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1.
The aims of this study were to investigate the control of fatty acid synthesis and its relationship to surfactant production in the fetal lung during alteration of hormonal and substrate conditions. Lung explants from 18 day fetuses (term = 22 days) which were cultured 2 days in the presence of 10 mM lactate showed parallel acceleration of de novo fatty acid synthesis (3H2O incorporation) and [14C]choline incorporation into disaturated phosphatidylcholine (DSPC) compared to culture of explants in glucose. Both the cultured and fresh explants were resistant to the classical short term (4 h) cAMP inhibition of fatty acid synthesis with 3 mM dibutyryl cAMP or 0.5 mM aminophylline. In the cultured explants short term cAMP elevation increased DSPC production, and long term (2 day) cAMP elevation caused a further increase in DSPC synthesis and also stimulated fatty acid synthesis. In cultured explants from 17 day fetuses, dexamethasone (0.1 microM) caused a synergistic increase with aminophylline in both fatty acid synthesis and DSPC production whereas, in explants from 18 day fetuses, dexamethasone inhibited both processes and reduced the level of stimulation of DSPC and fatty acid synthesis seen with aminophylline alone. Dexamethasone also reduced the stimulation of both DSPC and fatty acid synthesis produced in the culture of 18 day explants with bacitracin (0.5 mg/ml), whereas the combination of bacitracin and aminophylline resulted in a synergistic increase in DSPC production. Culture with glucagon (0.1 microM) also stimulated DSPC synthesis but at physiological levels insulin had no effect on either DSPC or fatty acid synthesis. These data show that lung fatty acid synthesis exhibits unique features of fatty acid synthesis regulation compared to other lipogenic tissues and also suggest a link between de novo fatty acid synthesis and surfactant production during the critical period of accelerated lung maturation. 相似文献
2.
Biochemical pathways in prokaryotes can be traced backward through evolutionary time 总被引:10,自引:0,他引:10
For the first time, a credible prokaryotic phylogenetic tree is being
assembled by Woese and others using quantitative sequence analysis of
oligonucleotides in the highly conservative rRNA. This provides an
evolutionary scale against which the evolutionary steps that led to the
arrangement and regulation of contemporary biochemical pathways can be
measured. This paper presents an emerging evolutionary picture of aromatic
amino acid biosynthesis within a large superfamily assemblage of
prokaryotes that is sufficiently developed to illustrate a new perspective
that will be applicable to many other biochemical pathways.
相似文献
3.
The serologic types of 265 isolates of Pasteurella multocida collected from 50 species of wild birds and seven species of wild mammals over a 22-yr period were determined with the gel diffusion precipitin test. Antigens prepared from these isolates reacted with reference P. multocida antisera representing serotypes 1, 2, 3, 4, 5, 6, 7, 8, 12, and 15. Antigens from some isolates reacted slightly with antisera from more than one serotype. Overall, gel precipitin reactions involving serotype 1 (65%) and 3 (20%) were the most prevalent. 相似文献
4.
H J Enfiejian N L Chiaia G J Macdonald R W Rhoades 《Somatosensory & motor research》1989,6(5-6):537-552
Neonatal transection results in a marked reduction of the number of trigeminal (V) ganglion cells that contribute axons to the regenerate infraorbital nerve (ION; Jacquin and Rhoades, 1985; Chiaia et al., 1987). Such lesions also produce a profound deafferentation of the V brain stem complex that appears to spare the innervation of layers I and II of subnucleus caudalis (SpC) by substance-P-positive (SP-positive) primary afferents (Jacquin and Rhoades, 1985; Rhoades et al., 1988). In the present study, we combined retrograde tracing with immunocytochemistry to determine whether neonatal transection of the ION alters the percentage of SP-positive V ganglion cells that contribute axons to this V branch upon regeneration. In V ganglia ipsilateral to the intact ION (n = 8), 11.6% +/- 3.2% of the cells labeled after application of true blue (TB) to the ION were also SP-positive. In ganglia ipsilateral to the neonatally damaged nerve (n = 8), 18.6% +/- 4.7% of the cells labeled after application of TB to the regenerate ION were also SP-positive (p less than 0.001). We also compared the SP content of intact ganglia (n = 10) with that of ganglia ipsilateral to the damaged nerve (n = 10) by means of radioimmunoassay. The normal V ganglia contained (mean +/- SD) 3496 +/- 774 pg SP/mg protein. The value for the ganglia ipsilateral to the damaged nerve was 5533 +/- 1746 pg SP/mg protein (p less than 0.01). There was no significant difference between SP levels on the control and partially deafferented sides of the brain stem in neonatally nerve-damaged adult rats. In one additional experiment, we injected TB into both vibrissa pads of seven rats on the day of birth prior to transection of the ION. After an 8-hr delay, the nerve on one side was then cut and allowed to regenerate, and both V ganglia were then processed for immunocytochemistry. On the nerve-damage side, 25.8% of the TB-labeled cells were SP-positive. The value for the intact side was 12.0% (p less than 0.000001). This result demonstrated that the lesion-induced change in the percentage of SP-positive ION cells was not the result of either late-growing axons from SP-positive ganglion cells that may have been missed by our nerve cuts or collateral sprouting into the regenerate ION by undamaged SP-positive ganglion cells. 相似文献
5.
