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Elisa Quiala María-Jesús Cañal Mónica Meijón Roberto Rodríguez Maité Chávez Luis Valledor Manuel de Feria Raúl Barbón 《Plant Cell, Tissue and Organ Culture》2012,109(2):223-234
Shoot-tips, collected from greenhouse-grown plants of Tectona grandis L. (teak), were incubated on a semi-solid Murashige and Skoog (MS) medium with 2% (w/v) sucrose, and supplemented with 4.44 μM
6-benzyladenine (BA). These were then transferred to a temporary immersion system (TIS) using liquid MS medium supplemented
with 0 (CK-free medium), 2.22, 4.44, 6.66 μM BA. High mean numbers of shoots per explant were obtained when explants were
grown on medium containing either 4.44 or 6.66 μM BA and yielding 7.7 and 10.3 normal shoots (NS)/explant, respectively. Moreover,
these high BA levels contributed to lower accumulation of phenolic compounds and deposition of lignin in vascular cells of
the teak shoots following histochemical analysis. Morphological analysis of proliferating shoots by scanning microscopy revealed
that leaves of shoots incubated on either CK-free medium, 2.22, or 4.44 μM BA had elliptical stomata; whereas, stomata of
leaves of shoots grown on medium containing 6.66 μM BA were primarily ring-shaped, raised, and open. Moreover, misshapen stomata
with broken epidermal layers of guard cells, typical of hyperhydric leaves, were also observed. When shoots were rooted ex
vitro by dipping in 492.1 μM indole-3-butyric acid (IBA) for 2 min, the frequency of rooting of shoots previously grown on
either CK-free medium or 2.22 μM BA (96.7 and 91.7%, respectively) was higher than that of shoots grown on semi-solid medium
(73%). Shoots from both TIS treatments developed good root systems, and all plantlets (100%) survived transfer to soil mix
and acclimatization in the greenhouse. Plantlets established from shoots grown on 6.66 μM BA showed the lowest frequency of
survival (60%). After 3 months, plants were transferred to field conditions. 相似文献
2.
de Feria Manuel Jiménez Elio Barbón Raúl Capote Alina Chávez Maité Quiala Elisa 《Plant Cell, Tissue and Organ Culture》2003,72(1):1-6
The effect of two different dissolved oxygen (DO) concentrations (50 and 80%) on differentiation of somatic embryos (SE) from cell suspensions of coffee (Coffea arabica cv. Catimor 9722) was analyzed. Two bioreactors CMF-100 (CHEMAP AG) designed for the culture of cells, with 2-l glass vessels and a maximum work volume of 1.8 l were used. Each one was equipped with a gas blending unit (air, O2, N2, CO2) for the control of DO concentration. The inoculation density of embryogenic cells was 1.0 gram of fresh weight per liter (g FW l–1). The number of somatic embryos was greater (71 072 SE l–1) with 80% DO, but the major proportion were globular and heart shaped SE (66 399 SE l–1) and only 6.6% with regard to total was torpedo shaped SE. However, the 50% DO produced the higher number in the torpedo shaped SE (7389 SE l–1) what represented 20.0% with regard to total. Thus, higher concentrations of DO induced globular and heart shaped SE differentiation, but for production of torpedo shaped SE lower concentrations DO are needed. The somatic embryos obtained in the bioreactor with 50% DO showed similar behavior to the somatic embryos obtained in the rotary shaker. After 8 weeks of culture, 49.2% germination was obtained, which allowed a total of 1725 plantlet to be transferred to conditions ex vitro. After 6 months of culture, 89.2% of conversion was achieved and 1539 plants obtained were transferred to field conditions. 相似文献
3.
Somatic embryogenesis of the banana hybrid cultivar FHIA-18 (AAAB) in liquid medium and scaled-up in a bioreactor 总被引:2,自引:0,他引:2
Gómez Kosky Rafael de Feria Silva Manuel Posada Pérez Laisyn Gilliard Terrence Bernal Martínez Francisco Reyes Vega Maritza Chávez Milian Maité Quiala Mendoza Elisa 《Plant Cell, Tissue and Organ Culture》2002,68(1):21-26
The development of somatic embryos in liquid culture medium has a number of advantages for large-scale propagation of plants. This paper describes an improved system for the mass propagation via somatic embryogenesis of the banana hybrid cultivar FHIA-18 (AAAB). Explants from immature male flowers were used to form high frequency embryogenic tissue, this tissue was then used to establish embryogenic cell suspensions in a basic MS medium plus 1.0 mg l–1 biotin, 100 mg l–1 glutamine, 100 mg l–1 malt extract (Sigma), 1.0 mg l–1 2,4-D and 45 g l–1 sucrose. Secondary multiplication of somatic embryos was achieved in liquid media in rotary shaker and in bioreactors. The number of embryos per litre obtained with 80.0% DO2 and effects of pH were also studied. A high regeneration percentage of plants were obtained (89.3%) in only 1 month of culture, somatic embryos were then placed to germinate in temporary immersion systems and field testing of somaclonal variation. 相似文献
4.
