Aminopeptidases from Plasmodium falciparum, Plasmodium chabaudi chabaudi and Plasmodium berghei

ABSTRACT. Using fluorogenic substrates and polyacrylamide gels we detected in cell-free extracts of Plasmodium falciparum, Plasmodium chabaudi chabaudi and Plasmodium berghei only a single aminopeptidase. A comparative study of the aminopeptidase activity in each extract revealed that the enzymes have similar specificities and kinetics, a near-neutral pH optima of 7.2 and are moderately thermophilic. Each has an apparent molecular weight of 80,000 ± 10,000, determined by high performance liquid chromatography on a calibrated SW500 column. Whilst the P. c. chabaudi and P. berghei activity co-migrate in native polyacrylamide gels, that of P. falciparum migrates more slowly. The three enzymes can be selectively inhibited by ortho -phenanthroline and are thus metallo-aminopeptidases; however, in contrast to other aminopeptidases the metal co-factor does not appear to be Zn²⁺.     

Ocean-scale patterns of 'biodiversity' of Atlantic asteroids determined from taxonomic distinctness and other measures

We examine patterns of ‘gamma’ (within-rcgion) and ‘beta’ (between-region) diversity from analysis of a presence/absence dataset for species of asteroids encompassing the whole Atlantic Ocean partitioned into 26 regions. Absolute species numbers (a poor measure of biodiversity) in shallow coastal areas and the deep sea are the same, although species richness per area for two well-sampled regions suggests, qualitatively, that coastal areas may be more speciose. Taxonomic distinctness (A*), an index which is markedly less sample-size dependent than other common diversity measures, shows no significant association with geographic area and no clear pattern with depth, suggesting an absence of latitudinal and coastal/deep trends. Cluster analysis shows that distinctive faunal assemblages are most evident in shelf/ shallow waters, where six groupings separate recognizably acccording to geographical location. Three of these arc the southernmost regions of the Atlantic (southeast of S. America, S. Angola/S. Africa & Tristan da Cunha/Gough Island) and arc characterized by their isolation and high levels of endemism. As depth increases so does the amount of faunal similarity between regions. This indicates that beta diversity is highest in shelf regions and lowest in lower bathyal/abyssal regions. Our results may support the contention which questions the emerging paradigm that the deep sea has exceptionally high diversity. It is evident, however, that comparisons (e.g. between coasts and the deep sea) are problematic and can depend very much on the element(s) of biodiversity measured, sampling methods and the spatial scales (e.g. alpha, beta or gamma diversity) over which assessment is made. Any wider conclusions should therefore be drawn cautiously, particularly since assessment is made of only one faunal group. Other findings include significant correlation between the depth range of asteroids and their geographical range. The utility of low-resolution datasets is reviewed. It is concluded that within limitations they can be of value for determining broad (e.g. regional, ocean-scale and global-scale) patterns of diversity and community structure, especially when analysed using measures relatively uninfluenced by sample size.     

Survival of Salmonella dublin in Pig Slurry During Aerobic Thermophilic Treatment in Batch, Cyclic and Continuous Systems

The survival of Salmonella dublin was investigated in aerobically fermenting and autoclaved pig slurry at a temperature of 55°C. The difference in the decay rates for these two situations was not statistically significant. Initial bacterial concentrations of about 10⁶/ml were reduced to undetectable levels in less than 4 h in both cases, the decimal decay rate based on both sets of data was 1.81/h. Theoretical analysis, based on the decay rate derived from these results, indicated that cyclic feeding and discharging had advantages over the continuous system particularly as retention time increased.     

Thermophilic Fermentation of Pig Faeces and Straw by Actinomycetes

The ability of Thermomonospora fusca, Thermomonospora curvata and Pseudonorcardia thermophila to grow on and hydrolyse pig faeces and straw was studied in a 6 d batch culture at 55°C. T. fusca produced the highest levels of cellulase activity (3·3 mg/ml/h) and the greatest cellulose reduction (from 25 to 6% dry wt) in a pig faeces medium (10 g/l). Replacing half the pig faeces with grass straw reduced the cellulose breakdown (29 to 18% dry wt). Increasing the concentration of pig faeces to 30 and 50 g/l caused a decrease in cellulose breakdown. To achieve similar cellulose reductions in straws required NaOH pretreatment. All fermentations resulted in significant increases in digestible protein. The celluloses produced by the strains growing on pig faeces exhibited greatest activity in the pH range 5·9–6·4.