不同病理分期肺腺癌患者血清代谢组学研究

肺癌是当今世界最常见的恶性肿瘤之一,其新发率和死亡率多年来都居于各类癌症之首,其中85%的肺癌都是非小细胞癌,而腺癌又是最常见的非小细胞癌。肺癌的高隐匿性是造成其高死亡率的最主要原因,因此为肺癌的早期诊断和病理分期寻求高效可靠的方法是十分必要的。代谢组学揭示了小分子代谢物的一系列变化,反映了生命活动的最终状态,因此也能直接反映疾病不同发展阶段的病理生理变化。本研究利用核磁共振氢谱(1H-NMR),对在我院就诊的27例不同病理分期的肺腺癌患者和13例健康志愿者进行了血清代谢物分析,运用正交偏最小二乘判别分析(OPLS-DA)对1H-NMR数据进行建模,单变量统计分析对模型进行评价。结果表明肺腺癌患者组的血清中有14种代谢物出现明显差异,其中丙酮酸、丙氨酸、NAC1、乳酸、GPC和甘氨酸比起对照组来有显著上升,而葡萄糖、谷氨酰胺、亮氨酸、异亮氨酸、缬氨酸、丙酮、乙酰乙酸和苏氨酸则显著下降。而在不同分期肺腺癌患者间进行比较后发现,异亮氨酸、乙酰乙酸、NAC1和乳酸的变化与肺腺癌的发展有相关性,可能是肺腺癌早期诊断和分期的潜在生物标志物。     

Biomimetic injectable HUVEC‐adipocytes/collagen/alginate microsphere co‐cultures for adipose tissue engineering

Engineering adipose tissue that has the ability to engraft and establish a vascular supply is a laudable goal that has broad clinical relevance, particularly for tissue reconstruction. In this article, we developed novel microtissues from surface‐coated adipocyte/collagen/alginate microspheres and human umbilical vein endothelial cells (HUVECs) co‐cultures that resembled the components and structure of natural adipose tissue. Firstly, collagen/alginate hydrogel microspheres embedded with viable adipocytes were obtained to mimic fat lobules. Secondly, collagen fibrils were allowed to self‐assemble on the surface of the microspheres to mimic collagen fibrils surrounding the fat lobules in the natural adipose tissue and facilitate HUVEC attachment and co‐cultures formation. Thirdly, the channels formed by the gap among the microspheres served as the room for in vitro prevascularization and in vivo blood vessel development. The endothelial cell layer outside the microspheres was a starting point of rapid vascular ingrowth. Adipose tissue formation was analyzed for 12 weeks at 4‐week intervals by subcutaneous injection into the head of node mice. The vasculature in the regenerated tissue showed functional anastomosis with host blood vessels. Long‐term stability of volume and weight of the injection was observed, indicating that the vasculature formed within the constructs benefited the formation, maturity, and maintenance of adipose tissue. This study provides a microsurgical method for adipose regeneration and construction of biomimetic model for drug screening studies. Biotechnol. Bioeng. 2013; 110: 1430–1443. © 2012 Wiley Periodicals, Inc.     

Proton Nuclear Magnetic Resonance Studies on Huwentoxin-I from the Venom of the Spider Selenocosmia huwena: 2. Three-Dimensional Structure in Solution

The three-dimensional structure in aqueous solution of native huwentoxin-I, a neurotoxin from the venom of the spider Selenocosmia huwena, has been determined from two-dimensional ¹H NMR data recorded at 500 and 600 MHz. Structural constraints consisting of interproton distances inferred from NOEs and dihedral angles from spin–spin coupling constants were used as input for distance geometry calculation with the program XPLOR 3.1. The best 10 structures have NOE violations <0.3 Å, dihedral violations <2°, and pairwise root-mean-square differences of 1.08 (±0.20) Å over backbone atoms (N, C;, C). The molecule adopts a compact structure consisting of a small triple-stranded antiparallel -sheet and five -turns. A small hydrophobic patch consisting of Phe 6, Trp 28, and Trp 31 is located on one side of the molecule. All six lysine residues are distributed on the molecular surface. The three disulfidc bridges are buried within the molecule. The structure contains an inhibitor cystine knot motif which is adopted by several other small proteins, such as -conotoxin, agatoxin IVA, and gurmarin.     

