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1.
DNA sequences were determined for three to five alleles of the bride-of-
sevenless (boss) gene in each of four species of Drosophila. The product of
boss is a transmembrane receptor for a ligand coded by the sevenless gene
that triggers differentiation of the R7 photoreceptor cell in the compound
eye. Population parameters affecting the rate and pattern of molecular
evolution of boss were estimated from the multinomial configurations of
nucleotide polymorphisms of synonymous codons. The time of divergence
between D. melanogaster and D. simulans was estimated as approximately 1
Myr, that between D. teissieri and D. yakuba as approximately 0.75 Myr, and
that between the two pairs of sibling species as approximately 2 Myr. (The
boss genes themselves have estimated divergence times approximately 50%
greater than the species divergence times.) The effective size of the
species was estimated as approximately 5 x 10(6), and the average mutation
rate was estimated as 1-2 x 10(-9)/nucleotide/generation. The ratio of
amino acid polymorphisms within species to fixed differences between
species suggests that approximately 25% of all possible single-step amino
acid replacements in the boss gene product may be selectively neutral or
nearly neutral. The data also imply that random genetic drift has been
responsible for virtually all of the observed differences in the portion of
the boss gene analyzed among the four species.
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2.
The contractile basis of amoeboid movement: V. The control of gelation, solation, and contraction in extracts from dictyostelium discoideum 总被引:29,自引:22,他引:7 下载免费PDF全文
Motile extracts have been prepared from Dictyostelium discoideum by homogenization and differential centrifugation at 4 degrees C in a stabilization solution (60). These extracts gelled on warming to 25 degrees Celsius and contracted in response to micromolar Ca++ or a pH in excess of 7.0. Optimal gelation occurred in a solution containing 2.5 mM ethylene glycol-bis (β-aminoethyl ether)N,N,N',N'-tetraacetate (EGTA), 2.5 mM piperazine-N-N'-bis [2-ethane sulfonic acid] (PIPES), 1 mM MgC1(2), 1 mM ATP, and 20 mM KCI at ph 7.0 (relaxation solution), while micromolar levels of Ca++ inhibited gelation. Conditions that solated the gel elicited contraction of extracts containing myosin. This was true regardless of whether chemical (micromolar Ca++, pH >7.0, cytochalasin B, elevated concentrations of KCI, MgC1(2), and sucrose) or physical (pressure, mechanical stress, and cold) means were used to induce solation. Myosin was definitely required for contraction. During Ca++-or pH-elicited contraction: (a) actin, myosin, and a 95,000-dalton polypeptide were concentrated in the contracted extract; (b) the gelation activity was recovered in the material sqeezed out the contracting extract;(c) electron microscopy demonstrated that the number of free, recognizable F-actin filaments increased; (d) the actomyosin MgATPase activity was stimulated by 4- to 10-fold. In the absense of myosin the Dictyostelium extract did not contract, while gelation proceeded normally. During solation of the gel in the absense of myosin: (a) electron microscopy demonstrated that the number of free, recognizable F- actin filaments increased; (b) solation-dependent contraction of the extract and the Ca++-stimulated MgATPase activity were reconstituted by adding puried Dictyostelium myosin. Actin purified from the Dictyostelium extract did not gel (at 2 mg/ml), while low concentrations of actin (0.7-2 mg/ml) that contained several contaminating components underwent rapid Ca++ regulated gelation. These results indicated : (a) gelation in Dictyostelium extracts involves a specific Ca++-sensitive interaction between actin and several other components; (b) myosin is an absolute requirement for contraction of the extract; (c) actin-myosin interactions capable of producing force for movement are prevented in the gel, while solation of the gel by either physical or chemical means results in the release of F-actin capable of interaction with myosin and subsequent contraction. The effectiveness of physical agents in producting contraction suggests that the regulation of contraction by the gel is structural in nature. 相似文献
3.
4.
