Lakes as nitrous oxide sources in the boreal landscape

Estimates of regional and global freshwater N₂O emissions have remained inaccurate due to scarce data and complexity of the multiple processes driving N₂O fluxes the focus predominantly being on summer time measurements from emission hot spots, agricultural streams. Here, we present four‐season data of N₂O concentrations in the water columns of randomly selected boreal lakes covering a large variation in latitude, lake type, area, depth, water chemistry, and land use cover. Nitrate was the key driver for N₂O dynamics, explaining as much as 78% of the variation of the seasonal mean N₂O concentrations across all lakes. Nitrate concentrations varied among seasons being highest in winter and lowest in summer. Of the surface water samples, 71% were oversaturated with N₂O relative to the atmosphere. Largest oversaturation was measured in winter and lowest in summer stressing the importance to include full year N₂O measurements in annual emission estimates. Including winter data resulted in fourfold annual N₂O emission estimates compared to summer only measurements. Nutrient‐rich calcareous and large humic lakes had the highest annual N₂O emissions. Our emission estimates for Finnish and boreal lakes are 0.6 and 29 Gg N₂O‐N/year, respectively. The global warming potential of N₂O from lakes cannot be neglected in the boreal landscape, being 35% of that of diffusive CH₄ emission in Finnish lakes.     

Controls of organic and inorganic carbon in randomly selected Boreal lakes in varied catchments

Organic and inorganic carbon concentrations in lakes and the links to catchment and water quality were studied in variable landscapes using the Finnish Lake Survey data base including 874 randomly selected lakes sampled during autumn overturn. The median total organic carbon (TOC) in these boreal lakes was 7.8 mg l^?1, the median total inorganic carbon (TIC) 1.6 mg l^?1 and the median partial pressure of CO₂ (pCO₂) 900 μatm. When the data was divided into subgroups according to land use in the catchment, the proportion of TIC of the total carbon (TC) in lakes was highest (31%) in agricultural areas and lowest (10%) in peatland areas. Elevated TIC concentrations were associated with agricultural land in the catchment, whereas elevated TOC concentrations were observed in lakes with high peatland proportion in the catchment. Two contrasting important sources of CO₂ in lakes were identified on the basis of statistical analysis of the data; weathering processes in the catchments and decomposition of organic matter. CO₂ was also strongly associated with total nutrients TN and TP, implying the importance of quality of organic matter and availability of nutrients for the decomposition processes.     

Characterization of adhesive epitopes with the OmpS display system.

OmpS is an outer membrane protein of Vibrio cholerae where it forms trimeric pores that function in the uptake of maltose and maltodextrins. Based on sequence similarity to LamB proteins, a model of OmpS folding in the outer membrane has been constructed. According to this model, OmpS contains 18 transmembrane beta-strands and nine surface-accessible loops. Adhesive epitopes can, when inserted into surface-accessible loop 4 (L4) and expressed in Escherichia coli, retain their functional characteristics. We inserted three D-repeats from the Staphylococcus aureus fibronectin-binding protein FnBPA into L4 of OmpS and showed that E. coli cells expressing these hybrids bind fibronectin. DNA fragments covering the N-terminal half of the globoside-binding P-fimbrial adhesin class II PapG of E. coli were cloned into the same surface accessible loop (L4) of OmpS. Fragments of papG encoding 53 or 186 amino acids from the N-terminal end of class II PapG adhesin were found to confer bacterial adhesiveness to globoside. Removal of 23 amino acids from the N-terminus of PapG did not affect receptor binding, but removal of 31 amino acids abolished it. The newly developed night sky image technique was also used to demonstrate the binding properties of membrane vesicles carrying the hybrid proteins. We raised antibodies against the purified hybrid protein containing 53 amino acids from PapG. This antiserum recognized the P-fimbriae on E. coli cells. These data provide evidence that the N-terminal first 53 amino acids of class II PapG contain the receptor-binding domain.     

