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1.
Sheppard PR Helsel DR Speakman RJ Ridenour G Witten ML 《Chemico-biological interactions》2012,196(3):96-101
Previously reported dendrochemical data showed temporal variability in concentration of tungsten (W) and cobalt (Co) in tree rings of Fallon, Nevada, US. Criticism of this work questioned the use of the Mann-Whitney test for determining change in element concentrations. Here, we demonstrate that Mann-Whitney is appropriate for comparing background element concentrations to possibly elevated concentrations in environmental media. Given that Mann-Whitney tests for differences in shapes of distributions, inter-tree variability (e.g., "coefficient of median variation") was calculated for each measured element across trees within subsites and time periods. For W and Co, the metals of highest interest in Fallon, inter-tree variability was always higher within versus outside of Fallon. For calibration purposes, this entire analysis was repeated at a different town, Sweet Home, Oregon, which has a known tungsten-powder facility, and inter-tree variability of W in tree rings confirmed the establishment date of that facility. Mann-Whitney testing of simulated data also confirmed its appropriateness for analysis of data affected by point-source contamination. This research adds important new dimensions to dendrochemistry of point-source contamination by adding analysis of inter-tree variability to analysis of central tendency. Fallon remains distinctive by a temporal increase in W beginning by the mid 1990s and by elevated Co since at least the early 1990s, as well as by high inter-tree variability for W and Co relative to comparison towns. 相似文献
2.
Investigation of germination and aging in Moravian III barley grain by nuclear magnetic resonance. 总被引:2,自引:0,他引:2
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High-resolution, solid-state 1H nuclear magnetic resonance (NMR) techniques are used for the first time to study germination in imbibed Moravian III barley grains. Whereas magic-angle spinning 1H NMR spectra reveal the water and lipid components in barley grains, combined rotation and multiple-pulse spectroscopy techniques provide 1H NMR spectra of grains that reveal the protein and carbohydrate as well as the water and lipid components. Spectra of grains are compared with spectra of model compounds to verify assignments. 1H T1 and T2 measurements using magic-angle spinning only and combined rotation and multiple-pulse spectroscopy techniques provide information about molecular mobility within the grains during inhibition. Some grains were subjected to artificial aging conditions. 1H NMR spectral comparisons are made between normal, viable grains and artificially aged grains. 相似文献
3.
Wei-Ming He Giles C. Thelen Wendy M. Ridenour Ragan M. Callaway 《Biological invasions》2010,12(10):3591-3598
A central hypothesis in ecology is that plant life history evolution is constrained by fundamental “compromises between the
conflicting selection pressures resulting from particular combinations of competition, stress, and disturbance”, with stress
being defined as abiotic conditions that restrict production. Biogeographic differences among native and non-native ranges
of invasive plants may provide unique opportunities for tests of this theory. We conducted a greenhouse experiment with Centaurea stoebe plants from North American and European populations. We compared the total biomass and phenotypic plasticity indices for
plants from the native and non-native ranges under stressed and non-stressed conditions. The average size of Centaurea stoebe plants from 13 North American populations was greater than that of plants from 18 European populations regardless of stress
treatment. However, when plants from the same populations were exposed to lower resources the differences in biomass between
plants from North American and European populations were significantly less, suggesting that large plants were poorer stress
tolerators. For all 31 populations the regression slope for the relationship between mean mass for populations in non-stressful
conditions and mean mass in stressful conditions was less than 1.0, indicating that populations that produced large plants
in good conditions also produced plants that grew disproportionately less in stressful conditions. These findings suggest
that Centaurea stoebe may be evolving towards being a good “competitor” (sensu Grime, 1977) in its invasive range, but at the cost of being a good “stress tolerator”. 相似文献
4.
Cercospora zeae-maydis causes gray leaf spot of maize, which has become one of the most widespread and destructive diseases of maize in the world. C. zeae-maydis infects leaves through stomata, which is predicated on the ability of the pathogen to perceive stomata and reorient growth accordingly. In this study, the discovery that light was required for C. zeae-maydis to perceive stomata and infect leaves led to the identification of CRP1, a gene encoding a putative blue-light photoreceptor homologous to White Collar-1 (WC-1) of Neurospora crassa. Disrupting CRP1 via homologous recombination revealed roles in multiple aspects of pathogenesis, including tropism of hyphae to stomata, the formation of appressoria, conidiation, and the biosynthesis of cercosporin. CRP1 was also required for photoreactivation after lethal doses of UV exposure. Intriguingly, putative orthologs of CRP1 are central regulators of circadian clocks in other filamentous fungi, raising the possibility that C. zeae-maydis uses light as a key environmental input to coordinate pathogenesis with maize photoperiodic responses. This study identified a novel molecular mechanism underlying stomatal tropism in a foliar fungal pathogen, provides specific insight into how light regulates pathogenesis in C. zeae-maydis, and establishes a genetic framework for the molecular dissection of infection via stomata and the integration of host and pathogen responses to photoperiod. 相似文献
5.
