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1.
Wang R Hanna MA Zhou WW Bhadury PS Chen Q Song BA Yang S 《Bioresource technology》2011,102(2):1194-1199
A comparative study on the composition, biodiesel production and fuel properties of non-edible oils from Euphorbia lathyris L. (EL), Sapium sebiferum L. (SS), and Jatropha curcas L. (JC) was conducted. Under optimal conditions, the FAME content and yield of the three oils were greater than 97.5 wt.% and 84.0%, respectively. The best biodiesel was produced from EL due to its high monounsaturation (82.66 wt.%, Cn: 1), low polyunsaturation (6.49 wt.%, Cn: 2, 3) and appropriate proportion of saturated components (8.78 wt.%, Cn: 0). Namely, EL biodiesel possessed a cetane number of 59.6, an oxidation stability of 10.4 h and a cold filter plug point of -11 °C. However, the cetane number (40.2) and oxidative stability (0.8 h) of dewaxed SS kernel oil (DSSK) biodiesel were low due to the high polyunsaturation (72.79 wt.%). In general, the results suggest that E. lathyris L. is a promising species for biodiesel feedstock. 相似文献
2.
Xin-Hua Liu Hui-Feng Liu Xu Shen Bao-An Song Pinaki S. Bhadury Hai-Liang Zhu Jin-Xing Liu Xing-Bao Qi 《Bioorganic & medicinal chemistry letters》2010,20(14):4163-4167
A series of novel 2-chloro-pyridine derivatives containing flavone, chrome or dihydropyrazole moieties as potential telomerase inhibitors were synthesized. The bioassay tests showed that compounds 6e and 6f exhibited some effect against gastric cancer cell SGC-7901 with IC50 values of 22.28 ± 6.26 and 18.45 ± 2.79 μg/mL, respectively. All title compounds were assayed for telomerase inhibition by a modified TRAP assay, the results showed that compound 6e can strongly inhibit telomerase with IC50 value of 0.8 ± 0.07 μM. Docking simulation was performed to position compound 6e into the active site of telomerase (3DU6) to determine the probable binding model. 相似文献
3.
Nematodes form an important component of many benthic marine ecosystems and DNA barcoding approaches could provide an insight
into nematode community composition from different environments globally. We have amplified nematode 18S rRNA sequences using
standard nematode18S rRNA primers from environmental DNA extracted from intertidal sediment collected from New Jersey coast,
USA to test whether the published marine nematode 18S rRNA sequences from GenBank and EMBL databases can effectively assign
unknown nematode sequences into genus or species level. Most of the sequenced clones showed some degree of identities with
published marine nematode 18S rRNA sequences. However, relatively very few of the sequences could be assigned even to genus
level based on sequence assignment rule. In addition, other eukaryotic 18S rRNA sequences were found to be co-amplified with
commonly used nematode 18S rRNA primers. We found that the majority of the current nematode 18S rRNA primers will co-amplify
other eukaryotes if environmental DNA is the target template. We therefore designed a new set of nematode 18S rRNA primers
and evaluated them using environmental DNA in intertidal sediment from the New Jersey coast. In total, 40 clones were screened
and subsequently sequenced and all the sequences showed varying degree of identities with published nematode 18S rRNA sequences
from GenBank and EMBL databases, and no obvious eukaryotic co-amplicons were detected with new primers. Only 13 out of 40
clones amplified with the new primer set showed 100% identity to published Daptonema and Metachromadora 18S rRNA sequences. The current molecular databases for nematodes are dominated by sequences from NW Europe and need to be
more extensively populated with new full length 18S rRNA nematode sequences collected from different biogeographic locations.
The new primers developed in this study, in combination with an updated nematode 18S rRNA sequence database, would help us
to better investigate and understand the diversity and community composition of free-living marine nematodes based on DNA
barcoding approaches during biodiversity or biomonitoring surveys on a global-scale. 相似文献
4.
Distribution patterns of marine planktonic cyanobacterial assemblages in transitional marine habitats using 16S rRNA phylogeny
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The community composition of marine planktonic cyanobacteria in transitional marine habitats can influence its overall contribution to aquatic primary production. To understand distribution patterns of marine planktonic cyanobacterial assemblages, phylogenetic and statistical analyses were undertaken on planktonic cyanobacterial 16S rRNA gene sequences from four transitional marine habitats [Baltic Sea (BL), Monterey Bay (MB), South China Sea (SCS) and Sundarbans (SB)]. Out of 3255 sequences analyzed, only 546 sequences were found to be planktonic cyanobacteria and were considered in this study. Among these, 338 sequences representative of Sundarbans, the world's largest mangrove were generated based on Sanger and Illumina sequencing approaches. Based on 16S rRNA phylogeny, four major taxonomic orders of marine planktonic cyanobacteria were recovered in varying proportions with several novel 16S rRNA sequences in each of the four targeted sites. Members of the order Synechococcales were dominant in all the sites (?94% sequences) while the orders Chroococcales and Oscillatoriales were only detected in SB and SCS sites, respectively. In the phylogenetic tree, sequences representing the major marine picocyanobacterial genus Synechococcus showed overwhelming dominance in SB and they were found in three other sites. Prochlorococcus ‐like sequences were found in sizeable number in MB and SCS but were absent in SB and coastal BL. Synechococcus ‐like sequences were represented by three major marine clusters (5.1, 5.2, and 5.3). Three novel clades as part of Synechococcus cluster were detected only in SB and one novel clade in BL. The majority of OTUs were found to be exclusive to each site, whereas some were shared by two or more sites as revealed by beta‐diversity analysis. 相似文献
5.
