Melanin Granules Prevent the Cytotoxic Effects of l‐DOPA on Retinal Pigment Epithelial Cells in Vitro by Regulation of NO and Superoxide Radicals

Inasmuch as the nitrogen cycle elicits the direct reduction of N₂ to NH₃ through enzymatic reactions, and inasmuch as l ‐DOPA ( l ‐dihydroxyphentlalamine), a catecholamine, can be a source of nitric oxide (NO), it is possible that melanin granules in the eye affect the generation of NO, which causes damage to the retinal pigment epithelial (RPE) cells during the oxidation of l ‐DOPA. In order to confirm this possibility, we analyzed the correlations of NO generation, cell growth, and superoxide dismutase (SOD) activities in two types (melanotic and amelanotic) of bovine RPE cells following exposure to l ‐DOPA. NO generation from l ‐DOPA was determined using an NO detector that is reliant on redox currents. The concentration of NO was measured in terms of diffusion currents run between a working electrode and a counter electrode, both being set in culture medium placed in a Petri dish. For the assays, l ‐DOPA was added to the medium at various concentrations (5, 29.9, 79.4, 152.7 or 249 μM), and 6 min after addition, an NO‐trapping agent 2,4‐carboxyphenyl‐4,4,5,5‐tetramethylimidazole‐1‐oxyl 3‐oxide (carboxy‐PTIO) was also added. The melanotic and amelanotic types of RPE cells were cultured separately in medium with l ‐DOPA under an atmosphere containing 20, 10 or 5% oxygen. Cell numbers were counted using a Coulter counter, and SOD activities were determined following incubation for 24, 48 or 72 hr using a modification of the luminol assay. The results obtained indicated that: (a) NO was produced from l ‐DOPA in a concentration‐dependent manner and was trapped quantitatively by carboxy‐PTIO; (b) the generation of NO was inhibited more markedly in the melanotic cell line than in the amelanotic one, suggesting an increased tolerance to l ‐DOPA‐derived cytotoxicity in the former; and (c) the SOD activities were more affected by oxygen concentration in the melanotic cells than in the amelanotic ones. From these results, it is concluded that melanin granules in RPE cells have a role in preventing the cytotoxicity derived from l ‐DOPA and in regulating the generation of NO and superoxide radicals.