排序方式: 共有37条查询结果,搜索用时 0 毫秒
1.
Reduction of a Tetrazolium Salt in Determining Growth Activity of Yeast-Phase Histoplasma capsulatum 总被引:3,自引:0,他引:3
下载免费PDF全文
![点击此处可从《Applied microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Maria E. Reca 《Applied microbiology》1968,16(2):236-238
A colorimetric method using a tetrazolium compound, 3,4,5-dimethylthiozalil-(1 or 2),2,5-diphenyltetrazolium bromide (TTBr), was developed for studying the growth activity of yeast-phase Histoplasma capsulatum. Materials extracted in phosphate buffer, pH 7.0, from cells at different stages of growth reduced TTBr. Colorimetric changes were correlated with enzymatic activity. Under standardized conditions specified herein, the optical density of the reduced tetrazole was an index of the growth activity of the organism. 相似文献
2.
Knud IB Christensen 《Nordic Journal of Botany》1983,2(6):537-548
Crataegus curvisepala Lindm., C. laevigata (Poiret) DC. and C. monogyna Jacq. (Rosaceae) form hybrid complexes in Denmark due to introgression. C. palmstruchii Lindm. seems to be variously introgressed individuals of C. laevigata. C. eremitagensis Raunk., C. raavadensis Raunk. and C. schumacheri Raunk. apparently belong to C. curvisepala x laevigata. The delimitation between C. curvisepala x laevigata and C. laevigata x monogyna is discussed. 相似文献
3.
ZBARSKYIB 《Cell research》1998,8(2):99-103
The nonchromatin proteinous residue of the cell nucleus was revealed in our laboratory as early as in 1948 and then identified by light and electron microscopy as residual nucleoli,intranuclear network and nuclear envelope before 1960,This structure termed afterwards as “nuclear residue“,“nuclear skeleton“,“nuclear cage“,“nuclear carcass“etc.,was much later(in 1974) isolated,studied and entitled as “nuclear matrix“ by Berezney and Coffey,to whom the discovery of this residual structure is often wronly ascribed.The real history of nuclear matrix manifestation is reported in this paper. 相似文献
4.
JUAN DIEGO IBÁÑEZ‐ÁLAMO MANUEL SOLER 《Biological journal of the Linnean Society. Linnean Society of London》2010,101(4):759-766
Urbanization, one of the most extreme land‐use alterations, is currently spreading, and the number of species confronting these changes is increasing. However, contradictory results of previous studies impede a clear interpretation of which selective pressure (nest predation or food limitation) is more important in urban habitats compared with natural situations, and whether birds can confront them by adjusting their life‐history strategies. We investigated life‐history syndromes of three common blackbird (Turdus merula) populations differing in their human influence (urban, rural, and woodland). We analysed daily nest predation and nestling starvation rates to assess the relative importance of these selection pressures in each habitat. Simultaneously, several life‐history traits were investigated to determine if T. merula seem adapted to their main source of selection. Food limitation was more important in the city, whereas nest predation was the most important selective force in the forest. The rural habitat was characterized by an intermediate influence of these two factors. Life‐history syndromes, as the covariation of a suite of traits, confirmed these results because T. merula seem well adapted to the main cause of selection in each habitat. Our results are consistent with urbanization imposing new challenges on birds, and that they adaptively respond to them. © 2010 The Linnean Society of London, Biological Journal of the Linnean Society, 2010, 101 , 759–766. 相似文献
5.
Vital staining of Histoplasma capsulatum with Janus Green B 总被引:11,自引:0,他引:11
6.
Joleen S. Cheah Kyle A. Jacobs Tzu Wei Lai Reca Caballelo Jacqueline L. Yee Shuji Ueda Volkmar Heinrich Soichiro Yamada 《Molecular biology of the cell》2021,32(13):1221
Sensing physical forces is a critical first step in mechano-transduction of cells. Zyxin, a LIM domain-containing protein, is recruited to force-bearing actin filaments and is thought to repair and strengthen them. Yet, the precise force-induced protein interactions surrounding zyxin remain unclear. Using BioID analysis, we identified proximal proteins surrounding zyxin under normal and force-bearing conditions by label-free mass spectrometry analysis. Under force-bearing conditions, increased biotinylation of α-actinin 1, α-actinin 4, and AFAP1 were detected, and these proteins accumulated along force-bearing actin fibers independently from zyxin, albeit at a lower intensity than zyxin. VASP also accumulated along force-bearing actin fibers in a zyxin-dependent manner, but the biotinylation of VASP remained constant regardless of force, supporting the model of a free zyxin–VASP complex in the cytoplasm being corecruited to tensed actin fibers. In addition, ARHGAP42, a RhoA GAP, was also identified as a proximal protein of zyxin and colocalized with zyxin along contractile actin bundles. The overexpression of ARHGAP42 reduced the rate of small wound closure, a zyxin-dependent process. These results demonstrate that the application of proximal biotinylation can resolve the proximity and composition of protein complexes as a function of force, which had not been possible with traditional biochemical analysis. 相似文献
7.