V KW Wong T Li B YK Law E DL Ma N C Yip F Michelangeli C KM Law M M Zhang K YC Lam P L Chan L Liu 《Cell death & disease》2013,4(7):e720
Autophagy is an important cellular process that controls cells in a normal homeostatic state by recycling nutrients to maintain cellular energy levels for cell survival via the turnover of proteins and damaged organelles. However, persistent activation of autophagy can lead to excessive depletion of cellular organelles and essential proteins, leading to caspase-independent autophagic cell death. As such, inducing cell death through this autophagic mechanism could be an alternative approach to the treatment of cancers. Recently, we have identified a novel autophagic inducer, saikosaponin-d (Ssd), from a medicinal plant that induces autophagy in various types of cancer cells through the formation of autophagosomes as measured by GFP-LC3 puncta formation. By computational virtual docking analysis, biochemical assays and advanced live-cell imaging techniques, Ssd was shown to increase cytosolic calcium level via direct inhibition of sarcoplasmic/endoplasmic reticulum Ca2+ ATPase pump, leading to autophagy induction through the activation of the Ca2+/calmodulin-dependent kinase kinase–AMP-activated protein kinase–mammalian target of rapamycin pathway. In addition, Ssd treatment causes the disruption of calcium homeostasis, which induces endoplasmic reticulum stress as well as the unfolded protein responses pathway. Ssd also proved to be a potent cytotoxic agent in apoptosis-defective or apoptosis-resistant mouse embryonic fibroblast cells, which either lack caspases 3, 7 or 8 or had the Bax-Bak double knockout. These results provide a detailed understanding of the mechanism of action of Ssd, as a novel autophagic inducer, which has the potential of being developed into an anti-cancer agent for targeting apoptosis-resistant cancer cells. 相似文献
5.
Iris A. Holmes Ivan V. Monagan Daniel L. Rabosky Alison R. Davis Rabosky 《Ecology and evolution》2019,9(22):12471-12481
Gut microbiomes perform essential services for their hosts, including helping them to digest food and manage pathogens and parasites. Performing these services requires a diverse and constantly changing set of metabolic functions from the bacteria in the microbiome. The metabolic repertoire of the microbiome is ultimately dependent on the outcomes of the ecological interactions of its member microbes, as these interactions in part determine the taxonomic composition of the microbiome. The ecological processes that underpin the microbiome's ability to handle a variety of metabolic challenges might involve rapid turnover of the gut microbiome in response to new metabolic challenges, or it might entail maintaining sufficient diversity in the microbiome that any new metabolic demands can be met from an existing set of bacteria. To differentiate between these scenarios, we examine the gut bacteria and resident eukaryotes of two generalist‐insectivore lizards, while simultaneously identifying the arthropod prey each lizard was digesting at the time of sampling. We find that the cohorts of bacteria that occur significantly more or less often than expected with arthropod diet items or eukaryotes include bacterial species that are highly similar to each other metabolically. This pattern in the bacterial microbiome could represent an early step in the taxonomic shifts in bacterial microbiome that occur when host lineages change their diet niche over evolutionary timescales. 相似文献
6.
High rate of DNA loss in the Drosophila melanogaster and Drosophila virilis species groups 总被引:6,自引:3,他引:3
We recently proposed that patterns of evolution of non-LTR
retrotransposable elements can be used to study patterns of spontaneous
mutation. Transposition of non-LTR retrotransposable elements commonly
results in creation of 5' truncated, "dead-on-arrival" copies. These
inactive copies are effectively pseudogenes and, according to the neutral
theory, their molecular evolution ought to reflect rates and patterns of
spontaneous mutation. Maximum parsimony can be used to separate the
evolution of active lineages of a non-LTR element from the fate of the
"dead-on-arrival" insertions and to directly assess the relative
frequencies of different types of spontaneous mutations. We applied this
approach using a non-LTR element, Helena, in the Drosophila virilis group
and have demonstrated a surprisingly high incidence of large deletions and
the virtual absence of insertions. Based on these results, we suggested
that Drosophila in general may exhibit a high rate of spontaneous large
deletions and have hypothesized that such a high rate of DNA loss may help
to explain the puzzling dearth of bona fide pseudogenes in Drosophila. We
also speculated that variation in the rate of spontaneous deletion may
contribute to the divergence of genome size in different taxa by affecting
the amount of superfluous "junk" DNA such as, for example, pseudogenes or
long introns. In this paper, we extend our analysis to the D. melanogaster
subgroup, which last shared a common ancestor with the D. virilis group
approximately 40 MYA. In a different region of the same transposable
element, Helena, we demonstrate that inactive copies accumulate deletions
in species of the D. melanogaster subgroup at a rate very similar to that
of the D. virilis group. These results strongly suggest that the high rate
of DNA loss is a general feature of Drosophila and not a peculiar property
of a particular stretch of DNA in a particular species group.
