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1.
A A Filippov V V Kutyrev O A Protsenko 《Molekuliarnaia genetika, mikrobiologiia i virusologiia》1987,(10):44-48
The relation of Yersinia pestis calcium dependence plasmid (pCad) to known Inc FI (F'lac, R386, pOX38) and IncFV (F0lac) plasmids has been studied. Evidence that plasmid pCad of Yersinia pestis belongs to FI incompatibility group is presented. 相似文献
2.
Plasmid content in Yersinia pestis strains of different origin 总被引:5,自引:0,他引:5
Andrew A. Filippov Nikolai S. Solodovnikov Liubov M. Kookleva Olga A. Protsenko 《FEMS microbiology letters》1990,67(1-2):45-48
Plasmid content in 242 Yersinia pestis strains from various natural plague foci of the U.S.S.R. and other countries was studied. Of these strains, 172 (71%) were shown to carry three plasmids described previously of about 6, 45-50 and 60 MDa, respectively. Twenty strains (8%) from different foci harboured additional cryptic plasmids, most often of about 20 mDa in size. Plasmid pPst displayed considerable constancy of its molecular mass. On the contrary, size variations of pCad (45-49 MDa) and, especially, pFra (60-190 MDa) were found. Molecular mass of these plasmids correlated with the host strain origin. 相似文献
3.
A A Filippov N A Vidiaeva V V Kutyrev P N Ole?nikov L M Kukleva O A Protsenko 《Genetika》1991,27(4):598-608
The genetic locus of Yersinia pestis encoding synthesis of a 46-kDa heat-inducible outer membrane protein (Omp2) was cloned into pBR322 plasmid. The Omp2 was shown to be analogous to previously described YopH and Yop2b proteins. The fifth HindIII fragment of 48-MDa calcium dependence plasmid pCad358 mediates production of 31- and 28-kDa proteins, irrespective of orientation of the insertion. A 31-kDa polypeptide seems to correspond to the YopJ described elsewhere. The maps of BamHI and HindIII of pCad358 region studied differed from those described for pCD1 plasmid of Y. pestis KIM. The products encoded by genes from the fragment cloned in the Pgm+ background give rise to considerable growth of Y. pestis within mouse peritoneal macrophages but were not sufficient to cause lethal infectious process. 相似文献
4.
The influence of amino acids--glycine, alpha-alanine, lysine, phenylalanine, serine, methionine, cysteine, tryptophan, aspartic acid and asparagine--and proteins--chick egg albumin and autolysate of Aspergillus niger biomass--on the disintegration of beet pectine by the Asp. niger enzymic preparation was investigated. This process was shown to depend on the chemical composition and concentration of amino acids and the protein nature. All the tested amino acids, except tryptophan, at doses of 0.0001 to 0.5% stimulated the activity of pectolytic enzymes. The effect of proteins was different: Asp. niger autolysate showed no essential influence and egg albumin inhibited pectine disintegration. 相似文献
5.
A. G. Kursanov R. V. Lisin S. Yu. Khamzin A. A. Balakin Yu. L. Protsenko O. E. Solovyova 《Biophysics》2016,61(5):759-764
The results of numerical simulations and physiological experiments that assessed the dependence of the intramyocardial slow force response under an afterload in the heterogeneous myocardium are described. Muscle duplexes of our design were used as the simplest experimental and theoretical models of the heterogeneous myocardium. It is shown that the degree of the slow force response increases with an afterload and the time of the stimulation delay between the elements of the duplex. This effect is explainable by an increase in the time of the mechanical interaction between the duplex elements in the isometric phase of contraction. This leads to changes in individual contractility, the configuration of the action potential, and the kinetic characteristics of intracellular calcium in cardiomyocytes of interacting muscles. 相似文献
6.
Buza NL Krinitsyna AA Protsenko MA Vartapetian VV 《Prikladnaia biokhimiia i mikrobiologiia》2004,40(1):104-108
We investigated the dynamics of the activity of the polygalacturonidase inhibitor protein (PGIP) in apple fruits of six varieties differing in ripening time and correlated it with the degree of damage by the causative agent of fruit rot, Monilia fructigena. The apple varieties studied differed significantly in PGIP activity and degree of damage by Monilia fructigena. The rate of dissemination over fruit tissues was inversely related to PGIP activity. The resistance of apples to M. fructigena increased with ripening. The simultaneous increase in PGIP activity suggests its important role in the reduction of apple damage by fruit rot. 相似文献
7.
Chian KhK Thang NT Syong NK Bulantseva EA Protsenko MA Sal'kova EG 《Prikladnaia biokhimiia i mikrobiologiia》2000,36(2):229-232
Effects of Exin on infection of tomato, potato, and cabbage plants with Pseudomonas solanacearum and Erwinia carotovora and a fungus Sclerotium rolfsii were studied. The treatment of infected plants with Exin caused no significant effect on the development of the disease. Treatment with streptomycin as a standard for comparison completely inhibited the growth of these microorganisms. Pretreatment with Exin one to eight days before infecting inhibited the development of diseases. The numbers of tomato and potato plants damaged among those infected with P. solanacearum were lower by 10 and 35% respectively. In field experiments (350 plants per variant), treatment with Exin decreased the development of wilt caused by S. rolfsii and P. solanacearum and rot caused by E. carotovora. Treatment with Exin activated the release of ethylene for not less than 30 days. Possible mechanisms of the effects of Exin are discussed. 相似文献
8.
Bulantseva EA Thang N Buza NL Krinitsyna AA Protsenko MA 《Prikladnaia biokhimiia i mikrobiologiia》2005,41(3):288-291
The activity of polygalacturonase and the protein inhibiting this enzyme, which affected polygalacturonases of phytopathogenic fungi Verticillium dahliae and Gloesporium musarium, were detected in banana (Musa acumthata L.) fruit of cultivars Cavendish and Korolevskii. The polygalacturonase from banana fruit was inhibited by the preparations of the protein inhibitor not only from bananas but also from potato (Solanum tuberosum L.) tubers and pepper (Capsicum annuum L.) fruit. 相似文献
9.
E. A. Bulantseva Nguen T’en Tkhang N. L. Buza A. A. Krinitsyna M. A. Protsenko 《Applied Biochemistry and Microbiology》2005,41(3):251-253
The activity of polygalacturonase and the protein inhibiting this enzyme, which affected polygalacturonases of phytopathogenic fungi Verticillium dahliae and Gloesporium musarum, were detected in banana (Musa acuminata L.) fruit of cultivars Cavendish and Korolevskii. The polygalacturonase from banana fruit was inhibited by the preparations of the protein inhibitor not only from bananas but also from potato (Solanum tuberosum L.) tubers and pepper (Capsicum annuum L.) fruit.__________Translated from Prikladnaya Biokhimiya i Mikrobiologiya, Vol. 41, No. 3, 2005, pp. 288–291.Original Russian Text Copyright © 2005 by Bulantseva, Nguen Ten Tkhang, Buza, Krinitsyna, Protsenko. 相似文献
10.
We present here a 3D mathematical model of rheological properties of a morphofunctional unit of myocardium as example of biological
tissue. The model consists of longitudinal and transverse elastic elements and inclined viscoelastic elements connected pivotally
without friction. The parameters of viscosity and elasticity of the structural elements of the model do not depend on the
magnitude of deformation. The model adequately describes nonlinear viscoelastic behavior of isolated samples of passive myocardium
both in static condition and under dynamic loading. The simulation data fit the experiment very well both for intact rat papillary
muscle and for a decellularized specimen. 相似文献