were separated from cultivars on the basis of both population structure and cluster analysis. Cluster analysis has further grouped the wild accessions on the basis of species and sub-species into 5 clusters. Physiological and morphological characters under drought stress were significantly (P = 0.05) different among microsatellite clusters. These findings suggest that drought adaptation is variable among wild and cultivated genotypes. Also, genotypes from contrasting clusters can be selected for hybridization which could help in evolution of better segregants for improving drought tolerance in lentil. 相似文献
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Alexey?TeplyakovEmail author Sadhana?Pullalarevu Galina?Obmolova Victoria?Doseeva Andrey?Galkin Osnat?Herzberg Miroslawa?Dauter Zbigniew?Dauter Gary?L?GillilandEmail author 《BMC structural biology》2004,4(1):5
Background
The yffB (PA3664) gene of Pseudomonas aeruginosa encodes an uncharacterized protein of 13 kDa molecular weight with a marginal sequence similarity to arsenate reductase from Escherichia coli. The crystal structure determination of YffB was undertaken as part of a structural genomics effort in order to assist with the functional assignment of the protein. 相似文献4.
A new immobilization matrix based on zeolite has been developed to immobilize Azotobacter chroococcum, for fixing nitrogen, with an intention to hold the cells in the root zone of the plants and to protect them under stressful
conditions. The matrix has been developed by modifying the surface of the zeolite with surfactant. This enhances the hydrophobicity
of the material and also modifies the surface charge, which in turn enhances the immobilization. Surface modified zeolite-A
(SMZ-A) has been compared with commercial zeolite-A (CZA) for immobilization efficiency. CZA is non-toxic for A. chroococcum but is inefficient to adsorb the cells whereas SMZ-A showed 100% adsorption of the microbial cells wherein it was observed
that for 1 l of broth culture with total viable count of 108 cfu ml−1 cells of A. chroococcum, a minimum dose of 0.7 g SMZ-A and minimum contact time of 10 h is required to achieve 100% adsorption. Adsorption was confirmed
by the cell count and light as well as scanning electron microscopy. Most importantly, the cells adsorbed on SMZ-A could fix
the atmospheric nitrogen up to 13 mg g−1 sucrose consumed, which was comparable with the control (unadsorbed cells), which confirms the survival and nitrogen fixation
activity of the bacteria.
Responsible Editor: Euan K. James. 相似文献
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Maternal nutrition is an important determinant of one-carbon metabolism that lies at the heart of intrauterine epigenetic programming. Exchange of nutrients and other vital molecules between the mother and fetus takes place across the placenta and hence may play direct role in fetal programming. Pre-eclampsia (PE) originates in the placenta and altered maternal nutrition may influence epigenetic patterns in the placenta, thereby affecting birth outcome. In the present study, we investigated the global DNA methylation levels in placentas of pre-eclampsia women (i.e., women delivering at term and those delivering preterm) and studied their associations with maternal blood pressure and birth outcome. Increased homocysteine and global DNA methylation levels were seen in the pre-eclampsia group (term and preterm PE) when compared with the normotensive group (p?0.05). A positive association between global DNA methylation and systolic (p?0.01) and diastolic (p?0.05) blood pressure was seen in the term pre-eclampsia group, whereas there was no association with birth outcome. The study for the first time provides evidence for altered global DNA methylation patterns in pre-eclampsia placentas and its association with blood pressure. It is possible that increased homocysteine levels may be related to increased methylation in pre-eclampsia. 相似文献
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Potential adverse effects of excess maternal folic acid supplementation on a vegetarian population deficient in vitamin B(12) are poorly understood. We have previously shown in a rat model that maternal folic acid supplementation at marginal protein levels reduces brain omega-3 fatty acid levels in the adult offspring. We have also reported that reduced docosahexaenoic acid (DHA) levels may result in diversion of methyl groups towards DNA in the one carbon metabolic pathway ultimately resulting in DNA methylation. This study was designed to examine the effect of normal and excess folic acid in the absence and presence of vitamin B(12) deficiency on global methylation patterns in the placenta. Further, the effect of maternal omega 3 fatty acid supplementation on the above vitamin B(12) deficient diets was also examined. Our results suggest maternal folic acid supplementation in the absence of vitamin B(12) lowers plasma and placental DHA levels (p<0.05) and reduces global DNA methylation levels (p<0.05). When this group was supplemented with omega 3 fatty acids there was an increase in placental DHA levels and subsequently DNA methylation levels revert back to the levels of the control group. Our results suggest for the first time that DHA plays an important role in one carbon metabolism thereby influencing global DNA methylation in the placenta. 相似文献
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