全文获取类型
收费全文 | 2022篇 |
免费 | 222篇 |
出版年
2021年 | 21篇 |
2020年 | 19篇 |
2019年 | 25篇 |
2018年 | 22篇 |
2017年 | 26篇 |
2016年 | 35篇 |
2015年 | 66篇 |
2014年 | 62篇 |
2013年 | 74篇 |
2012年 | 97篇 |
2011年 | 80篇 |
2010年 | 55篇 |
2009年 | 49篇 |
2008年 | 74篇 |
2007年 | 72篇 |
2006年 | 58篇 |
2005年 | 76篇 |
2004年 | 60篇 |
2003年 | 55篇 |
2002年 | 57篇 |
2001年 | 73篇 |
2000年 | 64篇 |
1999年 | 57篇 |
1998年 | 27篇 |
1997年 | 32篇 |
1996年 | 40篇 |
1995年 | 17篇 |
1994年 | 23篇 |
1993年 | 17篇 |
1992年 | 38篇 |
1991年 | 32篇 |
1990年 | 35篇 |
1989年 | 51篇 |
1988年 | 33篇 |
1987年 | 36篇 |
1986年 | 28篇 |
1985年 | 23篇 |
1984年 | 29篇 |
1983年 | 28篇 |
1982年 | 13篇 |
1981年 | 13篇 |
1979年 | 16篇 |
1975年 | 15篇 |
1973年 | 13篇 |
1972年 | 13篇 |
1971年 | 13篇 |
1970年 | 19篇 |
1968年 | 14篇 |
1957年 | 13篇 |
1952年 | 12篇 |
排序方式: 共有2244条查询结果,搜索用时 15 毫秒
1.
2.
There is an urgent need for effective prophylactic measures against Mycobacterium tuberculosis (Mtb) infection, particularly given the highly variable efficacy of Bacille Calmette-Guerin (BCG), the only licensed vaccine against tuberculosis (TB). Most studies indicate that cell-mediated immune responses involving both CD4+ and CD8+ T cells are necessary for effective immunity against Mtb. Genetic vaccination induces humoral and cellular immune responses, including CD4+ and CD8+ T-cell responses, against a variety of bacterial, viral, parasitic and tumor antigens, and this strategy may therefore hold promise for the development of more effective TB vaccines. Novel formulations and delivery strategies to improve the immunogenicity of DNA-based vaccines have recently been evaluated, and have shown varying degrees of success. In the present study, we evaluated DNA-launched Venezuelan equine encephalitis replicons (Vrep) encoding a novel fusion of the mycobacterial antigens α-crystallin (Acr) and antigen 85B (Ag85B), termed Vrep-Acr/Ag85B, for their immunogenicity and protective efficacy in a murine model of pulmonary TB. Vrep-Acr/Ag85B generated antigen-specific CD4+ and CD8+ T cell responses that persisted for at least 10 wk post-immunization. Interestingly, parenterally administered Vrep-Acr/Ag85B also induced T cell responses in the lung tissues, the primary site of infection, and inhibited bacterial growth in both the lungs and spleens following aerosol challenge with Mtb. DNA-launched Vrep may, therefore, represent an effective approach to the development of gene-based vaccines against TB, particularly as components of heterologous prime-boost strategies or as BCG boosters. 相似文献
3.
4.
5.
6.
J. D. Macdonald. 《Ibis》1952,94(1):122-127
7.
K. D. Macdonald 《Applied microbiology》1972,23(5):990-993
Conidiated slope cultures of derivative of Penicillium chrysogenum Wis 54-1255 were stored at -196 or +4 C for a period of 3.5 years. After this time, the viability fell to 68% in the former case and to 4% in the latter. At the end of the experiment, 65 single conidial isolates from each series were tested for penicillin yield. Among those from conidia stored at -196 C, the spread of penicillin yields did not differ markedly from that of 65 single conidial isolates made as controls prior to storage. However, 18% of those from conidia stored at +4 C formed a subpopulation with substantially lower penicillin titers than those of control isolates. Storage at -196 C may reduce or prevent a possible source of penicillin yield decay, namely, the selection of spontaneous mutants of low titer present in small numbers in the original culture and selected, as viability decreased, by virtue of their increased longevity relative to that of the parental culture. 相似文献
8.
9.
Ion-Exchange Chromatography Separates Activities Synthesizing and Degrading Fructose 2,6-Bisphosphate from C3 and C4 Leaves but Not from Rat Liver 总被引:1,自引:1,他引:0
下载免费PDF全文
![点击此处可从《Plant physiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Fructose-6-phosphate,2-kinase and fructose-2,6-bisphosphatase were separated on the basis of charge from leaves of C3 (spinach, lettuce, and pea) and C4 (sorghum and amaranthus) plants but not from rat liver—a tissue known to contain a bifunctional enzyme with both activities. [2-32P]Fructose 2,6-bisphosphate binding experiments also suggest that the major forms of these activities reside on different proteins in leaves. 相似文献
10.
Stopped-flow studies on the mechanism of oxidation of N-methyl-4-phenyltetrahydropyridine by bovine liver monoamine oxidase B 总被引:2,自引:0,他引:2
The kinetic mechanism of monoamine oxidase B involves either a binary or a ternary complex, depending on the substrate. In this study, stopped-flow kinetic data provide direct evidence for ternary complexes not only of reduced enzyme, oxygen, and product but also of reduced enzyme, oxygen, and substrate, both for benzylamine and for the tertiary amine 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). However, the mechanism for a given substrate is not exclusive but, rather, is determined by competition between the alternate pathways as a result of different rate constants for the oxidation of the reduced enzyme, the reduced enzyme-product complex, and the reduced enzyme-substrate complex, as well as the different dissociation constants for the complexes. Comparison of the rate constants obtained from the stopped-flow studies with steady-state data indicates that the overall rate of reaction for the oxidation of MPTP by monoamine oxidase is dominated by the reductive step, but for benzylamine the steady-state rate is determined by a complex function of the rates of both the reductive and oxidative half-reactions. 相似文献