Placenta defects and embryonic lethality resulting from disruption of mouse hydroxysteroid (17-beta) dehydrogenase 2 gene

Hydroxysteroid (17-beta) dehydrogenase 2 (HSD17B2) is a member of aldo-keto reductase superfamily, known to catalyze the inactivation of 17beta-hydroxysteroids to less active 17-keto forms and catalyze the conversion of 20alpha-hydroxyprogesterone to progesterone in vitro. To examine the role of HSD17B2 in vivo, we generated mice deficient in Hsd17b2 [HSD17B2 knockout (KO)] by a targeted gene disruption in embryonic stem cells. From the homozygous mice carrying the disrupted Hsd17b2, 70% showed embryonic lethality appearing at the age of embryonic d 11.5 onward. The embryonic lethality was associated with reduced placental size measured at embryonic d 17.5. The HSD17B2KO mice placentas presented with structural abnormalities in all three major layers: the decidua, spongiotrophoblast, and labyrinth. Most notable was the disruption of the spongiotrophoblast and labyrinthine layers, together with liquid-filled cysts in the junctional region and the basal layer. Treatments with an antiestrogen or progesterone did not rescue the embryonic lethality or the placenta defect in the homozygous mice. In hybrid background used, 24% of HSD17B2KO mice survived through the fetal period but were born growth retarded and displayed a phenotype in the brain with enlargement of ventricles, abnormal laminar organization, and increased cellular density in the cortex. Furthermore, the HSD17B2KO mice had unilateral renal degeneration, the affected kidney frequently appearing as a fluid-filled sac. Our results provide evidence for a role for HSD17B2 enzyme in the cellular organization of the mouse placenta.     

Regional Variability and Drivers of Below Ice CO<Subscript>2</Subscript> in Boreal and Subarctic Lakes

Northern lakes are ice-covered for considerable portions of the year, where carbon dioxide (CO₂) can accumulate below ice, subsequently leading to high CO₂ emissions at ice-melt. Current knowledge on the regional control and variability of below ice partial pressure of carbon dioxide (pCO₂) is lacking, creating a gap in our understanding of how ice cover dynamics affect the CO₂ accumulation below ice and therefore CO₂ emissions from inland waters during the ice-melt period. To narrow this gap, we identified the drivers of below ice pCO₂ variation across 506 Swedish and Finnish lakes using water chemistry, lake morphometry, catchment characteristics, lake position, and climate variables. We found that lake depth and trophic status were the most important variables explaining variations in below ice pCO₂ across the 506 lakes_. Together, lake morphometry and water chemistry explained 53% of the site-to-site variation in below ice pCO₂. Regional climate (including ice cover duration) and latitude only explained 7% of the variation in below ice pCO₂. Thus, our results suggest that on a regional scale a shortening of the ice cover period on lakes may not directly affect the accumulation of CO₂ below ice but rather indirectly through increased mobility of nutrients and carbon loading to lakes. Thus, given that climate-induced changes are most evident in northern ecosystems, adequately predicting the consequences of a changing climate on future CO₂ emission estimates from northern lakes involves monitoring changes not only to ice cover but also to changes in the trophic status of lakes.     

Type III secretion contributes to the pathogenesis of the soft-rot pathogen Erwinia carotovora: partial characterization of the hrp gene cluster

The virulence of soft-rot Erwinia species is dependent mainly upon secreted enzymes such as pectinases, pectin lyases, and proteases that cause maceration of plant tissue. Some soft-rot Erwinia spp. also harbor genes homologous to the hypersensitive reaction and pathogenesis (hrp) gene cluster, encoding components of the type III secretion system. The hrp genes are essential virulence determinants for numerous nonmacerating gram-negative plant pathogens but their role in the virulence of soft-rot Erwinia spp. is not clear. We isolated and characterized 11 hrp genes of Erwinia carotovora subsp. carotovora. Three putative sigmaL-dependent Hrp box promoter sequences were found. The genes were expressed when the bacteria were grown in Hrp-inducing medium. The operon structure of the hrp genes was determined by mRNA hybridization, and the results were in accordance with the location of the Hrp boxes. An E. carotovora strain with mutated hrcC, an essential hrp gene, was constructed. The hrcC- strain was able to multiply and cause disease in Arabidopsis, but the population kinetics were altered so that growth was delayed during the early stages of infection.     

SHARPIN regulates collagen architecture and ductal outgrowth in the developing mouse mammary gland

SHARPIN is a widely expressed multifunctional protein implicated in cancer, inflammation, linear ubiquitination and integrin activity inhibition; however, its contribution to epithelial homeostasis remains poorly understood. Here, we examined the role of SHARPIN in mammary gland development, a process strongly regulated by epithelial–stromal interactions. Mice lacking SHARPIN expression in all cells (Sharpin^cpdm), and mice with a stromal (S100a4‐Cre) deletion of Sharpin, have reduced mammary ductal outgrowth during puberty. In contrast, Sharpin^cpdm mammary epithelial cells transplanted in vivo into wild‐type stroma, fully repopulate the mammary gland fat pad, undergo unperturbed ductal outgrowth and terminal differentiation. Thus, SHARPIN is required in mammary gland stroma during development. Accordingly, stroma adjacent to invading mammary ducts of Sharpin^cpdm mice displayed reduced collagen arrangement and extracellular matrix (ECM) stiffness. Moreover, Sharpin^cpdm mammary gland stromal fibroblasts demonstrated defects in collagen fibre assembly, collagen contraction and degradation in vitro. Together, these data imply that SHARPIN regulates the normal invasive mammary gland branching morphogenesis in an epithelial cell extrinsic manner by controlling the organisation of the stromal ECM.     

