In vitro and in vivo discrepancy in inducing apoptosis by mesenchymal stromal cells delivering membrane-bound tumor necrosis factor–related apoptosis inducing ligand in osteosarcoma pre-clinical models

Background

Osteosarcoma (OS) is the most frequent pediatric malignant bone tumor. OS patients have not seen any major therapeutic progress in the last 30 years, in particular in the case of metastatic disease, which requires new therapeutic strategies. The pro-apoptotic cytokine Tumor necrosis factor (TNF)–Related Apoptosis Inducing Ligand (TRAIL) can selectively kill tumor cells while sparing normal cells, making it a promising therapeutic tool in several types of cancer. However, many OS cell lines appear resistant to recombinant human (rh)TRAIL-induced apoptosis. We, therefore, hypothesized that TRAIL presentation at the membrane level of carrier cells might overcome this resistance and trigger apoptosis.

Putting the Ninth Ward on the Map: Race, Place, and Transformation in Desire, New Orleans

In this article, we consider how long-term patterns of resistance to structural violence inform citizens' responses to displacement before and after Katrina. Drawing on Abdou Maliq Simone's (2004) conceptualization of people as infrastructure, we recenter the discussion about the rebuilding of New Orleans around displaced residents, taking the place-making practices of members of a social club as a lens through which to examine the predicament of the city as a whole. Members have been generating alternative ways of thinking about and dwelling together in a restructuring city. Their perspectives are articulated through in-depth interviews, focus groups, and the embodied practices of club members and their followers as they make claims to the city through massive, participatory street processions known as second lines. These distinctive ways of thinking and being in the city—the subaltern mainstream of the second-line tradition—are now being deployed by exiled New Orleanians reconsidering their relationship to home.     

The role of the blood meal in egg-laying periodicity and fecundity in Triatoma infestans

The number of eggs produced by Triatoma infestans is determined by the amount of blood ingested; the formation of one egg requires 16 mg blood. An exogenous supply of juvenile hormone, provided by the implantation of extra corpora aliata, increases egg production per unit of blood ingested. The corpus allatum is therefore implicated in the relationship between nutrition and fecundity. The rhythm of egg production is regulated by the nutritional state of the adult female: when the females are fed at frequent intervals, egg production becomes regular and continuous. This calls into question previous interpretations of egg-laying rhythms in the Triatominae as cyclic.     

Enzymatic Detachment of Endplate Acetylcholinesterase from Muscle

AT the vertebrate neuromuscular junction acetylcholinesterase catalyses the hydrolysis of the transmitter, acetylcholine, which is released from presynaptic nerve terminals^1,2. The enzyme is present in high concentration at the endplate, where it can be located by histochemical³ and autoradiographic⁴ methods. Electron microscopic studies of the endplate region show most of the histochemical reaction product to be in the synaptic cleft or associated with the nerve and muscle membranes^5–9. We report here that enzymatic treatment of intact muscle causes the detachment of active endplate acetylcholinesterase from the muscle into the bathing fluid.     

Anatomy and phylogenetic value of the mandibular and coronoid canals and their associated foramina in proboscideans (Mammalia)

Characters associated with the mandibular canal are differently distributed amongst proboscidean lineages and provide useful information on the systematics and relationships of proboscideans. The aim of this paper is to describe the pattern of the mandibular canal and its associated foramina in proboscideans in order to fully appreciate the extent of interspecific variation of these structures within the group and to discuss its systematic and phylogenetic value. Outgroup comparison indicates that the condition presented by the basal proboscidean Phosphatherium is morphotypic for proboscideans. Primitive proboscidean characters are: the low position of the mandibular foramen, and its crescent‐shaped outline, the occurrence of a coronoid foramen (canal), the occurrence of two lateral mental foramina, the posterior one at the level of (or slightly behind) the posterior margin of the symphysis, the anterior one in a more distal position, the absence of a medial mental foramen (MMF), the mandibular canal set just below the tooth row. The occurrence of a single lateral mental foramen may represent a shared derived character of Daouitherium, Numidotherium, and Barytherium. A unique derived feature of the Elephantinae mandible is the occurrence of a medial mental foramen on the medial side of the incisive part of the mandible. MMFs have never been observed in other proboscideans excluding elephantines. The very high frequency of MMFs observed in Mammuthus meridionalis–Mammuthus trogontherii–Mammuthus primigenius (>93 per cent of the studied specimens) could be considered a synapomorphy of this group. © 2010 The Linnean Society of London, Zoological Journal of the Linnean Society, 2011, 161 , 391–413.