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1.
血管紧张素Ⅱ对模拟缺血心室肌细胞L-型钙通道的影响 总被引:3,自引:0,他引:3
实验研究了血管紧张素II(AngⅡ)对模拟缺血心室肌细胞L-型钙离子通道的作用,用胶原酶酶解法急性分离豚鼠心室肌细胞,以全细胞膜片钳方法记录心室肌细胞的L-型钙电流(ICa L.)。采用低氧,无糖,高乳酸和酸中毒综合方式模拟缺血液灌流,造成心室肌细胞的模拟缺血,并在缺血的基础上继续用含100mmol/A AngⅡ灌流细胞,观察AngⅡ对模拟缺血心室肌细胞钙离子通道的影响,实验结果显示,模拟缺血时ICa.L峰值电流明显减小,最大激活电压为0mV,AngⅡ能抵抗模拟缺血对ICa,L的抑制效应,使ICa,L峰值电流增大,并使最大激活电压左移至-10mV。 相似文献
2.
Jian-Zhong Liu Qian-Ling Zhang Weng Li-Ping Ji Liang-Nian 《Applied Biochemistry and Microbiology》2003,39(5):493-496
The Aspergillus niger strain ZBY-7 was selected as the original strain of glucose-6-phosphate dehydrogenase production. After mutagenesis of the strain by means of UV irradiation and nitrosoguanidine, mutants of Aspergillus niger resistant to a certain metabolic inhibitor were obtained. Five of the mutants showed increased glucose-6-phosphate dehydrogenase production. The mutant resistant to antimycin A (Aspergillus niger AM-23) produced the highest level of glucose-6-phosphate dehydrogenase (695.9% of that produced by the original strain). 相似文献
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不同桑树品种上朱砂叶螨实验种群内禀增长率的统计推断 总被引:8,自引:0,他引:8
为了正确地评价不同桑树品种上朱砂叶螨为害差异,本文通过使用Jackknife方法(刀切法),采用合适的生殖力生命表构建方法,对朱砂叶螨实验种群在不同的4个桑树品种上生殖力表的内禀增长率进行了统计推断。结果表明,温度为28±1℃、相对湿度75%±10%、光周期16L∶8D的条件下,西农6071 (山桑 Morus bombycis Koidz.,2x)、和田白桑 (白桑 M. alba Linne, 3x)、新一之濑 (白桑 M. alba Linne, 2x)和大石(广东桑 M. atropurpurea Roxb., 3x) 4个桑树品种上朱砂叶螨rm值大小依次为0.41894(0.41043~0.42746)、0.37065(0.36604~0.37526)、0.36171(0.35778~0.36563)和0.35253(0.34757~0.35748)。通过对“伪值”样本同秩的成对t检验比较,这4个桑树品种对朱砂叶螨内禀增长率的影响有显著差异。结果说明朱砂叶螨对4种桑树的易感性,以西农6071、和田白桑、新一之濑、大石顺序渐弱。 相似文献
4.
The strain of Aspergillus niger ZBY-7 was selected as the original strain of glucose 6-phosphate dehydrogenase production. After mutagenesis of the strain using UV irradiation and nitrosoguanidine, mutants of Aspergillus niger resistant to certain metabolic inhibitor were obtained. Five of the mutants showed increased glucose 6-phosphate dehydrogenase production. The mutant resistant to antimycin A (Aspergillus niger AM-23) produced the highest level of glucose 6-phosphate dehydrogenase (695.9% of that from the original strain). 相似文献
5.
