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The effect of neurotensin on submaximally-stimulated hepatobiliary and pancreatic secretion was studied in 6 healthy subjects. An intravenous infusion of neurotensin 1.4 ± 0.3 pmol/kg/min, designed to reproduce plasma neurotensin immunoreactivity levels within the physiological range, produced a significant increase in pancreatic bicarbonate output. Plasma concentrations of pancreatic polypeptide rose by 83 ± 16 pmol/l and were associated with a small reduction in trypsin, but no significant change in bilirubin outputs. 相似文献
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Eugene R. Zabarovsky Ilya M. Chumakov Vladimir S. Prassolov Lev L. Kisselev 《Gene》1984,30(1-3):107-111
We have determined the nucleotide sequence of an 841-bp fragment derived from a segment of the human genome previously cloned by Chumakov et al. [Gene 17 (1982) 19–26] and Zabarovsky et al. [Gene 23 (1983) 379–384] and containing regions homologous to the viral mos gene probe. This sequence displays homology with part of the coding region of the human and murine c-mos genes, contains several termination codons, and is interrupted by two Alu-family elements flanked by short direct repeats. Probably, the progenitor of the human c-mos gene was duplicated approximately at the time of mammalian divergence, was converted to a pseudogene, and acquired insertions of two Alu elements. 相似文献
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Molecular Biology - The mechanisms involved in the origin and development of malignant and neurodegenerative diseases are an important area of modern biomedicine. A crucial task is to identify new... 相似文献
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Acute myeloid leukemia (AML) is a malignant blood disease caused by different mutations that enhance the pro-liferative activity
and survival of blood cells and affect their differentiation and apoptosis. The most frequent disorders in AML are translocations
between chromosomes 21 and 8 leading to production of a chimeric oncogene, AML1-ETO, and hyperexpression of the receptor tyrosine kinase KIT. Mutations in these genes often occur jointly. The presence in cells
of two activated oncogenes is likely to trigger their malignization. The current approaches for treatment of oncologic diseases
(bone marrow transplantation, radiotherapy, and chemotherapy) have significant shortcomings, and thus many laboratories are
intensively developing new approaches against leukemias. Inhibiting expression of activated leukemic oncogenes based on the
principle of RNA interference seems to be a promising approach in this field. 相似文献
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RNA interference is one of the most important mechanisms regulating gene expression. Small interfering RNAs (siRNAs) play an essential role in cell defense against virus infection or retrotransposons. The use of siRNAs gives wide opportunities for treating virus infections and cancer. RNA interference allows rapid construction of monogenic functional knockouts, thereby providing a convenient tool for researchers. The review considers the current views of the mechanisms of RNA interference and modern approaches to its practical application. 相似文献
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Natalia F. Zakirova Alexander V. Shipitsyn Maxim V. Jasko Maria M. Prokofjeva Valeria L. Andronova Georgiy A. Galegov Vladimir S. Prassolov Sergey N. Kochetkov 《Bioorganic & medicinal chemistry》2012,20(19):5802-5809
The antiviral activity against HIV and HSV and the chemical stability of ACV phosphoramidate derivatives were studied. The phosphoramidates of ACV demonstrated moderate activity. The best compound appeared to be 9-(2-hydroxymethyl)guanine phosphoromonomorpholidate (7), which inhibited virus replication in pseudo-HIV-1 particles by 50% at 50 μM. It also inhibited replication of wild-type HSV-1 (9.7 μM) as well as an acyclovir-resistant strain (25 μM). None of the synthesised compounds showed any cytotoxicity. 相似文献
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Dmitri E. Andreev Sergey E. Dmitriev Ilya M. Terenin Vladimir S. Prassolov William C. Merrick Ivan N. Shatsky 《Nucleic acids research》2009,37(18):6135-6147
Many mammalian mRNAs possess long 5′ UTRs with numerous stem-loop structures. For some of them, the presence of Internal Ribosome Entry Sites (IRESes) was suggested to explain their significant activity, especially when cap-dependent translation is compromised. To test this hypothesis, we have compared the translation initiation efficiencies of some cellular 5′ UTRs reported to have IRES-activity with those lacking IRES-elements in RNA-transfected cells and cell-free systems. Unlike viral IRESes, the tested 5′ UTRs with so-called ‘cellular IRESes’ demonstrate only background activities when placed in the intercistronic position of dicistronic RNAs. In contrast, they are very active in the monocistronic context and the cap is indispensable for their activities. Surprisingly, in cultured cells or cytoplasmic extracts both the level of stimulation with the cap and the overall translation activity do not correlate with the cumulative energy of the secondary structure of the tested 5′ UTRs. The cap positive effect is still observed under profound inhibition of translation with eIF4E-BP1 but its magnitude varies for individual 5′ UTRs irrespective of the cumulative energy of their secondary structures. Thus, it is not mandatory to invoke the IRES hypothesis, at least for some mRNAs, to explain their preferential translation when eIF4E is partially inactivated. 相似文献