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Anchalee Tassanakajon Piti Amparyup Kunlaya Somboonwiwat Premruethai Supungul 《Marine biotechnology (New York, N.Y.)》2010,12(5):487-505
Penaeid shrimp aquaculture has been consistently affected worldwide by devastating diseases that cause a severe loss in production.
To fight a variety of harmful microbes in the surrounding environment, particularly at high densities (of which intensive
farming represents an extreme example), shrimps have evolved and use a diverse array of antimicrobial peptides (AMPs) as part
of an important first-line response of the host defense system. Cationic AMPs in penaeid shrimps composed of penaeidins, crustins,
and anti-lipopolysaccharide factors are comprised of multiple classes or isoforms and possess antibacterial and antifungal
activities against different strains of bacteria and fungi. Shrimp AMPs are primarily expressed in circulating hemocytes,
which is the main site of the immune response, and hemocytes expressing AMPs probably migrate to infection sites to fight
against pathogen invasion. Indeed, most AMPs are produced as early as the nauplii developmental stage to protect shrimp larvae
from infections. In this review, we discuss the sequence diversity, expression, gene structure, and antimicrobial activities
of cationic AMPs in penaeid shrimps. The information available on antimicrobial activities indicates that these shrimp AMPs
have potential therapeutic applications in the control of disease problems in aquaculture. 相似文献
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Suthianthong P Pulsook N Supungul P Tassanakajon A Rimphanitchayakit V 《Fish & shellfish immunology》2011,30(3):783-790
A homolog of mammalian secretory leucocyte proteinase inhibitor or SLPI known as a double WAP domain (DWD) protein has been found in penaeid shrimp and believed to play an important role in innate immune system of the shrimp. The PmDWD identified from the Penaeus monodon EST database was investigated for its expression under pathogen infection. Infections by Vibrio harveyi and white spot syndrome virus (WSSV) up-regulated the expression of the PmDWD, which was peaked at about 24 h post infection and, then, subsided to more or less normal level. The PmDWD was expressed in various tissues of normal, 24-h WSSV-injected and leg-amputated shrimp, predominantly in the hemocytes. The expression was dramatically increased in lymphoid organ upon WSSV infection and leg amputation. The recombinant PmDWD (rPmDWD) was not active against the commercial proteinases: trypsin, chymotrypsin, elastase and subtilisin while its mutant rPmDWD_F70R was active against the subtilisin. By using agar diffusion assay, the rPmDWD inhibited the crude proteinases from lymphoid organs of leg-amputated and WSSV-infected shrimp. It inhibited the crude proteinases from Bacillus subtilis as well. Unlike the mammalian SLPIs, the rPmDWD had no antimicrobial activity against various bacteria. 相似文献
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Supungul P Sootanan P Klinbunga S Kamonrat W Jarayabhand P Tassanakajon A 《Marine biotechnology (New York, N.Y.)》2000,2(4):339-347
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Cationic Antimicrobial Peptides in Penaeid Shrimp 总被引:1,自引:0,他引:1
Tassanakajon A Amparyup P Somboonwiwat K Supungul P 《Marine biotechnology (New York, N.Y.)》2011,13(4):639-657
Penaeid shrimp aquaculture has been consistently affected worldwide by devastating diseases that cause a severe loss in production.
To fight a variety of harmful microbes in the surrounding environment, particularly at high densities (of which intensive
farming represents an extreme example), shrimps have evolved and use a diverse array of antimicrobial peptides (AMPs) as part
of an important first-line response of the host defense system. Cationic AMPs in penaeid shrimps composed of penaeidins, crustins,
and anti-lipopolysaccharide factors are comprised of multiple classes or isoforms and possess antibacterial and antifungal
activities against different strains of bacteria and fungi. Shrimp AMPs are primarily expressed in circulating hemocytes,
which is the main site of the immune response, and hemocytes expressing AMPs probably migrate to infection sites to fight
against pathogen invasion. Indeed, most AMPs are produced as early as the nauplii developmental stage to protect shrimp larvae
from infections. In this review, we discuss the sequence diversity, expression, gene structure, and antimicrobial activities
of cationic AMPs in penaeid shrimps. The information available on antimicrobial activities indicates that these shrimp AMPs
have potential therapeutic applications in the control of disease problems in aquaculture. 相似文献
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Identification of immune-related genes in hemocytes of black tiger shrimp (Penaeus monodon) 总被引:3,自引:0,他引:3
Supungul P Klinbunga S Pichyangkura R Jitrapakdee S Hirono I Aoki T Tassanakajon A 《Marine biotechnology (New York, N.Y.)》2002,4(5):487-494
An expressed sequence tag (EST) library was constructed from hemocytes of the black tiger shrimp (Penaeus monodon) to identify genes associated with immunity in this economically important species. The number of complementary DNA clones in the constructed library was approximately 4 x 10(5). Of these, 615 clones having inserts larger than 500 bp were unidirectionally sequenced and analyzed by homology searches against data in GenBank. Significant homology to known genes was found in 314 (51%) of the 615 clones, but the remaining 301 sequences (49%) did not match any sequence in GenBank. Approximately 35% of the matched ESTs were significantly identified by the BLASTN and BLASTX programs, while 65% were recognized only by the BLASTX program. Of the 615 clones, 55 (8.9%) were identified as putative immune-related genes. The isolated genes were composed of those coding for enzymes and proteins in the clotting system and the prophenoloxidase-activating system, antioxidative enzymes, antimicrobial peptides, and serine proteinase inhibitors. Three full-length ESTs encoding antimicrobial peptides (antilipopolysaccharide and penaeidin homologues) and a heat shock protein (cpn10 homologue) are reported. 相似文献
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Anchalee Tassanakajon Kunlaya Somboonwiwat Premruethai Supungul Sureerat Tang 《Fish & shellfish immunology》2013,34(4):954-967
Several immune-related molecules in penaeid shrimps have been discovered, most of these via the analysis of expressed sequence tag libraries, microarray studies and proteomic approaches. These immune molecules include antimicrobial peptides, serine proteinases and inhibitors, phenoloxidases, oxidative enzymes, clottable protein, pattern recognition proteins, lectins, Toll receptors, and other humoral factors that might participate in the innate immune system of shrimps. These molecules have mainly been found in the hemolymph and hemocytes, which are the main sites where immune reactions take place, while some are found in other immune organs/tissues, such as the lymphoid organs, gills and intestines. Although the participation of some of these immune molecules in the shrimp innate immune defense against invading pathogens has been demonstrated, the functions of many molecules remain unclear. This review summarizes the current status of our knowledge concerning the discovery and functional characterization of the immune molecules in penaeid shrimps. 相似文献
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