Lee YT Dimitrova YN Schneider G Ridenour WB Bhattacharya S Soss SE Caprioli RM Filipek A Chazin WJ 《Biochemistry》2008,47(41):10921-10932
S100A6 is a member of the S100 subfamily of EF-hand Ca (2+) binding proteins that has been shown to interact with calcyclin binding protein/Siah-1 interacting protein (CacyBP/SIP or SIP), a subunit of an SCF-like E3 ubiquitin ligase complex (SCF-TBL1) formed under genotoxic stress. SIP serves as a scaffold in this complex, linking the E2-recruiting module Siah-1 to the substrate-recruiting module Skp1-TBL1. A cell-based functional assay suggests that S100A6 modulates the activity of SCF-TBL1. The results from the cell-based experiments could be enhanced if it were possible to selectively inhibit S100A6-SIP interactions without perturbing any other functions of the two proteins. To this end, the structure of the S100A6-SIP complex was determined in solution by NMR and the strength of the interaction was characterized by isothermal titration calorimetry. In an initial step, the minimal S100A6 binding region in SIP was mapped to a 31-residue fragment (Ser189-Arg219) in the C-terminal domain. The structure of the S100A6-SIP(189-219) complex revealed that SIP(189-219) forms two helices, the first of which (Met193-Tyr200) interacts with S100A6 in a canonical binding mode. The second helix (Met207-Val216) lies over the S100A6 dimer interface, a mode of binding to S100A6 that has not previously been observed for any target bound to an S100 protein. A series of structure-based SIP mutations showed reduced S100A6 binding affinity, setting the stage for direct functional analysis of S100A6-SIP interactions. 相似文献
6.
Weir TL Bais HP Stull VJ Callaway RM Thelen GC Ridenour WM Bhamidi S Stermitz FR Vivanco JM 《Planta》2006,223(4):785-795
Centaurea maculosa Lam. is a noxious weed in western North America that produces a phytotoxin, (±)-catechin, which is thought to contribute to its invasiveness. Areas invaded by C. maculosa often result in monocultures of the weed, however; in some areas, North American natives stand their ground against C. maculosa and show varying degrees of resistance to its phytotoxin. Two of these resistant native species, Lupinus sericeus Pursh and Gaillardia grandiflora Van Houtte, were found to secrete increased amounts of oxalate in response to catechin exposure. Mechanistically, we found that oxalate works exogenously by blocking generation of reactive oxygen species in susceptible plants and reducing oxidative damage generated in response to catechin. Furthermore, field experiments show that L. sericeus indirectly facilitates native grasses in grasslands invaded by C. maculosa, and this facilitation can be correlated with the presence of oxalate in soil. Addition of exogenous oxalate to native grasses and Arabidopsis thaliana (L.) Heynh grown in vitro alleviated the phytotoxic effects of catechin, supporting the field experiments and suggesting that root-secreted oxalate may also act as a chemical facilitator for plant species that do not secrete the compound. 相似文献
7.
Facilitative plant interactions and climate simultaneously drive alpine plant diversity 总被引:1,自引:0,他引:1
Lohengrin A. Cavieres Rob W. Brooker Bradley J. Butterfield Bradley J. Cook Zaal Kikvidze Christopher J. Lortie Richard Michalet Francisco I. Pugnaire Christian Schöb Sa Xiao Fabien Anthelme Robert G. Björk Katharine J. M. Dickinson Brittany H. Cranston Rosario Gavilán Alba Gutiérrez‐Girón Robert Kanka Jean‐Paul Maalouf Alan F. Mark Jalil Noroozi Rabindra Parajuli Gareth K. Phoenix Anya M. Reid Wendy M. Ridenour Christian Rixen Sonja Wipf Liang Zhao Adrián Escudero Benjamin F. Zaitchik Emanuele Lingua Erik T. Aschehoug Ragan M. Callaway 《Ecology letters》2014,17(2):193-202
Interactions among species determine local‐scale diversity, but local interactions are thought to have minor effects at larger scales. However, quantitative comparisons of the importance of biotic interactions relative to other drivers are rarely made at larger scales. Using a data set spanning 78 sites and five continents, we assessed the relative importance of biotic interactions and climate in determining plant diversity in alpine ecosystems dominated by nurse‐plant cushion species. Climate variables related with water balance showed the highest correlation with richness at the global scale. Strikingly, although the effect of cushion species on diversity was lower than that of climate, its contribution was still substantial. In particular, cushion species enhanced species richness more in systems with inherently impoverished local diversity. Nurse species appear to act as a ‘safety net’ sustaining diversity under harsh conditions, demonstrating that climate and species interactions should be integrated when predicting future biodiversity effects of climate change. 相似文献
8.
