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Laboratory experiments were conducted to measure the feeding rates, preferences, survival rates, maturation times, and weight gain of four common species of grasshoppers (Orthoptera: Acrididae) on four lines of safflower (Carthamus tinctorius). When safflower lines were presented individually, all were readily consumed although consumption differed significantly according to grasshopper species, age and sex, and to safflower line. Consumption was greatest on a safflower line devoid of spines. However, grasshoppers also fed readily on spiny lines, and results of experiments in which all safflower lines were presented together indicated preferences that did not necessarily correspond to the results of the single-line feeding trials. Camnula pellucida, a graminivorous species, did feed on safflower, but restricted feeding almost exclusively to stem cambium, whereas Melanoplus bivittatus, M. packardii and M. sanguinipes fed mainly on the heads, flowers and leaves. Highly significant differences in grasshopper maturation time and weight gain were apparent among grasshopper species, and among safflower lines. Seedtec-5, the line which was least digestible, least consumed and least preferred nevertheless yielded rapid development and the greatest body weights of grasshoppers. All species of Melanoplus developed more quickly and gained more weight on safflower than on wheat. One species, M. packardii, was more able to utilise safflower than its congeners, and may gain competitive advantage if safflower becomes widely grown. 相似文献
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WALTER C. QUEVEDO JACOB DYCKMAN RUTH HALABAN GISELA E. MOELLMANN JANET M. COWAN THOMAS J. HOLSTEIN 《Pigment cell & melanoma research》1988,1(Z1):124-131
The BULT melanoma originated at Brown University as a spontaneous, small black nodule on the tail of an adult female mouse of the LT/Ch strain. Histological examination of a portion of the tumor indicated that it was intradermal and consisted predominantly of heavily melanized, ovoid to fusiform cells with melanin-laden macrophages scattered among them. The BULT melanoma has been maintained in LT/Ch mice for approximately 5 years by periodic transplantation, at first subcutaneously on the flanks and, more recently, intramuscularly in the hind legs. The shift in transplantation site was made following a marked decline in the growth of subcutaneous grafts. The transplants have retained the uniform deep-black melanization and general histology of the primary melanoma. Numerous melanosomes at all stages of development are found within the melanoma cells. DOPA-positive cytoplasmic vesicles are abundant. Occasional autophagic vacuoles containing clusters of melanosomes are also present. A few metastases from the transplanted melanoma have been observed in lymph nodes and on one occasion in the lungs. When grown in vitro, BULT melanoma cells do not require special growth promoting agents (e.g., TPA; cAMP) in order to proliferate. The BULT melanoma differs in one or more respects from each of the other three transplantable spontaneous mouse melanomas widely used in cancer research. In addition, it arose in a strain of mice characterized by the spontaneous death of melanocytes while the latter are engaged in synthesizing eumelanin within hair follicles. Karyotypic analysis of cultured cells showed a modal chromosome number of 68 with a range of 58–72 chromosomes. 相似文献
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RUTH HALABAN 《Pigment cell & melanoma research》1988,1(Z1):18-26
To proliferate in vitro, normal melanocytes, unlike normal fibroblasts, require specific growth factors in addition to those supplied in serum. The substances that promote melanocyte proliferation, such as 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and stimulators of cyclic adenosine monophosphate (cAMP), also promote pigmentation. Consequently, cell division and expression of at least some differentiated functions are not mutually exclusive for melanocytes. At present, the only known natural growth factor that can replace TPA in normal human melanocyte cultures is basic fibroblast growth factor (bFGF). Like TPA, bFGF is effective, most of the time, only in the presence of added cAMP. Some preparations of bFGF, however, may have a highly labile, intrinsinc cAMP stimulatory activity. It is thus possible that bFGF can assume two forms, dependent on and independent of cAMP stimulatory activity. Alternatively, a second factor may exist in pituitary glands that co-purifies with bFGF but deteriorates with storage. Abnormal melanocytes in culture, such as those derived from dysplastic nevi and primary melanomas, depend on the specific factors (bFGF and cAMP), whereas melanocytes from metastatic melanomas do not 相似文献
9.
JAMES H. JOHNSON 《Freshwater Biology》1985,15(6):749-755
SUMMARY.
- 1 Diel feeding periodicity and diet composition of nymphs of Aeshna multicolor and Lestes unguiculatus were examined at 6 h intervals in a small pond. Feeding intensity of A. multicolor increased from 06.00 hours to approximately 18.00 hours whereas food intake by L. unguiculatus was uniformly high between 12.00 and 18.00 hours. Feeding of both species was reduced during the night.
- 2 Diel variation in diet composition was substantial in each species. Chironomid larvae were the major prey (48.9%) in the diet of A. multicolor. Zooplankton, mainly cladocerans (42.0%) and ostracods (21.1%), were the principal prey of L. unguiculatus. Detritus composed a substantial portion of the diets of both species.
- 3 Diet overlap (Cλ) at each 6 h interval was high (0.43–0.72) when detritus was included in the calculation and was low (0.48–0.54) when detrital material was omitted.
10.
RICHARD K. JOHNSON 《Freshwater Biology》1985,15(5):605-612
SUMMARY.
- 1 Absorption efficiencies of nitrogen and carbon in two Chironomus species found dominating in the profundal zone of mesotrophic Lake Erken were determined gravimetrically.
- 2 Absorption efficiencies for C. plumosus showed greater seasonal variation than those of C. anthracinus, with low efficiencies coinciding with the summer dominance of flagellate phytoplankton and with high C:N ratios.
- 3 Overall mean absorption efficiencies (±SE) for carbon and nitrogen, respectively, were 26.8% (±1.9) and 29.3% (±1.9) for C. plumosus, 24.6% (±1.7) and 28.1% (±1.8) for C. anthracinus.
- 4 Significant differences were found to exist between the C: N ratios of the superficial 2 cm sediment layers and those of Chironomus anterior midgut contents.
- 5 C. anthracinus appears to be a deposit feeder ingesting particulate matter scraped from the recently deposited surface sediments. The greater seasonal variation found in the absorption efficiencies of C. plumosus, together with the lower C:N ratios, support the contention that this species is a filter feeder with the nutritional quality of ingested matter depending primarily on pelagic inputs.