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1.
Drosophila littoralis overwinters in the adult stage in a reproductive diapause. During the warm season there are one or two generations in Finland. The diapause appears to be a prolongation of the post-eclosion immaturity of young females. The termination of diapause is controlled by a combination of adequate temperature and sufficiently long photophases. The diapausing status of females is ascertained by inspecting the developmental stage of their ovaries. In laboratory experiments the maturity of ovaries is not closely correlated with the receptivity of females.  相似文献   
2.
The induction of liver microsomal monooxygenase activities elicited by pyrazole, ethanol, and acetone, all shown to be inducers of rat P450j and rabbit P450LM3a, has been compared in inbred strains of DBA/2N, AKR/J, and Balb/c mouse. Pyrazole strongly increases coumarin 7-hydroxylase (COH) activity in DBA/2N but much less in other strains. The effect of pyrazole on aniline p-hydroxylase and ethanol oxidase activities is also strain dependent: an increase was seen only in the DBA/2N strain. Ethanol and acetone were unable to induce COH, whereas aniline p-hydroxylase and ethanol oxidase were elevated about 1.4- to 3.3-fold in all strains. No strain difference could be detected in aniline p-hydroxylase or ethanol oxidase inducibility. There was a strong correlation between aniline p-hydroxylase and ethanol oxidase activities in every strain, whereas no positive correlation could be found between COH and aniline p-hydroxylase activities. Immunoinhibition experiments showed that a polyclonal antibody against purified pyrazole-inducible COH (P450Coh) blocked about 90% of COH activity, but only about 10% of aniline p-hydroxylase or ethanol oxidase in mouse liver microsomes. Monoclonal antibody 1-91-3 (raised against rat acetone-inducible P450ac) did not inhibit COH, whereas aniline p-hydroxylase was blocked 46-76% and ethanol oxidase 25-70%, depending on the source of microsomes. In immunoblots, anti-P450Coh recognized only its own antigen but not the P450ac, whereas monoclonal antibody 1-98-1 against P450ac detected P450ac and a corresponding form in the D2 mouse liver, but not the P450Coh. The purified P450ac and P450Coh had molecular masses of 52 and 50 kDa, respectively, on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These antigens were expressed differentially in response to pyrazole, ethanol, and acetone: P450Coh was increased only after pyrazole treatment, but 1-98-1-detectable protein was elevated in D2 mouse liver microsomes by ethanol and acetone, but not by pyrazole. We conclude that mouse P450Coh and rat P450ac are not corresponding forms of the same isozyme, and that a P450ac-like protein, responsible for most of aniline p-hydroxylation and ethanol oxidation, is present in the D2 mouse liver. These two P450 isozymes are also dissimilarly expressed in the mouse liver in response to inducer administration.  相似文献   
3.
Selective induction of coumarin 7-hydroxylase by pyrazole in D2 mice   总被引:3,自引:0,他引:3  
Pyrazole, was given to DBA/2N (D2), C57BL/6N (B6) and AKR/N mice to study its effects on hepatic drug metabolism. A decrease in the total amount of microsomal cytochrome P-450 as well as in the activities of ethylmorphine demethylase and benzo[a]pyrene hydroxylase was found. On the other hand ethoxycoumarin de-ethylase was increased 1.5-2.5-fold (depending on the strain of mouse) and coumarin 7-hydroxylase as much as sevenfold (but only in D2 mice) after pyrazole treatment. This increase was much higher than that caused by phenobarbital, the only well known inducer of coumarin 7-hydroxylase. By reconstituting the mono-oxygenase complex after purification of cytochrome P-450 we found a 40-fold increase in coumarin 7-hydroxylase and eightfold increase in ethoxycoumarin de-ethylase after pyrazole treatment. This was found only in D2 mice. An antibody previously developed against a cytochrome P-450 fraction from the the D2 strain with a high coumarin 7-hydroxylase activity inhibited the microsomal coumarin 7-hydroxylase almost 100% after pyrazole pretreatment of the animals. In the case of control or phenobarbital-treated mice the inhibition was somewhat weaker. With the reconstituted mono-oxygenase complex the inhibition of coumarin 7-hydroxylase was almost 100% both for control and pyrazole-treated D2 mice. The data indicate that pyrazole causes an induction of the microsomal monooxygenase complex different from that caused by phenobarbital or 3-methylcholanthrene and selective for coumarin 7-hydroxylation or 7-ethoxycoumarin de-ethylation. This induction was strong in D2, weak in B6 and absent in AKR/N mice.  相似文献   
4.
