全文获取类型
收费全文 | 2614篇 |
免费 | 234篇 |
国内免费 | 1篇 |
专业分类
2849篇 |
出版年
2023年 | 9篇 |
2022年 | 21篇 |
2021年 | 35篇 |
2020年 | 12篇 |
2019年 | 26篇 |
2018年 | 21篇 |
2017年 | 33篇 |
2016年 | 53篇 |
2015年 | 113篇 |
2014年 | 109篇 |
2013年 | 182篇 |
2012年 | 188篇 |
2011年 | 187篇 |
2010年 | 119篇 |
2009年 | 100篇 |
2008年 | 153篇 |
2007年 | 141篇 |
2006年 | 166篇 |
2005年 | 165篇 |
2004年 | 167篇 |
2003年 | 133篇 |
2002年 | 140篇 |
2001年 | 50篇 |
2000年 | 30篇 |
1999年 | 57篇 |
1998年 | 47篇 |
1997年 | 22篇 |
1996年 | 24篇 |
1995年 | 24篇 |
1994年 | 21篇 |
1993年 | 26篇 |
1992年 | 23篇 |
1991年 | 14篇 |
1990年 | 18篇 |
1989年 | 25篇 |
1988年 | 17篇 |
1987年 | 15篇 |
1986年 | 15篇 |
1985年 | 10篇 |
1984年 | 25篇 |
1983年 | 16篇 |
1982年 | 12篇 |
1981年 | 15篇 |
1980年 | 9篇 |
1979年 | 8篇 |
1978年 | 9篇 |
1977年 | 6篇 |
1976年 | 5篇 |
1974年 | 8篇 |
1971年 | 5篇 |
排序方式: 共有2849条查询结果,搜索用时 15 毫秒
1.
Mirror carp were infected with Ichthyophthirius multifiliis (Fouquet) under standardized conditions. The size and number of parasites at selected sites on the body were recorded during the course of the infection. Initial exposure to 40 mature parasites resulted in a mild infection with 100% recovery after 18 days. Recovered fish did not appear to be carriers of the parasite. Exposure to 400 parasites resulted in 100% mortality between 22–25 days. The growth rate of the parasite was linear. Parasites were more numerous in the dorsal surface of the fish than in the lateral or ventral surface. The increase in parasite numbers during the disease was greater in the gills than in the skin. 相似文献
2.
Cascade J. B. Sorte Ron J. Etter Robert Spackman Elizabeth E. Boyle Robyn E. Hannigan 《PloS one》2013,8(11)
As the climate warms, species that cannot tolerate changing conditions will only persist if they undergo range shifts. Redistribution ability may be particularly variable for benthic marine species that disperse as pelagic larvae in ocean currents. The blue mussel, Mytilus edulis, has recently experienced a warming-related range contraction in the southeastern USA and may face limitations to northward range shifts within the Gulf of Maine where dominant coastal currents flow southward. Thus, blue mussels might be especially vulnerable to warming, and understanding dispersal patterns is crucial given the species'' relatively long planktonic larval period (>1 month). To determine whether trace elemental “fingerprints” incorporated in mussel shells could be used to identify population sources (i.e. collection locations), we assessed the geographic variation in shell chemistry of blue mussels collected from seven populations between Cape Cod, Massachusetts and northern Maine. Across this ∼500 km of coastline, we were able to successfully predict population sources for over two-thirds of juvenile individuals, with almost 80% of juveniles classified within one site of their collection location and 97% correctly classified to region. These results indicate that significant differences in elemental signatures of mussel shells exist between open-coast sites separated by ∼50 km throughout the Gulf of Maine. Our findings suggest that elemental “fingerprinting” is a promising approach for predicting redistribution potential of the blue mussel, an ecologically and economically important species in the region. 相似文献
3.
Monoterpenoids from wood of Pinus quadrifolia and Pinus monophylla from south of the San Jacinto Mountains in southern California were analysed by gas-liquid chromatography (GLC). The number of needles per fascicle, the number of resin canals, and the number of abaxial and adaxial stomatal rows in the needles were determined. Percentages of mycrene, α-pinene, and to a minor extent of camphene, β-pinene, limonene, and β-phellandrene, the above mentioned morphological characteristics and previously obtained data were all used for identification and characterisation of species intermediacy in the San Jacinto area. It was concluded that many P. quadrifolia trees in the mixed Buck Ridge stand and some trees in two pure stands of the same area were hybrids and that sympatric and to a lesser extent allopatric introgression of P. monophylla into P. quadrifolia takes place in the San Jacinto region. Introgression of P. quadrifolia into P. monophylla could not be demonstrated. 相似文献
4.
5.
Abstract Azide, an inhibitor of ATPase, and a specific inhibitor of protein export was used in order to select for protein secretion mutants in Acinetobacter calcoaceticus A2. Two such mutants were isolated that were azide-resistant and defective in the general protein transport system. The mutation also conferred additional phenotypic changes, including an inability to grow on minimal media or at 40°C. The existence of protein secretion mutants with a selectable phenotype may be useful for the genetic study of protein export. 相似文献
6.
The effect of a segregating economic trait locus (ETL) can be detected with the aid of a linked genetic marker, if specific alleles of each locus are in association among the individuals genotyped for the genetic marker. For dairy cattle this can be achieved by application of the ‘granddaughter design’. If only the sires and their sons are genotyped for the genetic markers, then the allele origin of sons having the same genotypes as their sires cannot be determined. Seven sires and 101 sons were genotyped for five microsatellites. The mean frequency of heterozygous sires was 77%. The mean number of alleles per locus was 8.2. Frequency of informative sons per locus ranged from 60% to 80% with a mean of 72%. With highly polymorphic microsatellites, at least 60% more grandsire families can be included in the analysis, and the number of sons assayed can be reduced by 40%, as compared to diallelic markers. 相似文献
7.
