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Sasha Larsen Jun Weaver Katherine de Sa Campos Rhobe Bulahan Jackson Nguyen Heather Grove Amy Huang Lauren Low Namphuong Tran Seth Gomez Jennifer Yau Thomas Ilustrisimo Jessica Kawilarang Jonathan Lau Maivi Tranphung Irene Chen Christina Tran Marcia Fox Joan Lin-Cereghino Geoff P. Lin-Cereghino 《Biotechnology letters》2013,35(11):1925-1935
Although Pichia pastoris is a popular protein expression system, it exhibits limitations in its ability to secrete heterologous proteins. Therefore, a REMI (restriction enzyme mediated insertion) strategy was utilized to select mutant beta-g alactosidase s upersecretion (bgs) strains that secreted increased levels of a β-galactosidase reporter. Many of the twelve BGS genes may have functions in intracellular signaling or vesicle transport. Several of these strains also appeared to contain a more permeable cell wall. Preliminary characterization of four bgs mutants showed that they differed in the ability to enhance the export of other reporter proteins. bgs13, which has a disruption in a gene homologous to Saccharomyces cerevisiae protein kinase C (PKC1), gave enhanced secretion of most recombinant proteins that were tested, raising the possibility that it has the universal super-secreter phenotype needed in an industrial production strain of P. pastoris. 相似文献
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