排序方式: 共有45条查询结果,搜索用时 15 毫秒
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Understanding the plant immune system is crucial for using genetics to protect crops from diseases. Plants resist pathogens via a two-tiered innate immune detection-and-response system. The first plant Resistance (R) gene was cloned in 1992 . Since then, many cell-surface pattern recognition receptors (PRRs) have been identified, and R genes that encode intracellular nucleotide-binding leucine-rich repeat receptors (NLRs) have been cloned. Here, we provide a list of characterized PRRs and NLRs. In addition to immune receptors, many components of immune signaling networks were discovered over the last 30 years. We review the signaling pathways, physiological responses, and molecular regulation of both PRR- and NLR-mediated immunity. Recent studies have reinforced the importance of interactions between the two immune systems. We provide an overview of interactions between PRR- and NLR-mediated immunity, highlighting challenges and perspectives for future research.A review of major research advances in plant immunity during the last three decades and individual characterized immune receptors, their immune signaling pathways, and interactions between immune systems 相似文献
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Proteins are dynamic molecules that often undergo conformational changes while performing their specific functions, such as target recognition, ligand binding and catalysis. NMR spectroscopy is uniquely suited to study protein dynamics, because site-specific information can be obtained for motions that span a broad range of time scales. The information obtained from NMR dynamics experiments has provided insights into specific structural changes or conformational energetics associated with molecular function. In the last decade, a number of new advancements in NMR methodologies have further extended our ability to characterize protein dynamics. Here, we present an overview of current NMR technology that is used to monitor the dynamic properties of proteins. 相似文献
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Otakar Rop Jiri Mlcek Tunde Jurikova Magdalena Valsikova 《Central European Journal of Biology》2012,7(4):672-679
At present, Cape gooseberry (Physalis peruviana) fruit is one of the less used raw materials of plant origin, which can be used for human nutrition. This fruit, as well
as alimentary products made of it, were used by healers in folk medicine in the distant past. The aim of this study was to
monitor and evaluate the antioxidant capacity of fresh fruit of three Cape gooseberry cultivars ‘Giant’, ‘Golden berry’ and
‘Inka’. Antioxidant capacity was also tested, on the basis of the scavenging effect of reactive oxygen species (ROS) and lipid
peroxidation of methanolic extracts made of fresh fruit. These results were further extended and supplemented with determinates
of the vitamin C and total phenolic contents. These analyses were made for three consecutive years. The highest values of
antioxidant capacity were observed in the ‘Inka’ cultivar (9.31 grams of ascorbic acid equivalents kg−1 of fresh mass). In this cultivar, the obtained results were corroborated also in ROS and the contents of vitamin C and total
phenolics. Due to a high antioxidant capacity of this fruit species, the results presented should increase its popularity
above all as a promising raw material, which can be used for human nutrition. 相似文献
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Chan YW On KF Chan WM Wong W Siu HO Hau PM Poon RY 《The Journal of biological chemistry》2008,283(23):15716-15723
Although cells can exit mitotic block aberrantly by mitotic slippage, they are prevented from becoming tetraploids by a p53-dependent postmitotic checkpoint. Intriguingly, disruption of the spindle-assembly checkpoint also compromises the postmitotic checkpoint. The precise mechanism of the interplay between these two pivotal checkpoints is not known. We found that after prolonged nocodazole exposure, the postmitotic checkpoint was facilitated by p53. We demonstrated that although disruption of the mitotic block by a MAD2-binding protein promoted slippage, it did not influence the activation of p53. Both p53 and its downstream target p21(CIP1/WAF1) were activated at the same rate irrespective of whether the spindle-assembly checkpoint was enforced or not. The accelerated S phase entry, as reflected by the premature accumulation of cyclin E relative to the activation of p21(CIP1/WAF1), is the reason for the uncoupling of the postmitotic checkpoint. In support of this hypothesis, forced premature mitotic exit with a specific CDK1 inhibitor triggered DNA replication without affecting the kinetics of p53 activation. Finally, replication after checkpoint bypass was boosted by elevating the level of cyclin E. These observations indicate that disruption of the spindle-assembly checkpoint does not directly influence p53 activation, but the shortening of the mitotic arrest allows cyclin E-CDK2 to be activated before the accumulation of p21(CIP1/WAF1). These data underscore the critical relationship between the spindle-assembly checkpoint and the postmitotic checkpoint in safeguarding chromosomal stability. 相似文献
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Protein p63 is a key regulator in cell proliferation and cell differentiation in stratified squamous epithelium. ΔNp63α is the most commonly expressed p63 isoform, which is often overexpressed in human tumor. In the present work we report the potential involvement of ΔNp63α in cell cycle regulation. ΔNp63α accumulated in mitotic cells but its expression decreased during mitotic exit. Moreover, ΔNp63α knockdown promoted mitotic exit. ΔNp63α shares a conserved destruction box (D-box) motif with other potential targets of the Anaphase-Promoting Complex/Cyclosome (APC/C). Overexpression of APC/C coactivator Cdh1 destabilized ΔNp63α. Our results suggest that ΔNp63α level is cell cycle-regulated and may play a role in the regulation of mitotic exit. 相似文献
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Jiri Mlcek Otakar Rop Tűnde Jurikova Jiri Sochor Miroslav Fisera Stefan Balla Mojmir Baron Jan Hrabe 《Central European Journal of Biology》2014,9(11):1078-1086
Sweet rowanberries originated by the crossbreeding of wild rowanberries with other fruit species such as apples, medlars or black chokeberries. They are highly resistant to cold climate. In contrast with wild rowanberries, they have sweet mild taste and show less parasorbic acid toxicity, which can be eliminated, when the consumption is excessive, by heating. The objective of the work was to determine selected antioxidant properties in 6 cultivars. The analyses showed that the contents of total phenolics, total flavonoids and ascorbic acid were high. Similarly, antioxidant capacity (6.58–9.62 g of ascorbic acid equivalents kg?1) was also high. The work brings novel data, in particular, when comparing the cultivars; moreover, results regarding reactive oxygen and nitrogen species scavenging activity in sweet rowanberries are being published for the first time. The sweet rowanberry extracts (10%) showed inhibitory ability on hydroxyl radical (16.12–24.73%), superoxide anion (26.74–34.02%), nitric oxide (24.75–31.39%), and lipid peroxidation (7.93–13.12%). The values obtained are even many times higher than those found in common commercial fruit species like apples. Therefore, sweet rowanberries appear to be a promising fruit species for human nutrition, especially due to their high content of bioactive substances and ease of cultivation in worse climatic and soil conditions. 相似文献