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James M. Bowen Relda Cailleau Beppino C. Giovanella Sen Pathak Michael J. Siciliano 《In vitro cellular & developmental biology. Plant》1983,19(8):635-641
Summary A transplantable tumor was established in NIH/Swiss/Nu mice from tissue derived from a human breast adenocarcinoma metastatic
to the brain. Cultivation of dispersed cells from the third transplant generation of the tumor produced a rapidly growing,
high-density culture of fibroblastlike cells. Chromosome and isozyme assays showed these cells to be of mouse origin. The
cells behaved as an established line from initial culture. Cells of the tissue culture line, designated NM-1, produced rapidly
growing fibrohistiocytomas in nude mice. Electron microscopy revealed that the cells produced large numbers of type C virus
particles. Serological, biochemical, and infectivity assays indicated that the retrovirus produced by NM-1 cells is an ecotropic,
infective, murine retrovirus antigenically related to, but distinguishable from, Gross and Moloney viruses. The virus did
not transform mouse fibroblasts. The data support the conclusion that mouse stromal cells within the transplanted human tumor
had undergone malignant transformation and induction to virus replication. The role of the virus in the malignant transformation
remains to be clarified. 相似文献
2.
Charles Wiseman Relda Cailleau Matilde Olivé George R. Blumenschein James M. Bowen 《In vitro cellular & developmental biology. Plant》1980,16(7):629-633
Summary Indirect immunofluorescence tests were performed on 14 established human breast cancer cell lines using sera from a variety
of subjects. Autologous reactions were studied on 10 cell lines, with positive reactions demonstrable in 8. Tests using sera
from a randomly selected population of breast cancer patients showed reactivity in 40 to 66% depending on the target cell
line used. Reactivity to other nonbreast cancer cell lines was rare. Several control populations were tested, including normal
blood bank donors, persons with benign breast disease, and persons with other forms of cancer; immunofluorescent antibody
was detected much less frequently in sera from these populations than those from the breast cancer group. Positive reactions
remained in spite of absorption of serum with heterophile antigens, normal human breast tissue, and AB+ red blood cells. Thus
established cell lines of human breast cancer possess antigens commonly recognized by sera from breast cancer patients.
This work was supported in part by Grant CA 16789 from the National Cancer Institute, NIH, Department of Health, Education
and Welfare. 相似文献
3.
Roberto L. Ceriani Jerry A. Peterson Edward W. Blank Curtis M. Chan Relda Cailleau 《In vitro cellular & developmental biology. Animal》1992,28(6):397-402
Summary To establish a model system for preclinical radioimmunotherapy studies, attempts were made to graft 16 different human breast
carcinoma cell lines into BALB/c nu/nu (nude) mice. Nine produced serially transplantable tumors growing at a variable rate,
whereas seven failed to do so. Conversely, three new cell lines were established in monolayer culture from transplantable
human breast tumors in nude mice. Twelve selected tumors and their corresponding cell lines were characterized for DNA ploidy,
% S-phase, and breast epithelial mucin expression by immunohistochemistry and flow cytometry. A wide diversity of these cellular
characteristics were found in that each tumor was unique and distinct from the others. DNA ploidy differed among the tumors
but was not affected by switching between in vitro to in vivo growth. Some tumors expressed similar levels of the breast mucin
both in vitro and in vivo, whereas most expressed lower levels as transplantable tumors. There was a good correlation between
immunohistochemical and flow cytometric determination of surface and cytoplasmic mucin expression, and with both techniques
estrogen and progesterone receptor positive tumors had significantly higher levels of mucin expression than receptor negative
tumors. These 12 transplantable breast tumors, with their corresponding cell lines, provide an excellent model system for
testing radioimmunotherapy and other therapeutic reagents because they exhibit diverse phenotypic characteristics that represented
a mini-population of breast cancer patients’ tumors, allowing assessment of the effect of therapy when confronted with different
breast tumors’ genotype and phenotype. 相似文献
4.
Long-term human breast carcinoma cell lines of metastatic origin: Preliminary characterization 总被引:19,自引:0,他引:19
Cailleau Relda Olivé Matilde Cruciger Quita V. J. 《In vitro cellular & developmental biology. Plant》1978,14(11):911-915
Summary Nineteen human breast carcinoma cell lines have been established as continous cultures during the past 6 years in our laboratory.
This preliminary report is designed to list the lines by their designated code numbers (MDA-MB) and present a brief summary
of their morphological, cytogenetic and biochemical characteristics. Sixteen of our lines were obtained from pleural effusions,
two from brain metastases, and one from pericardial fluid. All lines have been shown to be distinct entities and are uncontaminated
by HeLa cells or each other. A lq marker chromosome is present in all but one of the lines examined.
This research was sponsored by the National Cancer Institute Contract NO1-CB-23869; Institutional Grant 5S 07 RR 5511-15 awarded
by the Division of Research Resources, and a Kelsey-Leary Grant NO 974. 相似文献
5.
James W. Davis Matilde Olive Relda Cailleau James M. Bowen 《Cancer immunology, immunotherapy : CII》1981,11(3):225-231
Summary The antibody reactivity of human breast cancer sera was evaluated by means of radioimmunoassays and established breast cancer cell lines. When tested against the MDA-MB 231 cell line, 30 of 324 sera had detectable antibody reactivity. All the positive sera, however, reacted with other cell lines as well, generally including cultures initiated from sites other than breast cancers, and often including animal cell cultures. In competition radioimmunoassays the positive sera fell into various groups, indicating that a diversity of antigens was being detected. Two patients' sera identified antigens that were expressed on breast cancer cells but that were not expressed on an assortment of other cell types. Sera like these two, which identify potentially important tumor markers, could serve as valuable reagents for the analysis of the tumor-assiciated antigens of human breast cancer cells. 相似文献
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