Liu XH Cui P Song BA Bhadury PS Zhu HL Wang SF 《Bioorganic & medicinal chemistry》2008,16(7):4075-4082
A series of novel 1-(5-substituted-3-substituted-4,5-dihydropyrazol-1-yl)ethanone oxime ester derivatives are synthesized. The results show that compounds 14 and 26c can strongly inhibit Staphylococcus aureus DNA gyrase and Escherichia coli DNA gyrase (with IC(50) of 0.25 and 0.125 microg/mL against S. aureus DNA gyrase, 0.125 and 0.25 microg/mL against E. coli DNA gyrase). On the basis of the biological results, structure-activity relationships are also discussed. 相似文献
6.
Ouyang G Chen Z Cai XJ Song BA Bhadury PS Yang S Jin LH Xue W Hu DY Zeng S 《Bioorganic & medicinal chemistry》2008,16(22):9699-9707
Fourteen title compounds, 1-substituted-5-substitutedphenylthio-4-pyrazolaldoxime ester derivatives 4a-4n, were synthesized from the starting material 1-substitutedphenyl-3-methyl-5-substitutedphenylthio-4-pyrazolaldoximes 3 by treatment with acyl chloride. The synthesized compounds were characterized by physical constants, and the structures of the title compounds were further confirmed by IR, 1H NMR, 13C NMR and elemental analysis. The bioassay results showed that title compounds possessed weak to good anti-TMV bioactivity with 4l showing significant enhancement of disease resistance in tobacco leaves with high affinity for TMV CP. 相似文献
7.
Asymmetric addition of dialkyl phosphites (--CH2CH3, --CH2CH2CH3, --CH(CH3)2, --CH2(CH2)3CH3, --CH2CH2OCH3 and --CH2CH2OC2H5) induced by chiral organocatalyst e.g. (R)- and (S)-3,3'-[3,5-bis(trifluoromethyl)phenyl]2-1,1'-binaphthyl phosphate on fluorinated aldimines derived from cinnamaldehyde has been found effective to give new bioactive alpha-aminophosphonates in good yields (58-73%) and high enantiomeric excess (64.6%-90.6%) under mild conditions. 相似文献
8.
Yuping Zhang Song Bai Baoan Song Pinaki S. Bhadury Deyu Hu Song Yang Xiaoyan Zhang Huitao Fan Ping Lu 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2010,878(17-18):1285-1289
Enantiomers of some new quinazoline derivatives bearing α-aminophosphonate moiety were separated under normal-phase conditions on two immobilized polysaccharide-based chiral stationary phases (Chiralpak IA and Chiralpak IC). The role of two chiral stationary phases (CSPs), polar modifier and column temperature on retention time and separation factor was studied. Apparent thermodynamic parameters were deduced from Van’t Hoff plots and plausible mechanism of chiral recognition has been discussed. The semi-preparative separation of some compounds was executed successfully in n-hexane/isopropyl alcohol (IPA) on the Chiralpak IA column. The preliminary bioassay showed that both the enantiomers of the investigated series of compounds possessed similar anti-tobacco mosaic virus (TMV) activities. 相似文献
9.
Bhadury P Mohammad BT Wright PC 《Journal of industrial microbiology & biotechnology》2006,33(5):325-337
A growing number of marine fungi are the sources of novel and potentially life-saving bioactive secondary metabolites. Here, we have discussed some of these novel antibacterial, antiviral, antiprotozoal compounds isolated from marine-derived fungi and their possible roles in disease eradication. We have also discussed the future commercial exploitation of these compounds for possible drug development using metabolic engineering and post-genomics approaches. 相似文献
10.
Punyasloke Bhadury†¶ Melanie C. Austen David T. Bilton† P. John D. Lambshead‡ Alex D. Rogers§ & Gary R. Smerdon 《Zoologica scripta》2007,36(1):93-98
Museums and other research organizations around the world have large numbers of formalin-fixed marine invertebrates in their collections. These have the potential to be a valuable resource for molecular ecological studies, but the development of methodologies for the molecular analysis of formalin-fixed material has been slow. In this study, a hot lysis protocol accompanied by the use of a commercial DNA extraction kit has been employed for DNA recovery from archived marine nematodes, followed by PCR amplification and sequencing. In total, 25 specimens ranging from estuarine to deep sea environments were subjected to molecular analyses. Successful amplification and sequencing of the nuclear small subunit ribosomal RNA (18S rRNA) gene was achieved in all individuals. Additionally, some estuarine nematodes were tentatively identified to genus and species using a phylogenetic approach. In the future, this technique should prove to be profitable for the genetic study of a wide range of formalin-fixed marine invertebrates. 相似文献