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EA Dukhanina TI Lukyanova EA Romanova V Guerriero NV Gnuchev GP Georgiev DV Yashin LP Sashchenko 《Cell cycle (Georgetown, Tex.)》2015,14(22):3635-3643
PGRP-S (Tag7) is an innate immunity protein involved in the antimicrobial defense systems, both in insects and in mammals. We have previously shown that Tag7 specifically interacts with several proteins, including Hsp70 and the calcium binding protein S100A4 (Mts1), providing a number of novel cellular functions. Here we show that Tag7–Mts1 complex causes chemotactic migration of lymphocytes, with NK cells being a preferred target. Cells of either innate immunity (neutrophils and monocytes) or acquired immunity (CD4+ and CD8+ lymphocytes) can produce this complex, which confirms the close connection between components of the 2 branches of immune response. 相似文献
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Ylianttila MS Pursiainen NV Haapalainen AM Juffer AH Poirier Y Hiltunen JK Glumoff T 《Journal of molecular biology》2006,358(5):1286-1295
(3R)-hydroxyacyl-CoA dehydrogenase is part of multifunctional enzyme type 2 (MFE-2) of peroxisomal fatty acid beta-oxidation. The MFE-2 protein from yeasts contains in the same polypeptide chain two dehydrogenases (A and B), which possess difference in substrate specificity. The crystal structure of Candida tropicalis (3R)-hydroxyacyl-CoA dehydrogenase AB heterodimer, consisting of dehydrogenase A and B, determined at the resolution of 2.2A, shows overall similarity with the prototypic counterpart from rat, but also important differences that explain the substrate specificity differences observed. Docking studies suggest that dehydrogenase A binds the hydrophobic fatty acyl chain of a medium-chain-length ((3R)-OH-C10) substrate as bent into the binding pocket, whereas the short-chain substrates are dislocated by two mechanisms: (i) a short-chain-length 3-hydroxyacyl group ((3R)-OH-C4) does not reach the hydrophobic contacts needed for anchoring the substrate into the active site; and (ii) Leu44 in the loop above the NAD(+) cofactor attracts short-chain-length substrates away from the active site. Dehydrogenase B, which can use a (3R)-OH-C4 substrate, has a more shallow binding pocket and the substrate is correctly placed for catalysis. Based on the current structure, and together with the structure of the 2-enoyl-CoA hydratase 2 unit of yeast MFE-2 it becomes obvious that in yeast and mammalian MFE-2s, despite basically identical functional domains, the assembly of these domains into a mature, dimeric multifunctional enzyme is very different. 相似文献
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Predation by signal crayfish Pacifastacus leniusculus on fish eggs and its consequences for coregonid recruitment 下载免费PDF全文
J. Karjalainen T. J. Ruokonen T. J. MarjomäKi A. Martikainen M. Pursiainen J. Sarvala M. Tarvainen A.‐M. Ventelä 《Journal of fish biology》2015,86(2):651-667
The character and magnitude of predation by the invasive, ectothermic Pacifastacus leniusculus, a crayfish widely introduced to Europe and Japan from North America, on the eggs of coregonid fishes, vendace Coregonus albula and whitefish Coregonus lavaretus were examined by experimentation, modelling and field data. The present results showed that P. leniusculus has the potential to be very efficient predator of fish eggs under winter conditions, but the predation by P. leniusculus did not significantly decrease production of coregonid larvae during the years with a high P. leniusculus population in the study lake. Hence, the mortality caused by the novel invertebrate predator appeared to compensate for other yet unexplored mortality factors instead of having an additive effect on the present salmonids. 相似文献
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We present an efficient computational architecture designed using supervised machine learning model to predict amyloid fibril
forming protein segments, named AmylPepPred. The proposed prediction model is based on bio-physio-chemical properties of
primary sequences and auto-correlation function of their amino acid indices. AmylPepPred provides a user friendly web interface
for the researchers to easily observe the fibril forming and non-fibril forming hexmers in a given protein sequence. We expect that
this stratagem will be highly encouraging in discovering fibril forming regions in proteins thereby benefit in finding therapeutic
agents that specifically aim these sequences for the inhibition and cure of amyloid illnesses.
Availability
AmylPepPred is available freely for academic use at www.zoommicro.in/amylpeppred 相似文献7.
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Timo?J.?RuokonenEmail author Juha?Karjalainen Mikko?Kiljunen Markku?Pursiainen Heikki?H?m?l?inen 《Biological invasions》2012,14(4):813-825
Invasive crayfish are spreading rapidly across Europe, where they are replacing the native crayfish species and impacting
negatively on some other biota. Freshwater crayfish and many benthic fishes share similar habitat and food requirements and
hence potentially compete for resources. In this study, we investigated impacts of the introduced signal crayfish (Pacifastacus leniusculus) on fish in stony littoral habitats of two large boreal lakes. We compared the littoral fish community composition and the
densities of two common benthic fish species between sites with and without crayfish. To evaluate whether signal crayfish
share the same food resources as benthic littoral fish or change their feeding habits, we used mixing models and trophic niche
estimates based on analyses of stable isotopes of carbon and nitrogen. Both the community composition of littoral fish and
the densities of benthic fish species were similar at sites with and without signal crayfish. Even though stable isotope signatures
indicated strong dietary overlap between crayfish and benthic fish, the use of food sources and trophic niche widths of fish
were not noticeably different between crayfish sites and non-crayfish sites. Our results suggest that, at current densities,
the non-native signal crayfish does not have significant impacts on benthic fish in the stony littoral habitats of large boreal
lakes. 相似文献
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Cyclooxygenases (COXs) catalyze the rate-limiting step in the production of prostaglandins, bioactive compounds involved in
processes such as fever and sensitivity to pain, and are the target of aspirin-like drugs. COX genes have been cloned from coral, tunicates and vertebrates, and in all the phyla where they are found, there are two genes
encoding two COX isoenzymes; it is unclear whether these genes arose from an early single duplication event or from multiple
independent duplications in evolution. The intron-exon arrangement of COX genes is completely conserved in vertebrates and mostly conserved in all species. Exon boundaries largely define the four
functional domains of the encoded protein: the amino-terminal hydrophobic signal peptide, the dimerization domain, the membrane-binding
domain, and the catalytic domain. The catalytic domain of each enzyme contains distinct peroxidase and cyclooxygenase active
sites; COXs are classified as members of the myeloperoxidase family. All COXs are homodimers and monotopic membrane proteins
(inserted into only one leaflet of the membrane), and they appear to be targeted to the lumenal membrane of the endoplasmic
reticulum, where they are N-glycosylated. In mammals, the two COX genes encode a constitutive isoenzyme (COX-1) and an inducible isoenzyme (COX-2); both are of significant pharmacological
importance. 相似文献
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Sabaté M Ligthart J Deshpande N DeFeyter P Serruys P 《International journal of cardiovascular interventions》1998,1(2):109-112
We report a case of implantation of a new design of stent which allows creation of a double-hemispheric lumen for the treatment of a bifurcational stenosis. The unfavourable outcome following the implantation of this stent is described. 相似文献