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Fang Zhou Wei Zhao Zhixiang Zuo Yue Sheng Xiang Zhou Yu Hou Hanhua Cheng Rongjia Zhou 《The Journal of biological chemistry》2010,285(47):37030-37040
Androgen receptor (AR) plays a critical role in prostate cancer and male sexual differentiation. We have identified AR from a primitive vertebrate with a sex reversal characteristic, the rice field eel. AR of this species (eAR) is distinct from human AR, especially in the ligand binding domain (LBD), and its expression in gonads shows an increasing tendency during gonadal transformation from ovary via ovotestis to testis. eAR has a restricted androgen-dependent transactivation function after a nuclear translocation upon dihydrotestosterone exposure. A functional nuclear localization signal was further identified in the DNA binding domain and hinge region. Although nuclear export is CRM1-independent, eAR has a novel nuclear export signal, which is negatively charged, indicating that a nuclear export pathway may be mediated by electrostatic interaction. Further, our studies have identified critical sequences for ligand binding in the C terminus. A structure of three α-helices in the LBD has been conserved from eels to humans during vertebrate evolution, despite a distinct amino acid sequence. Mutation analysis confirmed that the LBD is essential for dihydrotestosterone-induced nuclear import of eAR and following transactivation function in the nucleus. In addition, eAR interacts with both Sox9a1 and Sox9a2, and their interaction regulates transactivation of eAR. Our data suggest that the primitive species conserves and especially acquires key novel domains, the nuclear export signal and LBD, for the eAR function in spite of a rapid sequence evolution. 相似文献
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Chinese sturgeon (Acipenser sinensis) is a rare and endangered species and also an important resource for the sturgeon aquaculture industry, however, a few genes
have been identified in this species. We report here construction of a pituitary cDNA library from a 24 years old female Chinese
sturgeon just after its spawning, and obtained 2,025 ESTs from the library. 885 unique sequences were identified, which were
categorized into 12 functional groups. More than half of the unique sequences (57%) do not match with annotated sequences
in the public databases. Three of these novel genes were further identified. Notably, a full-length of cDNA (1,143 bp) encoding
somatolactin of 232 amino acids was identified. Phylogenetic analysis showed 97% amino acid identity with White sturgeon somatolactin.
RT-PCR analysis indicated that the somatolactin mRNA was only detected in pituitary. Pituitary-specific expression of the
somatolactin suggested that the protein may play important physiological functions in pituitary-endocrine system of the Chinese
sturgeon. 相似文献
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Rongjia Mao Jing Chen Yuejun Chen Zhigang Guo 《Physiology and Molecular Biology of Plants》2018,24(5):715-727
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