IL-4 down-regulates IL-1 and TNF gene expression in human monocytes 总被引:37,自引:0,他引:37
R Essner K Rhoades W H McBride D L Morton J S Economou 《Journal of immunology (Baltimore, Md. : 1950)》1989,142(11):3857-3861
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8.
R A Rhoades 《Journal of applied physiology》1975,38(3):513-516
The relative utilization of [U-14C]glucose and [1-14C]palmitate was examined in lung slices of male Long Evans hooded rats fed ad libitum and starved for 72 h. Food deprivation (72-h fast) significantly decreased [U-14C]flucose oxidation and incorporation into lung lipids. Glucose incorporation into phospholipid-fatty acid (53%) was, in proportion, more markedly reduced than into phospholipid-gluceride glycerol (33%), suggesting that glucose was being conserved for the formation of alpha-glycerol phosphate. (1-14C) palmitate utilization following fasting showed a significant 40% increase in oxidation, and a significant 16% increase in phospholipids, indicating preferential utilization of fatty acids over glucose. Phospholipid fatty acid composition, surface tension measurements and volume-pressure curves were not affected by fasting. Khe data indicate that glucose and palmitate metabolism are interrelated, and that the relative utilization of these substrates is changed to maintain essential lung lipids during an altered physiologic state. 相似文献
9.
MARIA JOSÉ ASINS IRENE VILLALTA MOHAMED M. ALY RAQUEL OLÍAS PAZ ÁLVAREZ DE MORALES RAÚL HUERTAS JUN LI NOELIA JAIME‐PÉREZ ROSARIO HARO VERÓNICA RAGA EMILIO A. CARBONELL ANDRÉS BELVER 《Plant, cell & environment》2013,36(6):1171-1191
The location of major quantitative trait loci (QTL) contributing to stem and leaf [Na+] and [K+] was previously reported in chromosome 7 using two connected populations of recombinant inbred lines (RILs) of tomato. HKT1;1 and HKT1;2, two tomato Na+‐selective class I‐HKT transporters, were found to be closely linked, where the maximum logarithm of odds (LOD) score for these QTLs located. When a chromosome 7 linkage map based on 278 single‐nucleotide polymorphisms (SNPs) was used, the maximum LOD score position was only 35 kb from HKT1;1 and HKT1;2. Their expression patterns and phenotypic effects were further investigated in two near‐isogenic lines (NILs): 157‐14 (double homozygote for the cheesmaniae alleles) and 157‐17 (double homozygote for the lycopersicum alleles). The expression pattern for the HKT1;1 and HKT1;2 alleles was complex, possibly because of differences in their promoter sequences. High salinity had very little effect on root dry and fresh weight and consequently on the plant dry weight of NIL 157‐14 in comparison with 157‐17. A significant difference between NILs was also found for [K+] and the [Na+]/[K+] ratio in leaf and stem but not for [Na+] arising a disagreement with the corresponding RIL population. Their association with leaf [Na+] and salt tolerance in tomato is also discussed. 相似文献
10.
华南丘陵地区农林复合生态系统晚稻田甲烷和氧化亚氮排放 总被引:5,自引:0,他引:5
采用静态箱-气相色谱法对晚稻田甲烷(CH4)和氧化亚氮(N2O)排放进行田间原位测定。结果表明,有植株参与的稻田CH4排放通量季节变化与地下5cm温度呈显著正相关关系。稻田CH4和N2O季节平均排放通量在有植株参与时分别为1.16±0.38mgm-2h-1和42.33±20.00μgm-2h-1,而无植株参与的分别为0.15±0.11mgm-2h-1和51.69±15.87μgm-2h-1。水稻种植对CH4的排放影响较大,对N2O的排放影响较小,有植株参与的稻田CH4平均排放量显著高于无植株参与的稻田,N2O的平均排放量无显著差异。 相似文献