全文获取类型
收费全文 | 347篇 |
免费 | 74篇 |
专业分类
421篇 |
出版年
2016年 | 6篇 |
2015年 | 11篇 |
2014年 | 9篇 |
2013年 | 8篇 |
2012年 | 12篇 |
2011年 | 11篇 |
2010年 | 11篇 |
2009年 | 7篇 |
2008年 | 16篇 |
2007年 | 13篇 |
2006年 | 19篇 |
2005年 | 17篇 |
2004年 | 11篇 |
2003年 | 14篇 |
2002年 | 13篇 |
2001年 | 17篇 |
2000年 | 23篇 |
1999年 | 9篇 |
1998年 | 7篇 |
1997年 | 4篇 |
1996年 | 7篇 |
1995年 | 3篇 |
1993年 | 3篇 |
1992年 | 13篇 |
1991年 | 12篇 |
1990年 | 6篇 |
1989年 | 7篇 |
1988年 | 6篇 |
1987年 | 8篇 |
1986年 | 8篇 |
1985年 | 10篇 |
1984年 | 5篇 |
1983年 | 9篇 |
1981年 | 2篇 |
1980年 | 3篇 |
1979年 | 3篇 |
1978年 | 5篇 |
1977年 | 7篇 |
1976年 | 3篇 |
1975年 | 3篇 |
1974年 | 8篇 |
1973年 | 6篇 |
1972年 | 3篇 |
1971年 | 6篇 |
1970年 | 2篇 |
1969年 | 3篇 |
1968年 | 5篇 |
1966年 | 3篇 |
1948年 | 2篇 |
1931年 | 2篇 |
排序方式: 共有421条查询结果,搜索用时 15 毫秒
1.
2.
M V Kolmakova Z R Kuskova G M Ratner L M Laptakova 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1989,(5):47-50
Horse serum albumin has been shown to meet the requirements to protein preparations for microanalysis and thus to be suitable for use in kits of reagents for the radioimmunological determination of insulin and myoglobin, for the determination of tick-borne encephalitis virus antigen by the method of the enzyme immunoassay and for the stabilization of proteins in the hemagglutination test and the hemagglutination inhibition test. 相似文献
3.
Evolution of the 28S ribosomal RNA gene in anurans: regions of variability and their phylogenetic implications 总被引:1,自引:0,他引:1
Fifteen restriction sites were mapped to the 28S ribosomal RNA gene of
individuals representing 54 species of frogs, two species of salamanders, a
caecilian, and a lungfish. Eight of these sites were present in all species
examined, and two were found in all but one species. Alignment of these
conserved restriction sites revealed, among anuran 28S rRNA genes, five
regions of major length variation that correspond to four of 12 previously
identified divergent domains of this gene. One of the divergent domains
(DD8) consists of two regions of length variation separated by a short
segment that is conserved at least throughout tetrapods. Most of the
insertions, deletions, and restriction-site variations identified in the
28S gene will require sequence-level analysis for a detailed reconstruction
of their history. However, an insertion in DD9 that is coextensive with
frogs in the suborder Neobatrachia, a BstEII site that is limited to
representatives of two leptodactylid subfamilies, and a deletion in DD10
that is found only in three ranoid genera are probably synapomorphies.
相似文献
4.
Nucleotide sequence analysis of a variant human T-cell leukemia virus (HTLV-Ib) provirus with a deletion in pX-I. 总被引:11,自引:9,他引:2 下载免费PDF全文
L Ratner S F Josephs B Starcich B Hahn G M Shaw R C Gallo F Wong-Staal 《Journal of virology》1985,54(3):781-790
A variant of human T-cell leukemia virus subgroup I (HTLV-I), designated HTLV-Ib, has been isolated from a transformed T-lymphocytic cell line established from a Zairian patient with adult T-cell lymphoma. A recombinant phage clone of the variant provirus, denoted lambda MC-1, hybridizes under high stringency to HTLV-I DNA probes, but 17 of 43 restriction enzyme sites differ from those of HTLV-I, 10 of them clustering within 1.5 kilobases in the env-pX region. Since this variant virus retains its capacity to transform T-cells in vitro, and since a pX product is suspected to be important in transformation, we have determined the nucleotide sequence of the entire pX region of this virus for comparison to the prototype HTLV-I. In addition, the region between the gag and pol genes, parts of the pol and env genes, and a portion of the U3 region of the long terminal repeat sequence were also analyzed. We noted 141 single-base-pair changes among 3,897 base pairs, which were relatively well distributed over those portions of the provirus that were examined. In addition, an 11-base-pair deletion was found which included the potential initiator ATG codon of the first open reading frame of pX (pX-I). The next potential initiator codon predicted by the sequence is followed by 10 codons and then a termination codon. An identical deletion was also demonstrated in the only provirus present in another cell line established from the same patient on a different occasion after transformation in vitro of normal human umbilical cord blood cells. These results indicate that pX-I is not required for transformation. 相似文献
5.
6.
Surface modification of polymers: chemical, biological and surface analytical challenges 总被引:3,自引:0,他引:3
Buddy D. Ratner 《Biosensors & bioelectronics》1995,10(9-10):797-804
Surface modification methods can optimise the biocompatibility or the specificity of biointeraction of a biosensor or medical device. With only the surface modified, the manufacture and implantation protocol remain unchanged. This review article summarises some of the chemical, surface analytical and biological challenges associated with surface modification of biosensors and biomedical devices. 相似文献
7.
8.
Data on the subject of cell-mediated cytotoxicity suggest that no single mechanism is likely to provide a satisfactory explanation of this process. Lytic pathways have been proposed that involve both the effector cell and the target cell as active participants. In this report we describe a system in which the target cell is rendered unable to participate in its own demise. Using sheep E derivatized with CD3 antibodies, we show that metabolic inhibition of SRBC by depleting intracellular ATP with iodoacetamide, or even conversion of SRBC to "ghosts" by hypotonic lysis and resealing, has no effect on cytolysis. In the presence of EGTA or cholera toxin, both of which inhibit CTL degranulation, there is a strong suppression of both serine esterase release and cytolysis. These data show clearly that in some situations CTL are able to lyse target cells without any active participation by the target cells themselves. 相似文献
9.
10.
Conserved cysteine residues in the human immunodeficiency virus type 1 transmembrane envelope protein are essential for precursor envelope cleavage. 总被引:3,自引:3,他引:0 下载免费PDF全文
The transmembrane (TM) protein of human immunodeficiency virus type 1 has been demonstrated to be involved in viral infectivity and syncytium formation. Two highly conserved cysteine residues in the extracellular region of the TM protein are shown to be essential for processing the 160-kDa envelope precursor into the active 120- and 41-kDa mature forms. 相似文献