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Cultures under nitrogen limitation for Chlorella vulgaris were kept under different light quality (white, blue, yellow and violet) at 70 and 140 µE m?2 s?1; to evaluate the effect on fatty acids profiles and biodiesel quality. The results showed a maximum biomass and cell density at 140 µE m?2 s?1 of: white light (0.69 g L?1 and 6.5?×?106 cells mL?1, respectively) and blue light (0.65 g L?1 and 8.0?×?106 cells mL?1, respectively); compared to violet and yellow light. The chlorophyll concentration (µg mg?1 biomass dry weight) at 70 µE m?2 s?1 were in the order of light: white (25.61)?>?violet (17.10)?>?yellow (11.68)?>?blue (11.40) and, at 140 µE m?2 s?1 were: violet (23.64)?>?white (10.20)?>?yellow (9.66)?>?blue (7.99), suggesting the violet light stimulates the increase of chlorophyll a at higher intensity. The maximum lipid content (% w/w) were present under blue light (43.11), yellow (70.92) and violet (83.87) at 140 µE m?2 s?1. The different wavelengths did not have a negative effect on the quality of the biodiesel, however; violet light presented greater productivity and the indicators such as CFPP were related to the oxidative stability value and low PUFA content, leading biodiesel to good oxidative stability.

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