A large carbon pool and small sink in boreal Holocene lake sediments

Model‐based estimates suggest that lake sediments may be a significant, long‐term sink for organic carbon (C) at regional to global scales. These models have used various approaches to predict sediment storage at broad scales from very limited data sets. Here, we report a large‐scale direct assessment of the standing stock and sedimentation rate of C for a representative set of lakes in Finland. The 122 lakes were selected from the statistically selected Nordic Lake Survey database, they cover the entire country and the water quality represents the average lake water quality in Finland. Unlike all prior estimates, these data use sediment cores that comprise the entire sediment record. The data show that within Finland, aquatic ecosystems contain the second largest areal C stocks (19 kg C m^?2) after peatlands (72 kg C m^?2), and exceed by significant amounts stocks in the forest soil (uppermost 75cm; 7.2 kg C m^?2) and woody biomass (3.4 kg C m^?2). Kauppi et al. (1997). The Finnish estimate extrapolated over the boreal region gives a total C pool in lakes 19–27 Pg C, significantly lower than the previous model‐based estimates.     

Midsummer spatial variation in methane efflux from stands of littoral vegetation in a boreal meso-eutrophic lake

1. Spatial variation of methane (CH₄) efflux from the littoral zone of a meso‐eutrophic boreal lake was studied with a closed‐chamber technique for three summer days in 22 vegetation stands, consisting of three emergent and three floating‐leaved species. 2. Between‐species differences in CH₄ emission were significant. The highest emissions were measured from the emergent Phragmites australis stands (0.5–1.7 mmol m^?2 h^?1), followed by Schoenoplectus lacustris > Equisetum fluviatile > Nuphar lutea > Sparganium gramineum > Potamogeton natans. Within‐species differences between stands were not significant. 3. In P. australis stands, the stand‐specific mean CH₄ emission was significantly correlated with solar radiation, probably indicating the role of effective pressurised ventilation on CH₄ fluxes. The proportion of net primary production emitted as CH₄ was significantly higher in P. australis stands (7.4%) than in stands of S. lacustris and E. fluviatile (both 0.5%). 4. In N. lutea stands, CH₄ efflux was negatively correlated with the mean fetch and positively with the percentage cover of leaves on the water surface. There were no differences in CH₄ efflux between intact N. lutea leaves and those grazed by coleopteran Galerucella nymphaeae. In S. graminaeum and P. natans stands, CH₄ effluxes were not related to any of the measured environmental variables. 5. For all vegetation stands, the biomass above water level explained about 60% of the observed spatial variation in CH₄ emission, indicating the important role of plants as gas conduits and producers of substrates for methanogens in the anoxic sediment.     

Carbon dynamics in a Boreal land-stream-lake continuum during the spring freshet of two hydrologically contrasting years

We studied in 2013 and 2014 the spring carbon dynamics in a Boreal landscape consisting of a lake and 15 inflowing streams and an outlet. The first year had weather and a hydrological regime typical of past years with a distinct spring freshet connected with the thaw of the average snowpack. The latter year had higher air temperatures which did not permit snow accumulation, despite similar winter precipitation. As such, there was hardly any spring freshet in 2014, and stream discharge peaked in January, i.e., the conditions resembled those predicted in the future climate. Despite the hydrological differences between the years, there were only small interannual differences in the stream CO₂ and DOC concentrations. The relationship between the concentrations and discharge was stronger in the typical year. CO₂ concentrations in medium-sized streams correlated negatively with the discharge, indicating dilution effect of melting snowpacks, while in large-sized streams the correlation was positive, suggesting stronger groundwater influence. The DOC pathway to these streams was through the subsurface soil layers, not the groundwater. The total amount of carbon transported into the lake was ca. 1.5-fold higher in the typical year than in the year with warm winter. In 2013, most of the lateral inputs took place during spring freshet. In 2014, the majority of inputs occurred earlier, during the winter months. The lateral CO₂ signal was visible in the lake at 1.5 m depth. DOC dominated the carbon transport, and in both years, 12% of the input C was in inorganic form.