Jiménez Elio Pérez Naivy de Feria Manuel Barbón Raúl Capote Alina Chávez Maité Quiala Elisa Pérez Juan C. 《Plant Cell, Tissue and Organ Culture》1999,59(1):19-23
A temporary immersion system for potato microtuber production was designed using 4-l vessels. This culture technique showed
several advantages compared to solid cultures: i.e., three fold increase in shoot length, more internodes per plant and improved
vigor. In the tuber induction stage, microtubers can be induced at all plant nodes, indicating that the tuberization is not
restricted to specific regions. For both cultivars tested, Desiree and Atlantic, an average of 3.1 and 2.8 tubers per single
node cutting was achieved after 9 weeks in culture. The size and weight of the tubers were higher than on solid media. Scale
up was performed with cv. Atlantic in 10-l polycarbonate flasks and 12 units were mounted containing 150 single nodal cuttings
each. An average of 2.6 tubers per inoculated cutting was obtained, with 1.3 g fresh weight per microtuber. Temporary immersion
is a valuable option for potato microtuber production, as well as for shoot production during the planting season.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
5.
Tectona grandis L. (teak) is one of the premier hardwood timbers in the world, ranking at present in the top five tropical hardwood species in terms of worldwide plantation area. Characterization of the proteins present in teak leaves will provide a basis for the development of new tools aimed at assisting tree selection, the monitoring of plant propagation, and the certification of clonal and phenotypic identities. In this paper, we describe the extraction, separation, and identification of leaf proteins from T. grandis using a TCA/acetone protocol, 2DE, and MALDI-TOF. After TCA/acetone protein extraction of leaves, 998 well-resolved spots were detected in Coomassie-stained gels within the 10-114 kDa relative molecular mass (Mr) range at a pH ranging from 3 to 11. A total of 120 spots were digested and subjected to MS. Of these, 100 nonredundant protein species were successfully identified. Functional classification of the identified proteins revealed that proteins involved in photosynthesis, protein translation, and energy production were the most abundant. This work is the first high-throughput attempt to study the T. grandis leaf proteome and represents a stepping stone for further differential expression proteomic studies related to growth, development, biomass production, and culture-associated physiological responses. 相似文献
6.
Tapia A Cheel J Theoduloz C Rodríguez J Schmeda-Hirschmann G Gerth A Wilken D Jordan M Jiménez-González E Gomez-Kosky R Mendoza EQ 《Zeitschrift für Naturforschung. C, Journal of biosciences》2007,62(5-6):447-457
The biomass production of Cymbopogon citratus shoots cultivated in bioreactors according to the temporary immersion (TIS) principle was assessed under different growth conditions. The effect of gassing with CO2-enriched air, reduced immersion frequency, vessel size and culture time on total phenolic and flavonoid content and free radical scavenging effect of the methanolic extracts was measured. From the TIS-culture of C. citratus, seven compounds were isolated and identified as caffeic acid (1), chlorogenic acid (2), neochlorogenic acid (3), p-hydroxybenzoic acid (4), p-hydroxybenzoic acid 3-O-beta-D-glucoside (5), glutamic acid (6) and luteolin 6-C-fucopyranoside (7). The occurrence of compounds 1-7 and their variability in C. citratus grown under different TIS conditions was determined by HPLC. The free radical scavenging effect of the methanolic extract and compounds was measured by the discoloration of the free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH). The main metabolites in 6- and 8-week-old cultures, both in 5 and 10 1 vessels, were chlorogenic acid (2) (100-113 mg%) and neochlorogenic acid (3) (80-119 mg%), while in the cultures with CO2-enriched air and reduced immersion frequency the main compound detected in the extracts was glutamic acid (6) (400 and 670 mg% for the green and white biomass and 619 and 630 mg% for the green and white biomass, respectively). The most active compounds, as free radical scavengers, in the DPPH discoloration assay were caffeic acid (1), chlorogenic acid (2), neochlorogenic acid (3) and the flavonoid luteolin 6-C-fucopyranoside (7). 相似文献
7.
Elisa Quiala Raul Barbón Elio Jimenez Manuel De Feria Maité Chávez Alina Capote Naivy Pérez 《In vitro cellular & developmental biology. Plant》2006,42(3):298-300
Summary
In vitro plants of lemon grass were established, starting from shoot apices derived from plants cultivated under field conditions.
The effect of the immersion frequency (two, four, and six immersions per day) on the production of biomass in temporary immersion
systems (TIS) of 1 liter capacity was studied. The highest multiplication coefficient (12.3) was obtained when six immersions
per day were used. The maximum values of fresh weight (FW; 62.2 and 66.2 g) were obtained with a frequency of four and six
immersions per day, respectively. However, the values for dry weight (DW; 6.4g) and height (8.97cm) were greater in the treatment
with four immersions per day. The TIS used in this work for the production of lemon grass biomass may offer the possibility
of manipulating the culture parameters, which can influence the production of biomass and the accumulation of secondary metabolites.
We describe for the first time the in vitro production of Cymbopogon citratus biomass in TIS. 相似文献
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