Herman van der Kooij Ron Jacobs Bart Koopman Henk Grootenboer 《Biological cybernetics》1999,80(5):299-308
A model is presented to study and quantify the contribution of all available sensory information to human standing based
on optimal estimation theory. In the model, delayed sensory information is integrated in such a way that a best estimate of
body orientation is obtained. The model approach agrees with the present theory of the goal of human balance control. The
model is not based on purely inverted pendulum body dynamics, but rather on a three-link segment model of a standing human
on a movable support base. In addition, the model is non-linear and explicitly addresses the problem of multisensory integration
and neural time delays. A predictive element is included in the controller to compensate for time delays, necessary to maintain
erect body orientation. Model results of sensory perturbations on total body sway closely resemble experimental results. Despite
internal and external perturbations, the controller is able to stabilise the model of an inherently unstable standing human
with neural time delays of 100 ms. It is concluded, that the model is capable of studying and quantifying multisensory integration
in human stance control. We aim to apply the model in (1) the design and development of prostheses and orthoses and (2) the
diagnosis of neurological balance disorders.
Received: 25 August 1997 / Accepted in revised form: 8 December 1998 相似文献
8.
Ron J. Etter 《Evolution; international journal of organic evolution》1988,42(2):322-334
Field and laboratory experiments were used to investigate the variation and phenotypic plasticity in the adhesive abilities of the intertidal snail Nucella lapillus between high- and low-wave-energy environments. Whelks from an exposed coast produced a larger pedal surface area and were more resistant to dislodgement than were similar-sized individuals from a protected shore. Tenacity (g/cm2) was similar between individuals from exposed and protected shores, indicating that variation in resisting dislodgement was solely a function of pedal surface area. Whelks from exposed and protected shores did not differ in pedal surface area as they emerged from egg capsules or when reared in the laboratory under uniform conditions, suggesting that variation between populations does not represent genetic differentiation. Individuals from high-and low-wave-energy environments reared intertidally produced a larger pedal surface area than did those reared in the laboratory. The extent to which pedal surface area increased corresponded to the intensity of wave action. These findings suggest that pedal surface area is a highly plastic character modulated or induced by the water turbulence accompanying breaking waves. A reciprocal-transplant experiment confirmed this notion but revealed an asymmetry in the plasticity. Snails from the protected site transplanted to an exposed shore formed a much larger pedal surface area than did controls reared on the protected shore. In contrast, whelks from a wave-swept shore transplanted to a protected shore differed little from their controls reared on the exposed shore. The asymmetric response parallels a possible asymmetry in the risks of acclimating to a temporally unpredictable environmental cue, such as wave action. 相似文献
9.
Transgenic HLA-DR alpha faithfully reconstitutes IE-controlled immune functions and induces cross-tolerance to E alpha in E alpha 0 mutant mice 总被引:5,自引:0,他引:5
We have constructed transgenic mice that express the human class II MHC molecule HLA-DR alpha on a genetic background in which the equivalent endogenous gene, H-2 IE alpha, is not expressed. In these mice, DR alpha complemented the E beta chain such that tissue-specific expression of an interspecies hybrid DR alpha-E beta heterodimer was obtained. Despite 25% amino acid differences between DR alpha and E alpha, immune responsiveness to IE-controlled antigens, clonal deletion of IE-reactive T cells, and alloantigenicity were quantitatively and qualitatively indistinguishable in IE-positive mice and in mice that had integrated at least four copies of the transgene. These results demonstrate a remarkable degree of structural, regulatory, and functional conservation. They also suggest that tolerance induction involves only discrete portions of MHC molecules. 相似文献
10.
Summary Two pairs of ganglia are found in the propodial region of the veliger of Onchidoris bilamellata: the anterolateral pair is located at the foremost corners of the propodium, and the frontal pair is located beside the propodial midline. Both sets of ganglia are positioned below the epidermis, and they are joined to the cerebral ganglia by large, common connectives. Each ganglion possesses sensory cells, nerve cells and sheath cells, and the frontal pair contains a complement of secretory cells. Externally, the propodial ganglia are manifested as sensory fields. The fields of the anterolateral pair are elliptical in shape, and each appears as a band of cilia bordering an unciliated zone. The region devoid of cilia is composed of ordinary epidermal cells, whereas the ciliated portion is comprised of dendritic endings originating from cells in the ganglion. Dendrites arise from one type of sensory cell and pass through the epidermis in bundles. Each dendrite terminates as a single cilium at the epidermal surface. Sensory fields of the frontal ganglia are key-shaped and oppose one another on the anterior end of the foot. Each field appears as a flat, circular, unciliated region which extends into a ciliated groove that runs dorsally toward the mouth. The groove contains the terminals of secretory cells, ciliated sensory cells, and the cell bodies of nonciliated sensory cells. The nonciliated sensory cells, characterized by a microvillous apex, are the dominant cells in the flattened circular zone. The space between the frontal ganglia and the epidermis is bridged by bundles of processes which are similar to those of the anterolateral ganglia. However, these tracts contain collections of the apical processes of secretory cells, the dendrites of ciliated sensory cells, and the axons of nonciliated sensory cells. Morphological and behavioral evidence indicates that the propodial ganglia serve a chemosensory function during settlement and metamorphosis. 相似文献