Julieta Reppetti Alejandra Reca E. Abril Seyahian Yollyseth Medina Nora Martínez Natalia Szpilbarg Alicia E. Damiano 《Journal of cellular physiology》2020,235(4):3382-3392
Caveolae constitute membrane domains critical for the organization and synchronization of different signaling molecules related to numerous cell processes such as cell migration, invasion, and differentiation. Caveolin-1 (Cav-1) is the main integral membrane protein of these domains. Recently, it was found that a normal expression of aquaporin-3 (AQP3) is required for extravillous trophoblast (EVT) cell migration. Our aim was to investigate the role of caveolae in the migration, invasion, and endovascular differentiation of human EVT cells during placentation and its interaction with AQP3. EVT cells (Swan 71 cell line) were cultured in complete Dulbecco's modified Eagle's medium–nutrient mixture F12 and treated with 5 mM methyl-β-cyclodextrin (MβCD) to disrupt caveolae. We found that after MβCD treatment, Cav-1 protein was undetectable. In this condition, the ability of the cells to migrate was significantly decreased compared with the control cells, while no differences were observed in the number of invading cells and the metalloproteinases activity between control and MβCD-treated cells. Surprisingly, the disruption of caveolae significantly enhanced EVT endovascular differentiation. On the contrary, the silencing of AQP3, negatively affected tube-like formation. The theoretical analysis of the primary sequence of AQP3 protein revealed a putative Cav-1-binding site. In addition, immunoprecipitation and double immunofluorescence assays showed that AQP3 colocalized with Cav-1. These results showed that during placentation an intact caveola in EVT cells may be necessary for AQP3 and Cav-1 interaction and any perturbations might result in serious pregnancy disorders. 相似文献
8.
Protoplasts of systemic dimorphic fungal pathogens: Histoplasma capsulatum and blastomyces dermatitidis 总被引:2,自引:0,他引:2
Summary Protoplasts have been released fromH. capsulatum in the mycelial and yeast phases and from the mycelial and incompletly converted yeast phase ofB. dermatitidis by the enzymatic action of snail digestive juice. There is great variation in the mode of protoplast formation not only between species but between the two morphological forms, particularly inH. capsulatum
These studies were supported in part by grants from the American Thoracic Socicty and the United States Public Health Service National Institute of Allergy and Infectious Diseases (1-R1-AI-7520-01). 相似文献
9.
Emerging concept of cancer as a stem cell disorder 总被引:2,自引:0,他引:2
Mariusz Z. Ratajczak Magda Kucia Hanna Dobrowolska Jens Wanzeck Ryan Reca Janina Ratajczak 《Central European Journal of Biology》2006,1(1):73-87
Evidence has accumulated that malignancy arises from maturation arrest of stem cells — rather than the dedifferentiation of
somatic cells. To support this notion, stem cells in contrast to somatic cells are long lived cells and thus may become the
subject of accumulating mutations that are crucial for the initiation/progression of cancer. More importantly they may maintain
these mutations and pass them to daughter stem cells. Cancer stem cells (CSC) that derive from transformed normal stem cells
(NSC) are responsible not only for tumor initiation, but also for its re-growth and metastasis. Accumulating evidence also
indicates that adult tissues may contain a population of very small embryonic like (VSEL) stem cells that may give rise to
some very immature tumors e.g., pediatric sarcomas. Similar molecular mechanisms operating in NSC and CSC regulate resistance
to radio-chemotherapy and promote migration/metastasis. Thus, by studying the biology of NSC we can learn more about cancer. 相似文献
10.
IB Masters MM Eastburn PW Francis R Wootton PV Zimmerman RS Ware AB Chang 《Respiratory research》2005,6(1):16