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1.
Taras Antal Ivan Konyukhov Alena Volgusheva Tatyana Plyusnina Sergei Khruschev Galina Kukarskikh Sergey Goryachev Andrey Rubin 《Physiologia plantarum》2019,165(3):476-486
The development of high‐performance photobioreactors equipped with automatic systems for non‐invasive real‐time monitoring of cultivation conditions and photosynthetic parameters is a challenge in algae biotechnology. Therefore, we developed a chlorophyll (Chl) fluorescence measuring system for the online recording of the light‐induced fluorescence rise and the dark relaxation of the flash‐induced fluorescence yield (Qa? ? re‐oxidation kinetics) in photobioreactors. This system provides automatic measurements in a broad range of Chl concentrations at high frequency of gas‐tight sampling, and advanced data analysis. The performance of this new technique was tested on the green microalgae Chlamydomonas reinhardtii subjected to a sulfur deficiency stress and to long‐term dark anaerobic conditions. More than thousand fluorescence kinetic curves were recorded and analyzed during aerobic and anaerobic stages of incubation. Lifetime and amplitude values of kinetic components were determined, and their dynamics plotted on heatmaps. Out of these data, stress‐sensitive kinetic parameters were specified. This implemented apparatus can therefore be useful for the continuous real‐time monitoring of algal photosynthesis in photobioreactors. 相似文献
2.
Frank W. Albert Mehmet Somel Miguel Carneiro Ayinuer Aximu-Petri Michel Halbwax Olaf Thalmann Jose A. Blanco-Aguiar Irina Z. Plyusnina Lyudmila Trut Rafael Villafuerte Nuno Ferrand Sylvia Kaiser Per Jensen Svante P??bo 《PLoS genetics》2012,8(9)
Domestication has led to similar changes in morphology and behavior in several animal species, raising the question whether similarities between different domestication events also exist at the molecular level. We used mRNA sequencing to analyze genome-wide gene expression patterns in brain frontal cortex in three pairs of domesticated and wild species (dogs and wolves, pigs and wild boars, and domesticated and wild rabbits). We compared the expression differences with those between domesticated guinea pigs and a distant wild relative (Cavia aperea) as well as between two lines of rats selected for tameness or aggression towards humans. There were few gene expression differences between domesticated and wild dogs, pigs, and rabbits (30–75 genes (less than 1%) of expressed genes were differentially expressed), while guinea pigs and C. aperea differed more strongly. Almost no overlap was found between the genes with differential expression in the different domestication events. In addition, joint analyses of all domesticated and wild samples provided only suggestive evidence for the existence of a small group of genes that changed their expression in a similar fashion in different domesticated species. The most extreme of these shared expression changes include up-regulation in domesticates of SOX6 and PROM1, two modulators of brain development. There was almost no overlap between gene expression in domesticated animals and the tame and aggressive rats. However, two of the genes with the strongest expression differences between the rats (DLL3 and DHDH) were located in a genomic region associated with tameness and aggression, suggesting a role in influencing tameness. In summary, the majority of brain gene expression changes in domesticated animals are specific to the given domestication event, suggesting that the causative variants of behavioral domestication traits may likewise be different. 相似文献
3.
Isolation and Characterization of a Hantavirus from Lemmus sibiricus: Evidence for Host Switch during Hantavirus Evolution 总被引:9,自引:0,他引:9 下载免费PDF全文
Olli Vapalahti ke Lundkvist Vadim Fedorov Christopher J. Conroy Sirpa Hirvonen Angelina Plyusnina Kirill Nemirov Karl Fredga Joseph A. Cook Jukka Niemimaa Asko Kaikusalo Heikki Henttonen Antti Vaheri Alexander Plyusnin 《Journal of virology》1999,73(7):5586-5592
A novel hantavirus, first detected in Siberian lemmings (Lemmus sibiricus) collected near the Topografov River in the Taymyr Peninsula, Siberia (A. Plyusnin et al., Lancet 347:1835-1836, 1996), was isolated in Vero E6 cells and in laboratory-bred Norwegian lemmings (Lemmus lemmus). The virus, named Topografov virus (TOP), was most closely related to Khabarovsk virus (KBR) and Puumala viruses (PUU). In a cross focus reduction neutralization test, anti-TOP Lemmus antisera showed titers at least fourfold higher with TOP than with other hantaviruses; however, a rabbit anti-KBR antiserum neutralized TOP and KBR at the same titer. The TOP M segment showed 77% nucleotide and 88% amino acid identity with KBR and 76% nucleotide and 82% amino acid identity with PUU. However, the homology between TOP and the KBR S segment was disproportionately higher: 88% at the nucleotide level and 96% at the amino acid level. The 3' noncoding regions of KBR and the TOP S and M segments were alignable except for 113- and 58-nucleotide deletions in KBR. The phylogenetic relationships of TOP, KBR, and PUU and their respective rodent carriers suggest that an exceptional host switch took place during the evolution of these viruses; while TOP and KBR are monophyletic, the respective rodent host species are only distantly related. 相似文献
4.
Albert FW Shchepina O Winter C Römpler H Teupser D Palme R Ceglarek U Kratzsch J Sohr R Trut LN Thiery J Morgenstern R Plyusnina IZ Schöneberg T Pääbo S 《Hormones and behavior》2008,53(3):413-421
To better understand the biology of tameness, i.e. tolerance of human presence and handling, we analyzed two lines of wild-derived rats (Rattus norvegicus) artificially selected for tameness and defensive aggression towards humans. In response to a gloved human hand, tame rats tolerated handling, whereas aggressive rats attacked. Cross-fostering showed that these behavioral differences are not caused by postnatal maternal effects. Tame rats were more active and explorative and exhibited fewer anxiety-related behaviors. They also had smaller adrenal glands, larger spleens and lower levels of serum corticosterone. Blood glucose levels were lower in tame rats, whereas the concentrations of nine amino acids were higher. In the brain, tame rats had lower serotonin and higher taurine levels than aggressive rats. Our findings reinforce the notion that tameness is correlated with differences in stress response and will facilitate future efforts to uncover the genetic basis for animal tameness. 相似文献
5.