折翼蝠冬眠期几项生理常数的测定及分析

折翼蝠(Miniopterus schreibersii chinensis Thomas)在皖南地区数量较多、分布较广,是哺乳类中具冬眠习性的异温动物。这种异温动物的生理生态研究,国内极少报道。我们于1981年10月至1982年5月对折翼蝠冬眠期的几项生理常数做了测定和分析,目的在于了解它们在冬眠期间的生理变化及其与外界环境因子之间的相互关系,从而进一步探讨异温动物冬眠期的代谢规律,为休眠的理论和动物资源的利用提供一点参考资料。     

皖南地区翼手类初步研究

皖南地处江南,约介于东经116°37′-119°31′,北纬29°24′-31°10′,境内沿江及北部为冲积平原,南部为山区,天然洞穴或开山采石的废弃坑道等为翼手类提供了适宜的栖息环境。     

甜橙叶片营养元素适宜含量的研究

本研究探讨闽南甜橙主栽品种(印子柑、改良橙、伏令夏橙)叶片营养元素含量的适宜范围。结果表明,各品种不同地点、年份的叶片元素含量存在明显差异,而三个甜橙品种间叶片元素含量未见稳定的差异,因此,作者认为,可建立三个甜橙品种通用的叶片营养元素含量适宜标准。统计分析表明:其叶片毛素含量的变异系数,大量元素以氮、磷较小,镁、钾、钙较大;微量元素多高于大量元素。本文初步提出甜橙于片元素的适宜含量:氮2.50％～3.30％,磷0.12％～0.18％,钾1.00％～2.00％,钙2.00％～3.50％,镁0.22％～0.40％,铜4.0～18.0ppm,锌25.0～70.0ppm,锰20.0～100.0ppm,铁90.0～160.0ppm,硼25.0～100.0ppm。大量元素含量的适宜比值为氮:磷:钾:钙:镁=1:0.05:0.5:2:0.95:0.11。上列指标可供甜橙营养诊断指导合理施肥之参考。     

成年龙眼园平衡施肥示范试验

示范试验结果表明,成年龙眼的平衡施肥对提高土壤肥力、保持树体适宜营养水平以及增加果实产量有明显作用,从而证实平衡施肥方案的可行性。作者推荐,闽南丘陵地成年龙眼园的年施肥方案（667m2,产果1吨）：N20～25kg（有机肥的N约占年施N量的40％）;N：P2O5：K2O：CaO：MgO=1．0：0．5～0．6：1．0～1．1：0．8：0．4。     

绒山蝠生态的初步调查

绒山蝠(Nyctalus noctula velutinus)是芜湖市周围地区常见的食虫蝙蝠,国内仅见于南方。关于翼手类的生态资料,国内报道不多,绒山蝠的生态尚未见系统报告。从1980年起,我们连续3年对绒山蝠进行了定点观察和测定,现将初步调查报道如下。     

Rapid prototyping as a tool for manufacturing bioartificial livers

Rapid prototyping (RP) technologies are a set of manufacturing processes that can produce very complex structures directly from computer-aided design models without structure-specific tools or knowledge. These technologies might eventually enable the manufacture of human livers to create functional substitutes for treating liver failure or dysfunctionality. However, the approaches used currently face many challenges, such as the complex branched vascular and bile ductular systems and the variety of cell types, matrices and regulatory factors involved in liver development. Here, we discuss the challenges and provide evidence for the usefulness of RP in overcoming them.     

Proton nuclear magnetic resonance studies on huwentoxin-I from the venom of the spiderSelenocosmia huwena: 1. Sequence-specific1H-NMR assignments

The complete sequence-specific assignments of resonances in the¹H-NMR spectrum of huwentoxin-I from the Chinese bird spider,Selenocosmia huwena, is described. A combination of two-dimensional NMR experiments including 2D-COSY, 2D-NOESY, and 2D-TOCSY has been employed on samples of the toxin dissolved in D₂O and in H₂O for assignment purposes. Protons belonging to spin systems for each of the 33 amino acids were identified. The sequence-specific assignments were facilitated by the identification ofd _αN connectivities on the fingerprint regions of the COSY and NOESY spectra and were supported by the identification ofd _NN andd _αN connectivities in the TOCSY and NOESY spectra. These studies provide a basis for the determination of the solution-phase conformation of this toxin.