相似文献
7.
Rabosky DL 《Systematic biology》2010,59(6):634-645
The disparity in species richness across the tree of life is one of the most striking and pervasive features of biological diversity. Some groups are exceptionally diverse, whereas many other groups are species poor. Differences in diversity among groups are frequently assumed to result from primary control by differential rates of net diversification. However, a major alternative explanation is that ecological and other factors exert primary control on clade diversity, such that apparent variation in net diversification rates is a secondary consequence of ecological limits on clade growth. Here, I consider a likelihood framework for distinguishing between these competing hypotheses. I incorporate hierarchical modeling to explicitly relax assumptions about the constancy of diversification rates across clades, and I propose several statistics for a posteriori evaluation of model adequacy. I apply the framework to a recent dated phylogeny of ants. My results reject the hypothesis that net diversification rates exert primary control on species richness in this group and demonstrate that clade diversity is better explained by total time-integrated speciation. These results further suggest that it may not possible to estimate meaningful speciation and extinction rates from higher-level phylogenies of extant taxa only. 相似文献
8.
9.
No substitute for real data: A cautionary note on the use of phylogenies from birth–death polytomy resolvers for downstream comparative analyses 下载免费PDF全文
Daniel L. Rabosky 《Evolution; international journal of organic evolution》2015,69(12):3207-3216
The statistical estimation of phylogenies is always associated with uncertainty, and accommodating this uncertainty is an important component of modern phylogenetic comparative analysis. The birth–death polytomy resolver is a method of accounting for phylogenetic uncertainty that places missing (unsampled) taxa onto phylogenetic trees, using taxonomic information alone. Recent studies of birds and mammals have used this approach to generate pseudoposterior distributions of phylogenetic trees that are complete at the species level, even in the absence of genetic data for many species. Many researchers have used these distributions of phylogenies for downstream evolutionary analyses that involve inferences on phenotypic evolution, geography, and community assembly. I demonstrate that the use of phylogenies constructed in this fashion is inappropriate for many questions involving traits. Because species are placed on trees at random with respect to trait values, the birth–death polytomy resolver breaks down natural patterns of trait phylogenetic structure. Inferences based on these trees are predictably and often drastically biased in a direction that depends on the underlying (true) pattern of phylogenetic structure in traits. I illustrate the severity of the phenomenon for both continuous and discrete traits using examples from a global bird phylogeny. 相似文献
10.
Daniel L. Rabosky 《PLoS biology》2021,19(8)
An influential hypothesis proposes that the tempo of evolution is faster in the tropics. Emerging evidence, including a study in this issue of PLOS Biology, challenges this view, raising new questions about the causes of Earth’s iconic latitudinal diversity gradient (LDG).Biologists have long puzzled over the spectacular diversity of animals and plants from Earth’s tropical regions. It is true that some tropical environments are not especially rich in species, and some groups of organisms show contrarian patterns with diversity peaks that occur far outside of the warm, humid tropics. Nonetheless, the big picture is clear: A vastly disproportionate fraction of Earth’s terrestrial biodiversity is concentrated in tropical rainforests, and warm water reef environments similarly account for a large fraction of marine diversity. The extremes of tropical diversity transcend the ability of most humans to process it: Some Amazonian rainforests, for example, contain more species of trees in just a few hectares than are found in the entirety of Europe or North America [1]. In general, the most diverse tropical rainforests support order-of-magnitude increases in species richness relative to otherwise comparable temperate zone communities across a wide range of organisms. Despite decades of study, however, the causes of this latitudinal diversity gradient (LDG) remain elusive.One of the most prominent hypotheses for the LDG is, loosely speaking, the idea that biological processes speed up in the tropics, potentially due to the kinetic effects of temperature on the rates of organismal processes. It seems obvious that the pulse of life should be faster under a torrid tropical sun, and—to naturalists who’ve spent time in lowland rainforests in particular—such a view accords well with perceptions of the humid tropics as a raging, steamy mess of species interactions that collectively generate the tangled web that is tropical diversity. It is generally accepted that temperature can affect metabolic rate and many other biological processes, including those involving ecological interactions between species (e.g., competition, predation, and herbivory). The specific mechanisms connecting thermal energy to