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Nieznanski K Podlubnaya ZA Nieznanska H 《Biochemical and biophysical research communications》2006,349(1):391-399
A growing body of evidence points to an association of prion protein (PrP) with microtubular cytoskeleton. Recently, direct binding of PrP to tubulin has also been found. In this work, using standard light scattering measurements, sedimentation experiments, and electron microscopy, we show for the first time the effect of a direct interaction between these proteins on tubulin polymerization. We demonstrate that full-length recombinant PrP induces a rapid increase in the turbidity of tubulin diluted below the critical concentration for microtubule assembly. This effect requires magnesium ions and is weakened by NaCl. Moreover, the PrP-induced light scattering structures of tubulin are cold-stable. In preparations of diluted tubulin incubated with PrP, electron microscopy revealed the presence of approximately 50 nm disc-shaped structures not reported so far. These unique tubulin oligomers may form large aggregates. The effect of PrP is more pronounced under the conditions promoting microtubule formation. In these tubulin samples, PrP induces formation of the above oligomers associated with short protofilaments and sheets of protofilaments into aggregates. Noticeably, this is accompanied by a significant reduction of the number and length of microtubules. Hence, we postulate that prion protein may act as an inhibitor of microtubule assembly by inducing formation of stable tubulin oligomers. 相似文献
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L. G. Marsagishvili M. D. Shpagina Yu. V. Shatalin V. S. Shubina A. A. Naumov M. M. Potselueva Z. A. Podlubnaya 《Biophysics》2006,51(5):705-709
Amyloid oligomers, protofibrils, and fibrils of various amyloidogenic proteins are known to induce cell death. Tetracycline prevents the formation of fibrils of Aβ peptide and other amyloidogenic proteins and decomposes mature fibrils. It was previously shown that sarcomeric cytoskeletal proteins of the titin family (protein X, protein C, and protein H) in vitro form amyloid fibrils and tetracycline decomposes them. In this work, the concentration and time dependence of the survival of polymorphonuclear leukocytes in the presence of protein X amyloid fibrils is demonstrated. It is also shown that the survival rate increases as fibrils are decomposed by tetracycline. The antibiotic itself is found to be nontoxic. The results obtained show that this approach can be used to evaluate the efficiency of drugs that prevent or rectify amyloidoses. 相似文献
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A. G. Bobylev M. D. Shpagina L. G. Bobyleva A. D. Okuneva L. B. Piotrovsky Z. A. Podlubnaya 《Biophysics》2012,57(3):300-304
A comparative estimation of the ability of complexes of fullerene C60 with polyvinylpyrrolidone and fullerene C60 derivatives (the sodium salt of the polycarboxylic derivative of fullerene C60, sodium fullerenolate), has been carried out. The fullerenes destroyed amyloid fibrils of the Aβ(1–42) peptide of the brain and the muscle X-protein. A study of the effect of fullerenes on muscle actin showed that complexes of fullerene C60 with polyvinylpyrrolidone and sodium fullerenolate did not prevent the filament formation of actin, nor did they destroy its filaments in vitro. Conversely, sodium salt of the polycarboxylic derivative of fullerene C60 destroyed actin filaments and prevented their formation. It was concluded that sodium fullerenolate and complexes of fullerene C60 with polyvinylpyrrolidone are the most effective antiamyloid compounds among the fullerenes examined. 相似文献
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Z. A. Podlubnaya I. Ya. Podol’skii M. D. Shpagina L. G. Marsagishvili 《Biophysics》2006,51(5):701-704
The antiamyloidogenic effect of hydrated fullerence C60 HyFn was shown by electron microscopy. It was found that fullerene binds to growing fibrils formed by the [beta]-amyloid peptide Aβ25–35 and thus prevents their further growth and interferes with the formation of new fibrils. Instead of long broad helically twisted ‘ribbons’ formed by Aβ25–35 in the absence of fullerene, short narrow protofibrils form in its presence. These results suggest that fullerenes can be useful in treatment for Alzheimer’s disease. 相似文献
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M B Kalamkarova I E Moskalenko Z A Podlubnaya I G Strankfeld 《Physiologia Bohemoslovaca》1976,25(6):543-550
A study has been made of some structural and enzymatic properties of myosin and its fragments from denervated white muscles of rabbit in the course of atrophy using different methods: UV-luminiscence, flow birefringence, electromicroscopy, viscosimetry and enzymatic measurements. All the studied parameters had a tendency to decrease; at prolonged observation some properties were partially restored. Considerable changes of structural properties of LMM were revealed: the ability of LMM from denervated muscle to form high-ordered structures which is characteristic of LMM from normal muscle decreased considerably. 相似文献
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Electron microscopic study of alpha-actinin. 总被引:18,自引:0,他引:18
Z A Podlubnaya L A Tskhovrebova M M Zaalishtsbvili G A Stefanenko 《Journal of molecular biology》1975,92(2):357-359
Electron microscopic studies of the structure of purified α-actinin alone and in complex with F-actin have determined the molecular shape and size of this protein. α-Actinin molecules represent rods of about 300 Å in length and about 20 Å in diameter. 相似文献
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It has been shown by fluorescence analysis in vitro that the water-soluble sodium salt of the polycarboxylic derivative of
fullerene C60, fullerenol, and complexes of fullerene C60 with polyvinylpyrrolidone (mol. wt. 25000 and 10000) destroy amyloid fibrils of the brain peptide Aβ(1–42) and prevent their
formation. The results of fluorescence analysis confirmed the data obtained earlier by high-resolution electron microscopy.
Fluorescence analysis and electron microscopy are complementary methods for the selection of effective antiamyloid drugs. 相似文献
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S. S. Popova I. M. Vikhlyantsev N. M. Zakharova Z. A. Podlubnaya E. E. Fesenko 《Doklady. Biochemistry and biophysics》2017,472(1):56-59
Seasonal changes in proteolytic activity and content of calpains in striated muscles of the longtailed ground squirrel Spermophilus undulatus were studied by casein zymography and Western blotting analysis. The results testify to hyperactivation of calpain proteases in the skeletal muscles of awakened animals during the “winter” activity. The observed changes are discussed in the context of adaptation of skeletal muscles of long-tailed ground squirrels to hibernation. 相似文献