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Development and use of anchored‐SSRs to study DNA polymorphism in bread wheat (Triticum aestivum L.)
RAVINDER SINGH NEERAJ KUMAR RAJIB BANDOPADHYAY SACHIN RUSTGI SHAILENDRA SHARMA HARINDRA SINGH BALYAN PUSHPENDRA KUMAR GUPTA 《Molecular ecology resources》2006,6(1):296-299
In bread wheat, 21 anchored simple sequence repeat (SSR) primer pairs detecting SSR length polymorphism and 42 anchored SSR primers detecting microsatellite‐anchored fragment length polymorphisms (MFLPs) are reported. Eight bread wheat genotypes were used for detecting polymorphism. The number of alleles in SSR analysis ranged from two to six, with a mean of 2.9 alleles per SSR. The number of polymorphic bands in MFLP ranged from two to 40, with a mean of 12.74 polymorphic bands/primer combination, the SSRs with CT/GA motifs giving the highest level of polymorphism (a mean of 18.37 bands). The average value of polymorphic information content (PIC) was 0.473 for SSRs and 0.061 for MFLP. 相似文献
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