全文获取类型
收费全文 | 227篇 |
免费 | 31篇 |
专业分类
258篇 |
出版年
2023年 | 2篇 |
2022年 | 7篇 |
2021年 | 9篇 |
2020年 | 6篇 |
2019年 | 3篇 |
2018年 | 5篇 |
2017年 | 5篇 |
2016年 | 8篇 |
2015年 | 9篇 |
2014年 | 12篇 |
2013年 | 16篇 |
2012年 | 11篇 |
2011年 | 9篇 |
2010年 | 16篇 |
2009年 | 9篇 |
2008年 | 8篇 |
2007年 | 9篇 |
2006年 | 2篇 |
2005年 | 2篇 |
2004年 | 4篇 |
2003年 | 3篇 |
2002年 | 2篇 |
2001年 | 4篇 |
2000年 | 2篇 |
1999年 | 4篇 |
1998年 | 4篇 |
1997年 | 6篇 |
1996年 | 6篇 |
1995年 | 3篇 |
1993年 | 3篇 |
1992年 | 3篇 |
1991年 | 5篇 |
1990年 | 4篇 |
1988年 | 4篇 |
1985年 | 2篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1977年 | 2篇 |
1975年 | 2篇 |
1974年 | 3篇 |
1970年 | 2篇 |
1968年 | 2篇 |
1959年 | 2篇 |
1958年 | 5篇 |
1956年 | 2篇 |
1955年 | 2篇 |
1954年 | 2篇 |
1953年 | 4篇 |
1952年 | 5篇 |
1951年 | 2篇 |
排序方式: 共有258条查询结果,搜索用时 0 毫秒
1.
The events that characterize egg formation and maturation inPteris cretica were investigated using transmission electronmicroscopy and electron microscope microprobe analysis. Theydid not differ significantly from those described for sexuallyreproducing ferns. The significance of these findings is discussedin relation to current theories concerning phase change in ferns. Pteris cretica, fern, apogamy, agamospory, transmission electron microscopy, oogenesis 相似文献
2.
3.
4.
Henipaviruses and lyssaviruses target nucleolar treacle protein and regulate ribosomal RNA synthesis
Stephen M. Rawlinson Tianyue Zhao Katie Ardipradja Yilin Zhang Patrick F. Veugelers Jennifer A. Harper Cassandra T. David Vinod Sundaramoorthy Gregory W. Moseley 《Traffic (Copenhagen, Denmark)》2023,24(3):146-157
The nucleolus is a common target of viruses and viral proteins, but for many viruses the functional outcomes and significance of this targeting remains unresolved. Recently, the first intranucleolar function of a protein of a cytoplasmically-replicating negative-sense RNA virus (NSV) was identified, with the finding that the matrix (M) protein of Hendra virus (HeV) (genus Henipavirus, family Paramyxoviridae) interacts with Treacle protein within nucleolar subcompartments and mimics a cellular mechanism of the nucleolar DNA-damage response (DDR) to suppress ribosomal RNA (rRNA) synthesis. Whether other viruses utilise this mechanism has not been examined. We report that sub-nucleolar Treacle targeting and modulation is conserved between M proteins of multiple Henipaviruses, including Nipah virus and other potentially zoonotic viruses. Furthermore, this function is also evident for P3 protein of rabies virus, the prototype virus of a different RNA virus family (Rhabdoviridae), with Treacle depletion in cells also found to impact virus production. These data indicate that unrelated proteins of viruses from different families have independently developed nucleolar/Treacle targeting function, but that modulation of Treacle has distinct effects on infection. Thus, subversion of Treacle may be an important process in infection by diverse NSVs, and so could provide novel targets for antiviral approaches with broad specificity. 相似文献
5.
6.
Modifications of anionic-lipid domains preceding membrane fusion in guinea pig sperm 总被引:19,自引:15,他引:4 下载免费PDF全文
The relationship between anionic-lipid concentration and the functional properties of plasma-membrane domains was explored using the guinea-pig sperm membrane as a model, with polymyxin B (PXB) as a probe. Areas of plasmalemma specialized for fusion during the acrosome reaction had a higher affinity for the probe than adjacent nonfusigenic regions. In addition, capacitation--a process preceding acrosome:plasma-membrane fusion--markedly enlarged the area susceptible to PXB binding over the acrosomal cap. Protease treatment mimicked capacitation by increasing the acrosome-reaction incidence as well as PXB binding, at enzyme concentrations not affecting the surface coat nor altering filipin/sterol localization. Both proteolytic digestion and capacitation failed to augment PXB- or filipin-affinity in nonfusigenic zones, such as the post-acrosomal segment, including its particle-free maculae. Incubation of sperm in capacitating medium supplemented with 32P-labeled phosphate, followed by lipid extraction, thin-layer chromatography, and autoradiography, revealed a radioactive band comigrating with cardiolipin and phosphatidic acid. Vermiform protrusions elicited by PXB in the outer lamellae of cardiolipin- phosphatidylcholine liposomes resembled those seen in fusional regions of sperm membrane. We conclude that (a) differing concentrations of anionic lipids are found in adjacent domains of the sperm plasma membrane; (b) these domains mirror the functional regions of the membrane, with higher anionic-lipid concentrations localized over fusional zones; (c) the surface coat does not participate in the maintenance of such domains; (d) anionic-lipid synthesis may contribute to their formation; and (e) anionic-lipid concentrations increase as the membrane becomes fusionally competent, indicating that cellular modulation of lipid domains accompanies regulation of membrane function. 相似文献
7.
8.
GUY WOODWARD JOHN B. DYBKJÆR JÓN S. ÓLAFSSON GÍSLI M. GÍSLASON ELÍSABET R. HANNESDÓTTIR NIKOLAI FRIBERG 《Global Change Biology》2010,16(7):1979-1991
The Earth is experiencing historically unprecedented rates of warming, with surface temperatures projected to increase by 3–5 °C globally, and up to 7.5 °C in high latitudes, within the next century. Knowledge of how this will affect biological systems is still largely restricted to the lower levels of organization (e.g. species range shifts), rather than at the community, food web or ecosystem level, where responses cannot be predicted from studying single species in isolation. Further, many correlational studies are confounded with time and/or space, whereas experiments have been mostly confined to laboratory microcosms that cannot capture the true complexity of natural ecosystems. We used a ‘natural experiment’ in an attempt to circumvent these shortcomings, by characterizing community structure and trophic interactions in 15 geothermal Icelandic streams ranging in temperature from 5 °C to 45 °C. Even modest temperature increases had dramatic effects across multiple levels of organization, from changes in the mean body size of the top predators, to unimodal responses of species populations, turnover in community composition, and lengthening of food chains. Our results reveal that the rates of warming predicted for the next century have serious implications for the structure and functioning of these fragile ‘sentinel’ ecosystems across multiple levels of organization. 相似文献
9.
10.