Simona E Budulac Dirkje S Postma Pieter S Hiemstra Lisette IZ Kunz Mateusz Siedlinski Henriette A Smit Judith M Vonk Bea Rutgers Wim Timens H Marike Boezen the Groningen Leiden Universities Corticosteroids in Obstructive Lung Disease study group 《Respiratory research》2010,11(1):60
Background
Multidrug resistance-associated protein-1 (MRP1) protects against oxidative stress and toxic compounds generated by cigarette smoking, which is the main risk factor for chronic obstructive pulmonary disease (COPD). We have previously shown that single nucleotide polymorphisms (SNPs) in MRP1 significantly associate with level of FEV1 in two independent population based cohorts. The aim of our study was to assess the associations of MRP1 SNPs with FEV1 level, MRP1 protein levels and inflammatory markers in bronchial biopsies and sputum of COPD patients.Methods
Five SNPs (rs212093, rs4148382, rs504348, rs4781699, rs35621) in MRP1 were genotyped in 110 COPD patients. The effects of MRP1 SNPs were analyzed using linear regression models.Results
One SNP, rs212093 was significantly associated with a higher FEV1 level and less airway wall inflammation. Another SNP, rs4148382 was significantly associated with a lower FEV1 level, higher number of inflammatory cells in induced sputum and with a higher MRP1 protein level in bronchial biopsies.Conclusions
This is the first study linking MRP1 SNPs with lung function and inflammatory markers in COPD patients, suggesting a role of MRP1 SNPs in the severity of COPD in addition to their association with MRP1 protein level in bronchial biopsies. 相似文献6.
7.
The involvement of glucocorticoid hormones in the appearance of white spots during embryogenesis in domesticated gray rats was studied. It was shown that in fully pigmented gray rats, prenatal stress and exposure to dexamethasone on days 12–14 of gestation elicited a delay in the migration and development of melanoblasts in embryos. This led to a fourfold increase in the number of offspring with depigmentation on the ventral side of the body. It was also demonstrated that in adult offspring of mothers treated with dexamethasone, the response of the hypothalamo-pituitary-adrenal axis to the emotional stress was lower compared to the controls. The role of glucocorticoids in the appearance of coat depigmentation under animal domestication is discussed. 相似文献
8.
N. N. Barykina T. A. Alekhina V. F. Chuguy O. I. Petrenko I. Z. Plyusnina V. G. Kolpakov 《Russian Journal of Genetics》2004,40(5):485-490
Breeding of GC rats for the predisposition to cataleptic freezing has increased not only the frequency, intensity, and duration of freezing, but also the proportion of irritable or nervous rats with enhanced anxiety, defensive behavior with vocalization, jerky running, and jumpiness. An increased amplitude of the startle reflex is a correlate of this nervousness. The results of the comparison of some behavioral characters in the nervous and freezing GC rats, as well as in S1 and S2 offspring from homogeneous crosses between nervous and freezing GC rats suggest that cataleptic freezing and nervousness are two poles of the same bipolar catatonic reaction. They have a common mechanism, with the alternative or preferential expression of one particular form of the reaction is determined by the external and internal environments or the set of modifier genes in the given individual. 相似文献
9.
I. N. Oskina I. Z. Plyusnina A. Yu. Sysoletina 《Journal of Evolutionary Biochemistry and Physiology》2000,36(2):161-169
The activity of the pituitary-adrenal system was studied in the Norway rats selected for both maintenance and elimination
of the aggressive behavior towards man in the postnatal ontogeny. The selection of the wild rats for domesticated behavior
was shown to result in changes of the pituitary-adrenal system formation in the postnatal ontogeny, which is indicated by
differences in the dynamics of the adrenals weight as well as of corticosterone content in the blood plasma and in the adrenals
both at rest and under stress conditions between the domesticated and aggressive rats. 相似文献
10.
Albert FW Hodges E Jensen JD Besnier F Xuan Z Rooks M Bhattacharjee A Brizuela L Good JM Green RE Burbano HA Plyusnina IZ Trut L Andersson L Schöneberg T Carlborg O Hannon GJ Pääbo S 《Heredity》2011,107(3):205-214
The identification of the causative genetic variants in quantitative trait loci (QTL) influencing phenotypic traits is challenging, especially in crosses between outbred strains. We have previously identified several QTL influencing tameness and aggression in a cross between two lines of wild-derived, outbred rats (Rattus norvegicus) selected for their behavior towards humans. Here, we use targeted sequence capture and massively parallel sequencing of all genes in the strongest QTL in the founder animals of the cross. We identify many novel sequence variants, several of which are potentially functionally relevant. The QTL contains several regions where either the tame or the aggressive founders contain no sequence variation, and two regions where alternative haplotypes are fixed between the founders. A re-analysis of the QTL signal showed that the causative site is likely to be fixed among the tame founder animals, but that several causative alleles may segregate among the aggressive founder animals. Using a formal test for the detection of positive selection, we find 10 putative positively selected regions, some of which are close to genes known to influence behavior. Together, these results show that the QTL is probably not caused by a single selected site, but may instead represent the joint effects of several sites that were targets of polygenic selection. 相似文献