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Plant Regeneration Through Meristem Culture from Vegetative Buds of Mulberry (Morus bombycis Koidz.) Stored in Liquid Nitrogen 总被引:3,自引:0,他引:3
Intact vegetative buds of mulberry (Morus bombycis) attachedto shoot segments were prefrozen, stored in liquid nitrogen,thawed, and the meristems excised for culture on Murashige andSkoog's medium supplemented with 1 mg 11 BA to regenerateplants. Either prefreezing at 10 °C or 20°C along with rapid thawing at 37 °C or prefreezingat 20 °C or 30 °C along with slow thawingat 0 °C was a suitable condition for high percentages ofsurvival and shoot regeneration. Potted mulberry plants couldbe finally obtained from the cryopreserved material. The systemusing intact buds as the material for cryopreservation is quitesimple when compared to conventional systems using isolatedmeristems together with cryoprotectants. Morus bombycis Koidz., mulberry, cryopreservation, meristem culture, plant regeneration 相似文献
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