Wei-Ming He Yulong Feng Wendy M. Ridenour Giles C. Thelen Jarrod L. Pollock Alecu Diaconu Ragan M. Callaway 《Oecologia》2009,159(4):803-815
Recent studies suggest that the invasive success of Centaurea maculosa may be related to its stronger allelopathic effects on native North American species than on related European species, one
component of the “novel weapons” hypothesis. Other research indicates that C. maculosa plants from the invasive range in North America have evolved to be larger and better competitors than conspecifics from the
native range in Europe, a component of the “evolution of increased competitive ability” hypothesis. These hypotheses are not
mutually exclusive, but this evidence sets the stage for comparing the relative importance of evolved competitive ability
to inherent competitive traits. In a competition experiment with a large number of C. maculosa populations, we found no difference in the competitive effects of C. maculosa plants from North America and Europe on other species. However, both North American and European C. maculosa were much better competitors against plants native to North America than congeners native to Romania, collected in areas
where C. maculosa is also native. These results are consistent with the novel weapons hypothesis. But, in a second experiment using just one
population from North America and Europe, and where North American and European species were collected from a broader range
of sites, competitive interactions were weaker overall, and the competitive effects of C. maculosa were slightly stronger against European species than against North American species. Also consistent with the novel weapons
hypothesis, (±)-catechin had stronger effects on native North American species than on native European species in two experiments.
Our results suggest that the regional composition of the plant communities being invaded by C. maculosa may be more important for invasive success than the evolution of increased size and competitive ability.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
9.
B. J. Butterfield L. A. Cavieres R. M. Callaway B. J. Cook Z. Kikvidze C. J. Lortie R. Michalet F. I. Pugnaire C. Schb S. Xiao B. Zaitchek F. Anthelme R. G. Bjrk K. Dickinson R. Gaviln R. Kanka J.‐P. Maalouf J. Noroozi R. Parajuli G. K. Phoenix A. Reid W. Ridenour C. Rixen S. Wipf L. Zhao R. W. Brooker 《Ecology letters》2013,16(4):478-486
Biotic interactions can shape phylogenetic community structure (PCS). However, we do not know how the asymmetric effects of foundation species on communities extend to effects on PCS. We assessed PCS of alpine plant communities around the world, both within cushion plant foundation species and adjacent open ground, and compared the effects of foundation species and climate on alpha (within‐microsite), beta (between open and cushion) and gamma (open and cushion combined) PCS. In the open, alpha PCS shifted from highly related to distantly related with increasing potential productivity. However, we found no relationship between gamma PCS and climate, due to divergence in phylogenetic composition between cushion and open sub‐communities in severe environments, as demonstrated by increasing phylo‐beta diversity. Thus, foundation species functioned as micro‐refugia by facilitating less stress‐tolerant lineages in severe environments, erasing a global productivity – phylogenetic diversity relationship that would go undetected without accounting for this important biotic interaction. 相似文献
10.
Adam Johnson Li-Mei Chen Emily Winne Wanda Santana Maureen G. Metcalfe Guaniri Mateu-Petit Callie Ridenour M. Jaber Hossain Julie Villanueva Sherif R. Zaki Tracie L. Williams Nancy J. Cox John R. Barr Ruben O. Donis 《PloS one》2015,10(6)
One of the important lessons learned from the 2009 H1N1 pandemic is that a high yield influenza vaccine virus is essential for efficient and timely production of pandemic vaccines in eggs. The current seasonal and pre-pandemic vaccine viruses are generated either by classical reassortment or reverse genetics. Both approaches utilize a high growth virus, generally A/Puerto Rico/8/1934 (PR8), as the donor of all or most of the internal genes, and the wild type virus recommended for inclusion in the vaccine to contribute the hemagglutinin (HA) and neuraminidase (NA) genes encoding the surface glycoproteins. As a result of extensive adaptation through sequential egg passaging, PR8 viruses with different gene sequences and high growth properties have been selected at different laboratories in past decades. The effect of these related but distinct internal PR8 genes on the growth of vaccine viruses in eggs has not been examined previously. Here, we use reverse genetics to analyze systematically the growth and HA antigen yield of reassortant viruses with 3 different PR8 backbones. A panel of 9 different HA/NA gene pairs in combination with each of the 3 different lineages of PR8 internal genes (27 reassortant viruses) was generated to evaluate their performance. Virus and HA yield assays showed that the PR8 internal genes influence HA yields in most subtypes. Although no single PR8 internal gene set outperformed the others in all candidate vaccine viruses, a combination of specific PR8 backbone with individual HA/NA pairs demonstrated improved HA yield and consequently the speed of vaccine production. These findings may be important both for production of seasonal vaccines and for a rapid global vaccine response during a pandemic. 相似文献