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排序方式: 共有88条查询结果,搜索用时 15 毫秒
1.
SATORU KOBAYASHI HIDEKO MIZUNO MASUKICHI OKADA 《Development, growth & differentiation》1988,30(3):251-260
We describe the accumulation and distribution of poly (A)+ RNA during oogenesis and early embryogenesis as revealed by in situ hybridization with a radio-labeled poly (U) probe. The amount of poly (A)+ RNA in nurse cell cytoplasm continuously increased untill mid-vitellogenic stage (st. 10), then decreased with the rapid increase of poly (A)+ RNA in the oocyte (st. 11). The localization of poly (A)+ RNA at stage 10 was in the anterior region of the oocyte, where it is connected by cytoplasmic bridge to the nurse cells. These observations indicate that most of the poly (A)+ RNA synthesized in the nurse cells is transferred to the oocyte through the cytoplasmic bridges at stage 10–11. During the remainder of oogenesis (st. 11–14) and during preblastodermal embryogenesis, poly (A)+ RNA was evenly distributed over the cytoplasm of oocytes and embryos. At blastoderm stage, poly(A)+ RNA became concentrated in the peripheral region of embryos. Though the somatic nuclei of the blastoderm contained a detectable amount of poly (A)+ RNA, the pole cell nuclei did not. The cytoplasmic RNA visualised by acridine orange staining and the poly (A)+ RNA detected by hybridization with [3 H]poly (U) exhibited identical distributions during oogenesis and early embryogenesis. These observations provide a basis to assess the unique distributions of specific RNA sequences involved in early development. 相似文献
2.
Maternal Messenger RNA as a Determinant of Pole Cell Formation in Drosophila Embryos 总被引:1,自引:1,他引:0
Some polar plasm components are UV-sensitive. Messenger RNA extracted from oocytes or cleavage embryos can to induce pole cells in embryos that have been deprived of ability to form pole cells by UV-irradiation. This article reviews studies on the role of this mRNA in the developmental pathway leading to germ cell formation. 相似文献
3.
Histological Detection of Chicken δ-Crystallin DNA Sequences Introduced into Mouse Teratocarcinoma Cell Lines 总被引:1,自引:1,他引:0
YOSHIKO TAKAHASHI T. S. OKADA HISATO KONDOH 《Development, growth & differentiation》1985,27(5):607-613
In situ hybridization techniques to detect specific DNA sequences in histological sections were developed for the purpose of analyzing experimental chimeras produced by combination of mouse teratocarcinoma (TCC) cells stably carrying chicken δ-crystallin DNA sequences and normal mouse embryos. Various hybridization conditions for detection of exogenous DNA sequences were compared in samples of solid tumors of TCC lines. Of the conditions examined, denaturation of DNA in alkali and hybridization at 68°C in 6x SSC in the presence of dextran sulphate was the best for detecting δ-crystallin DNA sequences. With 3 H-labelled probe under these conditions, virtually all nuclei containing more than 100 copies of chicken δ-crystallin sequences were labelled sufficiently to be distinguishable from nuclei without chicken sequences. This technique could be applied to other experimental chimeras in which specific DNA sequences can be used as markers of certain cell lineages. 相似文献
4.
Embryos of Drosophila melanogaster at the early intravitelline nuclear multiplication stage were irradiated with UV light at the posterior pole. The sterility and mortality of these embryos were examined in relation to the dose and wavelength of the UV light.
Sterility, expressed either as the frequency of pole-cell-deficient embryos, or as the frequency of agametic adults, was found to be dependent on the wavelength of UV light. UV-irradiation at 280 nm was found moot effective in causing sterility on Drosophila embryos. The minimum dose of radiation to give a 100% sterility was 200 J/m2 at 280 nm, and 400 J/m2 at 254 nm. In contrast, mortality showed no dependency on the wavelength.
The possibility that nucleic acids in the posterior region is a target of 280 nm radiation is discussed. 相似文献
Sterility, expressed either as the frequency of pole-cell-deficient embryos, or as the frequency of agametic adults, was found to be dependent on the wavelength of UV light. UV-irradiation at 280 nm was found moot effective in causing sterility on Drosophila embryos. The minimum dose of radiation to give a 100% sterility was 200 J/m
The possibility that nucleic acids in the posterior region is a target of 280 nm radiation is discussed. 相似文献
5.