Lymphoid cell subclasses in rejecting renal allograft in the rat   总被引:4,自引:0,他引:4  
We have quantitated the frequency of lymphoid cell subsets in rejecting renal allografts and in the spleen of the allograft recipient during drug-unmodified rejection in the rat. The number of inflammatory (white) cells in the graft was approximately similar to the number of white cells responding to the allograft in the recipient spleen. The inflammatory population of the graft consisted of lymphoid cells and mononuclear phagocytes, with increasing numbers of macrophages toward the end of rejection. Analysis of allograft cellular dispersates with monoclonal antibodies directed to the lymphoid cell subsets demonstrated that although the majority of allograft-infiltrating lymphocytes were T cells, a sizable B-cell proliferation and immunoglobulin synthesis was associated with the inflammatory response of rejection. Within the T-cell subset, the T suppressor/killer cells predominated in the graft whereas the predominant lymphoid cell subset responding to the allograft in the recipient spleen was the T helper cell.  相似文献   
5.
This study presents a quantitative partitioning of the total variance in the patterns of occurrence of 231 vascular plant taxa in 362 1 × 1 km grids in the Kevo Nature Reserve into four independent components: purely spatial variation, spatially structured environmental variation, non-spatial environmental variation, and unexplained variation. This partitioning is done with (partial) constrained ordinations (canonical correspondence analysis) and associated Monte Carlo permutation tests. The numerical results suggest that most of the biological variance captured by the external explanatory variables is related to 'local' meso-scale environmental factors, as 12.6% of the variation in the species data is explained solely by the environmental variables. Part of the variance (6%) represents a spatially covarying environmental component, but only a very small part, ca 2%, is related to purely spatial variation. The amount of unexplained variation is very high (>75%). The results are compared and discussed in relation to the relative amounts of these four variance components at broader- and finer-scales and to the concepts of domains and transition zones of scales in biological patterning.  相似文献   
6.
Identifying resuspended particles using isotope ratios   总被引:5,自引:2,他引:3  
Three simple methods were developed to estimate the proportion of particles in lake water derived from resuspended material. These techniques use the different distributions of long and short-lived radioisotopes sorbed onto particles and were tested in the three basins of Lake Erie using 7Be, 137Cs and 210Po/210Pb.While the concentration of 210Po on particles did not vary significantly in the lake, resuspended particles were characterized by high concentrations of 137Cs and low concentrations of 7Be. The distribution of these radioisotopes is consistent with a simple mixing model in which the fraction of particles in the lake water derived from resuspension ranged from 8% to about 100%. Higher concentrations of resuspended particles were found in deeper samples from the nepheloid layer and in the shallow western basin where thermal stratification was very weak.  相似文献   
7.
The relationship of the macrozoobenthos biomass in the littoral area to the yearly fluctuation in water level and the characteristics of the area or lake are studied using data collected from sheltered bays in regulated and natural waters. Most of the lakes were clear and oligotrophic. The benthos biomass at all depths in the littoral decreased with increased water level fluctuation, provided that the transparency of the water was uniform.The macrozoobenthos biomass in the 0–3 m depth zone could be predicted fromlog macrozoobenthos biomass (mg ODW) m-2=4.25-1.33 (log Biomass Index) in which the Biomass Index is calculated as% MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr% 4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq-Jc9% vqaqpepm0xbba9pwe9Q8fs0-yqaqpepae9pg0FirpepeKkFr0xfr-x% fr-xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaGaaeOqaiaabM% gacaqGVbGaaeyBaiaabggacaqGZbGaae4CaiaabccacaqGjbGaaeOB% aiaabsgacaqGLbGaaeiEaiaab2dacaqGGaGaaeiiaiaabccacaqGGa% GaaeiiaiaabccacaqGGaGaaeiiaiaabccacaqGGaGaaeiiaiaabcca% caqGGaGaaeiiaiaabccacaqGGaGaaeiiaiaabccacaqGGaGaaeiiai% aabccadaWcaaabaeqabaGaae4DaiaabggacaqG0bGaaeyzaiaabkha% caqGGaGaaeiBaiaabwgacaqG2bGaaeyzaiaabYgacaqGGaGaaeOzai% aabYgacaqG1bGaae4yaiaabshacaqG1bGaaeyyaiaabshacaqGPbGa% ae4Baiaab6gacaqGGaGaaeyAaiaab6gacaqGGaGaaeiDaiaabIgaca% qGLbGaaeiiaiaabchacaqGYbGaaeyzaiaabAhacaqGPbGaae4Baiaa% bwhacaqGZbGaaeiiaiaabMhacaqGLbGaaeyyaiaabkhaaeaacaqGOa% GaaeyBaiaabUdacaqGGaGaae4yaiaabggacaqGSbGaae4yaiaabwha% caqGSbGaaeyyaiaabshacaqGLbGaaeizaiaabccacaqGMbGaaeOCai% aab+gacaqGTbGaaeiiaiaab2gacaqGVbGaaeOBaiaabshacaqGObGa% aeiBaiaabMhacaqGGaGaaeyBaiaabwgacaqGHbGaaeOBaiaabccaca% qG2bGaaeyyaiaabYgacaqG1bGaaeyzaiaabohacaqGPaaaaeaacaqG% tbGaaeyzaiaabogacaqGJbGaaeiAaiaabMgacaqGGaGaaeizaiaabM% gacaqGZbGaae4AaiaabccacaqG2bGaaeyyaiaabYgacaqG1bGaaeyz% aiaabccacaqGPbGaaeOBaiaabccacaqG0bGaaeiAaiaabwgacaqGGa% Gaae4CaiaabggacaqGTbGaaeyzaiaabccacaqGVbGaaeiCaiaabwga% caqGUbGaaeiiaiaabEhacaqGHbGaaeiDaiaabwgacaqGYbGaaeiiai% aabohacaqGLbGaaeyyaiaabohacaqGVbGaaeOBaiaabccacaqGOaGa% aeyBaiaabMcaaaaccaGae8hiaaIaaKiEaiab-bcaGiaaigdacaaIWa% GaaGimaiaac6caaaa!CBD8!