biodiversity remain unclear. For example, they might involve the influence of temperature on rates of molecular evolution, which might then influence rates of speciation [2]. Or, species in warmer environments might live closer to their optimal body temperatures, thus enabling them to allocate more resources to performance-associated functions and leading to a systematic upgrading in the intensity of antagonistic or coevolutionary interactions between species [3]. Regardless of the specific mechanism, the general idea is captured by Brown [4], who notes that “‘Diversity begets diversity’ in the tropics, because ‘the Red Queen runs faster when she is hot.’”Writing in PLOS Biology, Drury and colleagues [5] demonstrate that a central prediction of these “faster tropics” hypotheses fails to hold up. They predicted that, if certain types of ecological interactions between species are stronger in the tropics, then we should see a signal of those interactions in long-term patterns of trait evolution. In particular, the increased pressure to respond to species interactions in the tropics should result in faster overall rates of morphological evolution for tropical species. To test this hypothesis, the authors studied the rate of morphological evolution in birds, analyzing a large dataset on bill shape and body proportions from other recent studies [6] with a battery of sophisticated statistical models. These models allowed the rate of morphological change to differ systematically with latitude. Intriguingly, the models that best fit the data in some cases were those that allowed for strong interactions between species in driving patterns of divergence among closely related species that occupied that same biogeographic region (e.g., the neotropics). Thus, there is a partial signal of species interactions on the morphologies of species we see living together today, including those from both tropical and temperate regions. As suggested by the authors, these patterns might reflect a form of ecological character displacement, whereby morphologically similar species evolve differences that minimize their ecological overlap. But, surprisingly, the intensity of these effects shows no consistent relationship with latitude. The take-home message is that—at least for birds and the traits considered—species are not evolving more rapidly in the tropics.Drury and colleagues note that their results contradict recent articles that have documented differences in phenotypic evolutionary rates across latitude, although the studies referenced generally looked at different types of traits (e.g., birdsong). They suggest several potential reasons for the discrepancies between their results and those prior studies. But, critically, these earlier studies generally did not report faster evolution in the tropics, but faster evolution in the temperate zone. Hence, the results of Drury and colleagues and the earlier studies all converge to a similar and more general finding, which is that the warm tropics really aren’t so hot for macroevolution, at least as far as phenotypic evolutionary rates are concerned. By rejecting the simple explanations (faster evolution), new questions emerge about how and why tropical bird communities show such dramatic phenotypic and ecological diversity.Morphological evolution is not the only process that fails to show the expected pattern of “heating up” in the tropics. A number of recent studies have found that rates of species formation are either unrelated to latitude or slower in the tropics [7–9]. These results argue strongly against temperature kinetic models of biodiversity, whereby faster speciation emerges from the effects of warmer temperatures in the tropics on mutation and metabolic rates [10]. Many of the same causal pathways that predict increased rates of speciation as a function of temperature would also apply to rates of morphological evolution: Increased mutation rates in the tropics, for example, should accelerate the tempo of phenotypic evolution due to increased mutational variance in traits. But, regardless of whether we consider phenotypic evolution (as in Drury and colleagues) or lineage diversification, there is simply no evidence for faster evolutionary rates in the tropics.The results from Drury and colleagues [5] and other studies do not reject all possible causal pathways by which temperature or species interactions might facilitate high tropical diversity. Many phylogeny-based studies of species diversification and phenotypic evolution frame their interpretations through the lens of interspecific competition, ecological opportunity, and character displacement. Yet, numerous other types of interactions are relevant to global biodiversity patterns, and some of these interactions have scarcely been explored from a macroevolutionary perspective. Many such interactions have the potential to influence species richness and ecological diversity, perhaps through mechanisms that involve an indirect effect of temperature on equilibrium diversity levels. With more data on how host–pathogen, predator–prey, and other biotic interactions vary latitudinally, perhaps we will emerge with a greater understanding of the diverse mechanisms that contribute to the spectacular enrichment of tropical diversity. 相似文献