排序方式: 共有4条查询结果,搜索用时 46 毫秒
1
1.
All life forms are equipped with rapidly acting, evolutionally conserved components of an innate immune defense system that consists of a group of unique and diverse molecules known as host defense peptides (HDPs). A Systematic and Modular Modification and Deletion (SMMD) approach was followed to analyse the structural requirement of B1CTcu5, a brevinin antibacterial peptide amide identified from the skin secretion of frog Clinotarsus curtipes, India, to show antibacterial activity and to explore the active core region. Seventeen SMMD-B1CTcu5 analogs were designed and synthesised by C and N-terminal amino acid substitution or deletion. Enhancement in cationicity by N-terminal Lys/Arg substitution or hydrophobicity by Trp substitution produced no drastic change in bactericidal nature against selected bacterial strains except S. aureus. But the sequential removal of N-terminal amino acids had a negative effect on bactericidal potency. Analog B1CTcu5-LIAG obtained by the removal of four N-terminal amino acids displayed bactericidal effect comparable to, or in excess of, the parent peptide with reduced hemolytic character. Its higher activity was well correlated with the improved inner membrane permeabilisation capacity. This region may act as the active core of B1CTcu5. Presence of C-terminal disulphide bond was not a necessary condition to display antibacterial activity but helped to promote hemolytic nature. Removal of the C-terminal rana box region drastically reduced antibacterial and hemolytic activity of the peptide, showing that this region is important for membrane targeting. The bactericidal potency of the D-peptide (DB1CTcu5) helped to rule out the stereospecific interaction with the bacterial membrane. Our data suggests that both the C and N-terminal regions are necessary for bactericidal activity, even though the active core region is located near the N-terminal of B1CTcu5. A judicious modification at the N-terminal region may produce a short SMMD analog with enhanced bactericidal activity and low toxicity against eukaryotic cells. 相似文献
2.
Subramanium Vimaladevi Ayyavu Mahesh Balaji N. Dhayanithi Nattarayan Karthikeyan 《Biologia》2012,67(1):212-216
Mosquito larvicidal and repellent activities of phenolic acids of Chaetomorpha antennina (Bory) Kuetz. against the third instar larvae of Aedes aegypti were investigated. The larval mortality was observed after 24 h exposure. Results of mosquito larvicidal tests revealed that
insoluble bound phenolic acids and soluble conjugated phenolic acid fractions of C. antennina had an excellent inhibitory
effect against A. aegypti and its LC50 values were 23.4 and 44.6 μg ml−1, respectively. The repellency assay of insoluble bound phenolic acids and soluble conjugated phenolic acid fractions of C. antennina, at 10 μg cm−2 concentration gave 100% protection up to 120 min. The results indicate that phenolic acids of C. antennina have a wide spectrum of larvicidal and repellent activities against Aedes aegypti. 相似文献
3.
Hylarana is a well established frog genus coming under the family Ranidae. An increasing number of antimicrobial peptides have been
isolated and characterized from the skin of frogs of this genus. This review covers the antimicrobial peptides reported so
far from the frogs of Hylarana genus and to propose a consistent system of nomenclature for amphibian skin peptides. Multiple sequence alignment of the
skin peptides from Hylarana genus has grouped them into six peptide families, and three bioactive peptides. Existing nomenclature of amphibian antimicrobial
peptides is species centered with no implication to the genus which can lead to disparities, when frogs with same species
name belonging to different genus have to be named. As per the proposed system the peptide should have the parent peptide
name (e.g. Brevinin-1) followed by two uppercase letter of the genus, if two genera begin with the same letter–first letter
should be the same followed by an appropriate second letter (e.g. HU for Huia and HM for Humenerana). This is succeeded by species name in lower case-orthologous peptides from different species may be characterized by the
initial letter of that species, when two species begin with the same initial letter, second letter should be used appropriately
(e.g. HLat for Hylarana aurata and HLan for Hylarana aurantiaca). Paralogs belonging to the same peptide family are assigned by numbers. 相似文献
4.
Characterization of two novel antimicrobial peptides from the cuticular extracts of the ant Trichomyrmex criniceps (Mayr), (Hymenoptera: Formicidae)
下载免费PDF全文
![点击此处可从《Archives of insect biochemistry and physiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Nagachaitanya Bhagavathula Venkateshwarlu Meedidoddi Simon Bourque Reshmy Vimaladevi Santoshkumar Kesavakurup Dayanandan Selvadurai Sameer Shrivastava Chandrashekara Krishnappa 《Archives of insect biochemistry and physiology》2017,94(4)
Antimicrobial peptides (AMPs) from cuticular extracts of worker ants of Trichomyrmex criniceps (Mayr, Hymenoptera: Formicidae) were isolated and evaluated for their antimicrobial activity. Eight peptides ranging in mass from 804.42 to 1541.04 Da were characterized using a combination of analytical and bioinformatics approach. All the eight peptides were novel with no similarity to any of the AMPs archived in the Antimicrobial Peptide Database. Two of the eight novel peptides, the smallest and the largest by mass were named Crinicepsin‐1 and Crinicepsin‐2 and were chemically synthesized by solid phase peptide synthesis. The two synthetic peptides had antibacterial and weak hemolytic activity. 相似文献
1