The effect of temperature on progeny allocation and short interval timing in a parasitoid wasp

Abstract The parasitoid wasp Trichogramma dendrolimi Matsumura sets the number of progeny allocated to its insect egg hosts according to the duration of its initial transit walk across the host surface. Although cooling the wasp reduces its walking speed, reduced temperature does not affect progeny allocation. By locally heating and cooling the wasps, the initial transit can be thermally uncoupled from the subsequent oviposition. Using this technique we show that the timing of initial transit duration is temperature-dependent. These findings suggest that short interval timing by Trichogramma differs physiologically from the temperature compensated clocks that have been described for other insects.     

Decomposition Analysis of Competitive Symmetry and Size Structure Dynamics

An analysis is introduced, based on the decomposition of relativegrowth rates, to examine the mode of competition (i.e. whethercompetition is symmetric or asymmetric), the size-dependenceof growth, and their interdependence. In particular, the basisfor two commonly held views is examined: (1) that the type ofresource limitation determines the mode of competition, and(2) that asymmetric competition always leads to size-divergencebetween unequal competitors. It is shown that in field-grownmillet plants, competition for light was symmetric at low densityand asymmetric at high density. However, size variation at lowdensity decreased during growth, because small plants had greaterrelative growth rates than larger plants. Size variation stayedconstant at high density, since plants of all sizes had equalaverage relative growth rates. Based on these results and ageneral discussion, it is proposed that the type of resourcelimitation does not determine the mode of competition. Competitionfor light can be symmetric, and foraging for heterogeneouslydistributed soil resources can produce asymmetric competitionbelow-ground. Furthermore, the mode of competition alone doesnot determine size structure dynamics. Size-dependence of resourceconversion efficiency and allocation can modify the effectsof resource uptake on growth. Pennisetum americanum‘Custer ’; mode of competition; size structure dynamics; plant growth analysis     

Improved Coated Vesicle Isolation Allows Better Characterization of Clathrin Polypeptides

Postmicrosomal pellets from plant sources are grossly contaminatedwith nbosomes. Previous purifications of clathrin coated vesicles(CCV) from such subcellular fractions have therefore often involvedan RNase treatment. Performed at 30°C, this step inherentlycarries with it the dangers of proteolysis. We document herea method for CCV isolation which avoids this. Through the inclusionof suitable antiproteases in the homogenizing and subsequentisolation media, we have also been able to improve the qualityof CCV recovered from plant tissues. As a result we have tentativelybeen able to identify clathrin light chains from zucchini hypocotyland pea cotyledon CCV. Similar to light chains from bovine brainthese polypeptides are heat stable, can be solubilized fromneutralized TCA precipitates, bind calcium and clathrin heavychains. However, in contrast to brain CCV the two light chainsof plant CCV are some 10 kDa heavier. Key words: Antiproteases, Ca²⁺-binding, clathrin coated vesicles, clathrin heavy chains, clathrin light chain(s), heat stability, pea cotyledons, RNase, zucchini hypocotyls