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A gentle method for the isolation of nuclei from developing silk glands ofBombyx mori has been standardized. The nuclei, whether isolated or directly visualizedin situ within the silk glands, exhibit complex morphology. The nuclei occupy almost the entire volume of the gigantic silk gland
cells. Although the isolated nuclei still retain their ramified morphology, being polyploid they are fragile and often become
fragmented. The histone and low-salt-extractable proteins from nuclei isolated from the middle and posterior silk glands on
different days of the fourth and fifth instars of larval development have been analysed. The histones did not show any stage-
or tissue-specific variations whereas the low-salt-extractable proteins showed some developmental stage specific variation.
Using the antibody raised against one such protein, its absence in the early stage of development has been confirmed by Western
blotting techniques. This developmental stage specific protein may be functionally linked to some activities responsible for
boosting up the production of silk or silk-related proteins during the fifth instar of larval development. 相似文献
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Sisinthy Shivaji N. Shyamala Rao L. Saisree Vipula Sheth G. S. N. Reddy Pushpa M. Bhargava 《Journal of biosciences》1988,13(4):409-414
Five cultures isolated from soil samples collected in Schirmacher oasis, Antarctica, have been identified as members of the
familyMicrococcaceae, with 3 belonging to the genusMicrococcus and two toPlanococcus. The 3Micrococcus isolates (37R, 45R and 49R) were red-pigmented and h a d ∼ 75 mol% G + C in their DNA; they were identified asMicrococcus roseus. The twoPlanococcus isolates (30Y and Lz3OR) were yellow and orange in colour, and had 43.5 and 40.9 mol % G + C in their DNA respectively; they
were identified asPlanococcus sp. 相似文献
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T. G. Krishna S. E. Pawar R. Mitra 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1986,73(1):82-87
Summary Variation in the arachin polypeptides of groundnut genotypes was observed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Three regions could be observed on the electropherogram. Region 1, corresponding to conarachin, did not show any variation; region 2, consisting of arachin acidic subunits, showed variation; region 3, containing the arachin basic subunits, did not show any variation. There are four varietal classes of arachin polypeptide patterns: class A comprised three acidic subunits of arachin of molecular weights 47.5, 45.1 and 42.6 kd and a basic subunit of 21.4 kd; class B, with three acidic subunits of molecular weights 47.5, 45.1 and 41.2 kd and a basic subunit of 21.4 kd; class C of an additive pattern of class A and class B; class D, of two acidic polypeptides of 47.5, 45.1 kd and the basic 21.4 kd subunit. Of the 90 genotypes studied, 73% belong to class A, 15% to class B and 6% each to class C and D. Analysis of F2 seeds from a cross between class A and class B genotypes showed that the two polypeptides (42.6 kd and 41.2 kd) are coded by nonallelic genes and also revealed that class C and class D patterns arose as a result of hybridisation between class A and class B. A. monticola, the progenitor of A. hypogaea, showed a pattern similar to the additive pattern of class A and class B while some diploid Arachis species had the 41.2 kd polypeptide. Based on arachin polypeptide patterns the probable origin of A. hypogaea has been suggested. 相似文献
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A case of somatic instability affecting aleurone colour in a strain of maize from India with flint background was analysed.
The somatic instability is localized to theC
1 (Inhibitor) allele ofC locus on the short arm of chromosome 9. Molecular tests indicated thatAc is not present in the Indian stock and the evidence is consistent with the involvement of theEn (Spm) transposable element in the instability. The presence of theEn (Spm)-like element in the stock would suggest that these elements have been present in the maize genome for a long time. A new
allele ofshrunken (sh1) gene with a somewhat unorthodox breeding behaviour is also described. 相似文献
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Madar Jyoti M. Samundeeswari S. Holiyachi Megharaja Naik Nirmala S. Pawar Varsha Gudimani Parashuram Shastri Lokesh A. Kumbar Vijay M. Sunagar Vinay A. 《Russian Journal of Bioorganic Chemistry》2021,47(2):535-542
Russian Journal of Bioorganic Chemistry - The present work highlights the broad range of oxygen and nitrogen heterocycles and their applications in medicinal field. A facial approach has been... 相似文献
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Aminopeptidase B, an arginyl aminopeptidase, was purified from goat brain with a purification factor of ~280 and a yield of 2.7%. It was entrapped in calcium alginate together with bovine serum albumin. The optimal conditions for immobilization for maximum activity yield were 1% CaCl2 and 2.5% alginate. The immobilized enzyme retained ~62% of its initial activity and could be used for five successive batch reactions with retention of 30% of the initial activity. The pH and temperature optima of the free and immobilized enzyme were pH 7.4, 45°C and pH 7.8, 50°C respectively, while the pH and thermal stability as well as the stability of the enzyme in organic solvents were improved significantly after entrapment. The Km value for the immobilized enzyme was about twofold higher than that of the soluble enzyme. Because of this increased stability, the immobilized enzyme may be useful in the meat processing industry. 相似文献