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1.
Robert Blake II Elizabeth A. Shute James Waskovsky Arthur P. Harrison JR. 《Geomicrobiology journal》2013,30(3-4):173-192
Abstract Microorganisms capable of aerobic respiration on ferrous ions are spread throughout eubacterial and archaebacterial phyla. Phylogenetically distinct organisms were shown to express spectrally distinct redox‐active biomolecules during autotrophic growth on soluble iron. A new iron‐oxidizing eubacterium, designated as strain Funis, was investigated. Strain Funis was judged to be different from other known iron‐oxidizing bacteria on the bases of comparative lipid analyses, 16S rRNA sequence analyses, and cytochrome composition studies. When grown autotrophically on ferrous ions, Funis produced conspicuous levels of a novel acid‐stable, acid‐soluble yellow cytochrome with a distinctive absorbance peak at 579 nm in the reduced state. Stopped‐flow spectrophotometric kinetic studies were conducted on respiratory chain components isolated from cell‐free extracts of Thiobacillus ferrooxidans. Experimental results were consistent with a model where the primary oxidant of ferrous ions is a highly aggregated c‐type cytochrome that then reduces the periplasmic rusticyanin. The Fe(II)‐dependent, cytochrome c‐catalyzed reduction of the rusticyanin possessed three kinetic properties in common with corresponding intact cells that respire on iron: the same anion specificity, a similar dependence of the rate on the concentration of ferrous ions, and similar rates at saturating concentrations of ferrous ions 相似文献
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Ca2+ is an important intracellular messenger affecting many diverse processes. In eukaryotic cells, Ca2+ storage is achieved within specific intracellular organelles, especially the endoplasmic/sarcoplasmic reticulum, in which Ca2+ is buffered by specific proteins known as Ca2+ buffers. Ca2+ buffers are a diverse group of proteins, varying in their affinities and capacities for Ca2+, but they typically also carry out other functions within the cell. The wide range of organelles containing Ca2+ and the evidence supporting cross-talk between these organelles suggest the existence of a dynamic network of organellar Ca2+ signaling, mediated by a variety of organellar Ca2+ buffers. 相似文献
4.
KHALIFAH R. A.; LEWIS L. N.; COGGINS C. W. JR.; RADLICK P. C. 《Journal of experimental botany》1965,16(3):511-517
Evidence for the non-indolic nature of the new citrus auxinis presented on the basis of fluorometric properties, thin-layerchromatography, Ehrlich's colour reaction, paper electrophoresis,and the infra-red spectra determinations. Citrus auxin had alower Rf in TLC than IAA, did not give the typical indole reactionwith Ehrlich's reagent, and behaved differently in electrophoresis.The infra-red spectra also provided preliminary informationconcerning chemical structure. The hypothesis that indolic compoundsconstitute the only natural auxins in higher plants should berevised in view of this evidence that a non-indole auxin existsin higher plants. 相似文献
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Heterais exul, previously known from Brazil and Argentina, was collected from a number of lakes and streams in the Lakes Region of Chile (between Valdivia and Puerto Montt). It was found in a wide variety of habitats: on leaf litter, detritus, algae, rotting wood, emergent vegetation, and clean sandy bottoms. Heterias, Pseudasellus, and Fritzianira are placed in synonymy, but Fritzianira is maintained as a subgenus of Heterias because of its lack of an antenna) scale. The 3 Australian species of Heterias and H. exul are believed to have evolved from a common freshwater Gondwanaland ancestor. 相似文献
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Major histocompatibility (B) complex and sex effects on the phytohaemagglutinin wattle response 总被引:2,自引:0,他引:2
The influence of the major histocompatibility (B) complex and sex on the phytohaemagglutinin (PHA) wattle response was studied in 136 segregants (B2/B2, B2/B5 and B5/B5) of a fourth generation cross between inbred lines 6(1) and 15(1). At 6 weeks of age, chickens were injected with 100 micrograms purified PHA-P. Wattle thickness measurements were taken 4, 24, 48, 72 and 96 h after injection. Analysis of variance showed that 4 h after injection, males had a significantly higher response than females but the sex-genotype interaction was also significant. Females had higher responses than males 24 and 48 h after injection as a consequence of more rapid development and earlier resolution of the reaction in males. B2/B2 chickens had significantly lower responses than B5/B5 chickens 72 and 96 h after injection, signifying a faster late resolution phase in the B2/B2 genotype. The developmental and early resolution phases of the PHA wattle response were influenced by sex while the late resolution phase was influenced by B genotype. 相似文献
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Allometric Root/Shoot Relationships and Predicted Water Uptake for Desert Succulents 总被引:3,自引:0,他引:3
Root morphology, shoot morphology, and water uptake for Agavedeserti and Ferocactus acanthodes of various sizes were studiedusing allometric relationships (y = axb) and a previously developedwater uptake model. Shoot surface area increased with shootvolume with an exponent b of 0.75 for both species. Root lengthand the ground area explored by the roots increased with shootsurface area with b's of 0.72 for A. deserti and 0.92 for F.acanthodes. Various sized individuals had about the same ratioof root length to explored ground area, with higher values occurringfor A. deserti. Predicted water uptake averaged over the exploredground area was approximately constant over a 104-fold rangein shoot surface area, suggesting that shoot size confers nointraspecific competitive advantage for water uptake. For theroot lengths per explored ground area observed in the field,water uptake was predicted to be 85 per cent of maximal; wateruptake could be increased by the production of more rain roots.When differences in shoot volume were accounted for by allometry,small plants had relatively less shoot surface area and relativelymore root length per shoot volume than did large plants, whichmay be important for the water relations of seedling establishment. Agave deserti, Ferocactus acanthodes, allometry, desert succulents, root distribution, root length, seedling growth, seedling establishment, shoot surface area, shoot volume, water uptake 相似文献
8.
Light-Induced Polar pH Changes in Leaves of Elodea canadensis: I. Effects of Carbon Concentration and Light Intensity 总被引:2,自引:2,他引:0 下载免费PDF全文
Leaves of the submerged aquatic Elodea canadensis Michx. exhibit a light induced polar pH reaction. In this study, the effects of light intensity and dissolved inorganic carbon concentration on this polar reaction were examined. At a light intensity of 100 watts per square meter the leaf showed a polar pH response when the dissolved inorganic carbon concentration was less than about 1 millimolar. The polar reaction was suppressed at a higher dissolved inorganic carbon concentration. This suppression was not due to the buffering capacity of bicarbonate. Because another weak acid, acetate, did not inhibit the polarity, but even had a small stimulatory effect, the effect of bicarbonate is also not due to acidification of the cytoplasm. The suppression of the polar reaction by CO2/HCO3− was relieved when the light intensity was increased. Apparently there is competition for product(s) of the photosynthetic light reactions between processes generating the polar reaction and the carbon fixation reactions. The possibility that the redox state of the cell regulates the generation of the polar reaction is discussed. 相似文献
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Thermophiles: A life at elevated temperatures 总被引:1,自引:0,他引:1
Interest in the ecology, physiology and evolution of microorganisms adapted to growth at relatively high temperatures (up to 110°C) has increased enormously during the past two decades. This interest was stimulated by the discovery of marine hydrothermal vent ecosystems, and also by awareness of the potential of thermophilic microbes in biotechnological processes. Subsequent attempts to isolate new thermophilic organisms have been very successful. Moreover, these results, in combination with much-improved techniques for studying the phylogeny of microorganisms, have renewed interest in the evolution of microbes and the early development of life. 相似文献