MASAHIRO SATO SHUN-ICHIROH OGATA RYUZO UEDA REIKO NAMIKAWA TOSHITADA TAKAHASHI TAKAO NAKAMURA EIICHI SATO TAKASHI MURAMATSU 《Development, growth & differentiation》1983,25(4):333-344
C-9-1, a monoclonal IgM antibody raised against human null cell acute lymphocytic leukemia cells reacted with restricted regions of embryonic and adult tissues of the mouse. The antigen positive sites in the embryos included embryonic ectoderm, visceral endoderm, trophoblastic cells invading the maternal decidua of 5∼7-day embryos, primordial germ cells of 10∼12-day embryos, epithelium of nasal chamber, the bronchus, Mullerian duct, epididymis and bladder of 12∼17-day embryos. In the adult mice, C-9-1 antigen was detected in renal tubules, a part of stomach, bladder, endometrium and epididymal sperm. Embryonal carcinoma cells, but not endodermal cells of teratocarcinoma expressed the antigen. Thus, C-9-1 antigen showed distribution similar to SSEA-1. However, C-9-1 antigen was not detected in preimplantation embryos, nor in oviduct, both of which are positive for SSEA-1. 相似文献
6.
Dissociated cells of lens epithelia of adult rats were monolayerly cultured in vitro. After about 15–20 days' period of active cell growth, such characteristic structures that correspond to "lentoid bodies" described previously in chick cultures were formed. These structures consisted of elongated cells, ultrastructural profile of which was similar with lens fiber. The presence of gamma-crystallin, a marker molecule specific to mature lens fiber, was confirmed in these elongated cells by means of fluorescent antibody technique. The differentiation of lens fiber in vitro was also recognized in clones originating from single lens epithelial cells cultured at very low cell density. 相似文献
7.
KIYOKAZU AGATA HISATO KONDOH SHIN TAKAGI KAZUYA NOMURA T. S. OKADA 《Development, growth & differentiation》1980,22(3):571-577
The effects of three different culture media (Eagle's MEM, F-12 and L-15) on the transdifferentiation of 8-day chick embryonic neural retina into lens cells, were examined with respect to the expression of two phenotypes. One type referred to neuronal specificity (as represented by the level of cholineacetyl-transferase, CAT, activity) and the other to lens specificity (as represented by content of α-and δ-crystallin). In 7-day cell cultures before the visible differentiation of lentoid bodies, CAT activity was detected in all media. But, its level was about 9 times higher in cultures with L-15 than in those with MEM and 3 times higher than in F-12. In 26-day cultures, CAT activity was practically undetectable. The production of α-and δ-crystallin was detected in cultures at 26 days. There were quantitative differences in the crystallin content with different media, and it was highest in cultures with L-15. The results indicate that conditions most favourable to the maintenance of the neuronal specificity in cell cultures of neural retina, can also support the most extensive transdifferentiation. The possibility of direct transdifferentiation of once neuronally specified cells into lens cells in cultures with L-15 has been suggested to explain the present results. 相似文献
8.
HOU-XIANG XIE MASATOSHI TAKEICHI SOH-ICHI OGOU T. S. OKADA 《Development, growth & differentiation》1982,24(5):513-520
An assay system was developed to detect a switch of mouse embryonal carcinoma (EC) cells to the pathway for normal cell differentiation after a brief contact with normal embryonic cells. The system consisted of (1) the mixed aggregation of AT805 EC cells with 8-cell stage mouse embryos, (2) the stationary culture of the mixed aggregates into blastocysts and (3) the cell culture of inner cell masses isolated from chimeric blastocysts containing EC cells at 2, 3 and 4 days after the initiaion of chimeric aggregation. The number of foci of EC cells which appeared in the cultures of inner cell masses was decreased with a length of contact of EC cells with normal embryos as the mixed aggregates. After 4 days' contact, only fibroblastic and epithelial cells appeared in most cultures of inner cell masses. Examination of isozyme markers of GPI revealed that such cell cultures consisting of nonmalignant cells contained cells of tumor origin. Thus, it was concluded that a brief exposure to the environment of normal embryos can regulate the tumor cells to differentiate into non-malignant cells. This conclusion was substantiated by comparing the pattern of protein spots of the tumor cells with that of non-malignant cells of the tumor origin by two dimensional gel electrophoresis. 相似文献
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