The design of new molecular "light switches" for DNA 总被引:6,自引:0,他引:6
Xiao-Hua Zou Bao-Hui Ye Hong Li Qian-Ling Zhang Hui Chao Jin-Gang Liu Liang-Nian Ji Xiao-Yuan Li 《Journal of biological inorganic chemistry》2001,6(2):143-150
Two novel ruthenium(II) complexes, [Ru(pztp)2(phen)](ClO4)2 and [Ru(pztp)2(bpy)] (ClO4)2, have been synthesized and characterized by UV/Vis and 1H NMR spectroscopies and mass spectrometry. The MeCN solutions of both complexes display fluorescence that was found to be highly sensitive to the presence and concentration of water. The complexes behave like a "light switch" for DNA in that they do not luminesce in water but were "turned on" in the presence of DNA and show emission enhancement with the increase of DNA concentration. Their DNA binding behavior was also studied by absorption spectroscopy and viscosity measurements, which suggest that the DNA-complex interaction involves intercalation of the metal-bound pztp ligand into the base pairs of duplex DNA. 相似文献
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血管紧张素Ⅱ对缺血心肌细胞钾离子通道的作用 总被引:5,自引:0,他引:5
实验用胶原酶酶解法急性分离豚鼠心室肌细胞,利用全细胞膜片钳的方法记录心室肌细胞的延迟整流钾电流(Ik)、内向整流钾电流(Ik1)和ATP敏感钾电流(IKATP)。采用低氧、无糖、高乳酸和酸中毒综合方式模拟缺血灌流,造成细胞的模拟缺血,并在缺血的基础上继续用含100nmol/L AngⅡ灌流细胞,观察Ang Ⅱ对模拟缺血心室肌细胞钾离子通道的影响。实验结果显示:(1)模拟缺血时,Ik明显减小;Ang Ⅱ能进一步抑制Ik。(2)模拟缺血条件下,Ik1受到抑制,并且以内向电流的抑制为主;Ang Ⅱ可加强对Ik1内向电流的抑制,而对部分外向电流则有增加的作用。(3)模拟缺血使IKATP外向电流略有增加;Ang Ⅱ则明显加强IKATP外向电流,此效应能被优降糖所阻断。 相似文献
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Zhang QL Liu JH Ren XZ Xu H Huang Y Liu JZ Ji LN 《Journal of inorganic biochemistry》2003,95(2-3):194-198
The ligand ODHIP (3,4-dihydroxyl-imidazo[4,5-f][1,10]phenanthroline) and its cobalt(III) complex [Co(bpy)(2)(ODHIP)](3+) were synthesized and characterized. Binding of this complex with calf thymus DNA has been investigated by spectroscopic methods and viscosity. The experimental results indicated that the complex bound to DNA by intercalation. In Tris buffer, the complex could emit relatively weak luminescence. After binding to DNA, the notable enhancement was observed. However, when the Cu(2+) was further added, the luminescence decreased gradually and disappeared after the equimolar concentrations of Cu(2+) was added, which exhibited the "off-on-off" properties of molecular light switch. 相似文献
10.
Zhang QL Liu JH Liu JZ Zhang PX Ren XZ Liu Y Huang Y Ji LN 《Journal of inorganic biochemistry》2004,98(8):1405-1412
A series of enantiomeric polypyridyl ruthenium(II) complexes Delta- and Lambda-[Ru(bpy)2CNOIP](PF6)2 (Delta-1 and Lambda-1; BPY=2,2'-bipyridine, CNOIP=2-(2-chloro-5-nitrophenyl)imidazo[4,5-f][1,10]phenanthroline), Delta- and Lambda-[Ru(bpy)2HPIP](PF6)2 (Delta-2 and Lambda-2; HPIP=2-(2-hydroxyphenyl)imidazo[4,5-f][1,10]phenanthroline), Delta- and Lambda-[Ru(bpy)2DPPZ](PF6)2 (Delta-3 and Lambda-3; DPPZ=dipyrido[3,2:a-2',3':c]-phenazine), Delta- and Lambda-[Ru(bpy)2TAPTP](PF6)2 (Delta-4 and Lambda-4; TAPTP=4,5,9,18-tetraazaphenanthreno[9,10-b] triphenylene) have been synthesized. Binding of these chiral complexes to calf thymus DNA has been studied by spectroscopic methods, viscosity, and equilibrium dialysis. The experimental results indicated that all the enantiomers of these complexes bound to DNA through an intercalative mode, but the binding affinity of each chiral complex to DNA was different due to the different shape and planarity of the intercalative ligand. After binding to DNA, the luminescence property of complex 1 was distinctly different from complexes 2 to 4. Upon irradiation at 302 nm, complexes 2-4 were found to promote the cleavage of plasmid pBR 322 DNA from supercoiled form I to nicked form II, and obvious enantioselectively was observed on DNA cleavage for the enantiomers of complexes 2 and 4. The mechanisms for DNA cleavage by these enantiomeric complexes were also proposed. 相似文献