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E. R. R. Rochedo L. F. C. Conti H. G. Paretzke 《Radiation and environmental biophysics》1996,35(4):243-261
The structure and mathematical model of PARATI, a detailed computer programme developed for the assessment of the radiological
consequences of an accidental contamination of urban areas, is described with respect to the scenarios used for the estimation
of exposure fields in a village or town, the models for the initial and secondary contamination with the radionuclide 137Cs, the concepts for calculating the resulting radiation exposures and the changes with time of the contamination and radiation
fields. Kerma rates at various locations in tropical urban areas are given, and the contribution of different contaminated
surfaces to these rates after dry or wet deposition are discussed.
Received: 12 April 1996 / Accepted in revised form: 30 August 1996 相似文献
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The polar lipids of the autotrophically grown, obligately anaerobic, photosynthetic bacterium Chromatium strain D were separated by paper chromatography. Four major phospholipids were identified: lysophosphatidylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, and cardiolipin. In addition, three glycolipids were observed and characterized, namely, monoglucosyldiglyceride, which is found in other biological systems, and (mannosyl, glucosyl)-diglyceride and (dimannosyl, glucosyl)-diglyceride, which heretofore have not been observed in nature. 相似文献
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T Aureli C Puccetti M E Di Cocco A Arduini R Ricciolini M Scalibastri C Manetti F Conti 《European journal of biochemistry》1999,263(1):287-293
The biochemical pathways involved in acetyl-L-carnitine utilization were investigated in conscious, freely moving rats by 13C NMR spectroscopy. Following 4-h [(1,2-13C2)acetyl]-L-carnitine infusion in fasted animals, the free carnitine levels in serum were increased, and an efflux of unlabelled acetyl-L-carnitine from tissues was observed. [(1,2-13C2)Acetyl]-L-carnitine was found to enter biosynthetic pathways in liver, and the acetyl moiety was incorporated into both cholesterol and 3-hydroxybutyrate carbon skeleton. In accord with the entry of [(1,2-13C2)acetyl]-L-carnitine in the mitochondrial acetylCoA pool associated with tricarboxylic acid cycle, the 13C label was also found in liver glutamate, glutamine, and glutathione. The analysis of the 13C-labelling pattern in 3-hydroxybutyrate and cholesterol carbon skeleton provided evidence that the acetyl-L-carnitine-derived acetylCoA pool used for ketone bodies synthesis in mitochondria was homogeneous, whereas cholesterol was synthesized from two different acetylCoA pools located in the extra- and intramitochondrial compartment, respectively. Furthermore, cholesterol molecules were shown to be preferentially synthesized by the metabolic route involving the direct channelling of CoA-activated mitochondria-derived ketone bodies into 3-hydroxy-3-methylglutarylCoA pathway, prior to equilibration of their acyl groups with extramitochondrial acetylCoA pool via acetoacetylCoA thiolase. 相似文献
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A new wild type of beta-lactoglobulin has been identified in the milk of sheep. It has been designated as ovine beta-lactoglobulin C. Its primary structure has been determined by direct protein microsequencing of intact protein and RP-HPLC-derived tryptic peptides. The new beta-lactoglobulin C is a subtype of ovine beta-lactoglobulin A with a single exchange Arg-Gln at position 148. This exchange may influence polymerisation of beta-lactoglobulin since in the crystal structure of orthorhombic bovine beta-lactoglobulin, residues 145-150 constitute a short beta-sheet region involved in dimer formation by pairing of dyad-related strands. 相似文献
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An ionotropic phase transition in phosphatidylcholine: cation and anion cooperativity 总被引:2,自引:0,他引:2
Evidence is presented for cooperative interaction between cations and anions specifically bound to dimyristoylphosphatidylcholine (DMPC). The cooperativity is with regard to an ion-induced (ionotropic) phase transition for the lipid and is signalled by a change in the luminescence from bound Tb3+. The intrinsic binding of Tb3+ to DMPC was determined from equilibrium dialysis experiments, using conventional methods to correct for electrostatic contributions. Preliminary results demonstrate great potential for infrared spectroscopy as a means to relate these Tb3+ luminescence studies to experiments involving less tractable cations. This work provides insight into the role of bound ions in modifying lateral phase behavior in phospholipid membranes. 相似文献
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Biotyping of pathogenic yeasts and hyphomycetes based on their suceptibility to selected killer yeasts and their reactivity with monoclonal antibodies are described. Both methods were used to differentiate fungi isolated from patients providing valuable epidemiological information on mycotic infections. The functional biotyping obtained with the two systems and the conventional auxenographic biocoding approaches commercially available for opportunistic yeasts are comparatively evaluated. The potential for biotyping of industrial fungal isolates is also discussed. 相似文献