\[{\text{Biomass Index = }}\frac{\begin{gathered} {\text{water level fluctuation in the previous year}} \hfill \\ {\text{(m; calculated from monthly mean values)}} \hfill \\ \end{gathered} }{{{\text{Secchi disk value in the same open water season (m)}}}} \user1{x} 100.\]The whole illuminated littoral shifts due to water level fluctuation, which disturbs the zonation of the benthos. Such an increase or decrease in benthic biomass has been observed after one year of disturbance due to water level fluctuation. It need, however, a study based on the carefully planned and collected data, in which it can be taken account by a multivariate statistical analysis also the interactions between the important factors affected the littoral benthos.  相似文献   
8.
Abstract: Cerebral glutamate was monitored in a superfused cerebral cortical preparation by 1H NMR spectroscopy using a semiselective spin-echo sequence N -acetyl aspartate (NAA) as an internal concentration reference. During controlled metabolic conditions, the cerebral 1H NMR-detected glutamate-to-NAA ratio was ∼ 20–30% lower than expected from the ratio of neutralized perchloric acid extracts of the preparations. Inhibition of respiration in the presence of glucose did not change the 1H NMR glutamate-to-NAA ratio in brain slice preparation. In contrast, either complete depletion of ATP during cyanide poisoning together with 0 m M glucose, anoxia in the absence of glucose, or treatment with nigericin or with a protonophore, carbonyl cyanide- m -fluorophenylhydrazone, increased 1H NMR-detected glutamate/NAA in the cerebral preparations without a change in the relative and absolute concentration ratios determined from the tissue acid extracts. Spin-spin relaxation times of glutamate and NAA peaks in anoxic slices were 749 ± 89 and 729 ± 94 ms, respectively, and thus, the portion of glutamate that could not be detected by 1H NMR was quantified in absolute terms. It was calculated that an increase in the glutamate-to-NAA ratio from 0.55 ± 0.02 to 0.67 ± 0.02 during aglycemic anoxia corresponded to some 6 mmol/kg of tissue dry weight of glutamate from the total concentration of 28 mmol/kg dry weight. It is suggested that this 22% of total glutamate pool is present in a noncytoplasmic compartment during controlled metabolic state.  相似文献   
9.
The needle trace method was used to study retrospectively the long-term latitudinal variation in needle retention in Scots pine (Pinus sylvestris L.) in Finland. The mean annual summer needle retention (ANR) along the main stem varied from 3.4 to 6.0 needle sets during the period 1957–1991. The lowest values were observed in southern and the highest in northern Finland. The length of the growing season, expressed as the thermal sum (threshold value +5 °C), was negatively correlated with the mean ANR (r=-0.96). The geographical needle retention pattern (NRP) for the period 1957–1991 showed a clearly increasing trend from 1957 to 1969 (southern Finland) and to 1975 (northern Finland); thereafter, the NRP tended to decrease close to its minimum value recorded in 1991. The general level of the NRP was approximately 5.0 needle sets in northern Finland and 3.5–4.0 needle sets in southern Finland. The NRP, with its 6–12 year cycle for southern Finland, was clearly periodical. Differences in the NRP among the ten stands in southern Finland were small, whereas the said periodicity was missing and the differences were high among the stands in northern Finland. The results indicate that variation in the number of needle sets, viz. defoliation of pines, is a normal phenomenon. The role of net carbon assimilation as a regulator of the number of needle sets is discussed.  相似文献   
10.
The exotoxin produced by certain serotypes of Bacillus thuringiensis was used as a means of microbiological control of the larval development of flies. The optimal batch cultivation conditions with respect to pH, temperature, aeration, agitation, and initial concentration of growth-limiting substrate were determined. A dynamic model describing the process was designed and fitted to the experimental data. The application of a method for estimating exotoxin and bacterial concentrations from on-line measurable quantities such as oxygen consumption and heat production is presented.  相似文献   
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