Water-soluble galactomannan from the seeds of Lotus corniculatus L.: structure and properties

Galactomannan, a water-soluble heteropolysaccharide, was isolated from the seed of Far-Eastern population of ground honeysuckle Lotus corniculatus L. (yield, 1.65%). Analysis of this galactomannan showed that is consists of D-mannose and D-galactose residues (molar ratio, 1.22:1). Its aqueous solutions were characterized by specific rotation [alpha]D = +84.10 and characteristic viscosity [eta] = 559 ml/g. Analysis of this heteropolysaccharide using chemical and enzymatic procedures, as well as IR- and 13C-NMR spectroscopy, showed that its main chain comprises 1,4-beta-D-mannopyranose residues, 95.5% of which are substituted at C-6 with single residues of alpha-D-galactopyranose.     

Water-Soluble Galactomannan from the Seed of Ground Honeysuckle (Lotus corniculatus L.): Structure and Properties

Galactomannan, a water-soluble heteropolysaccharide, was isolated from the seed of a Far Eastern population of the ground honeysuckle Lotus corniculatus L. (yield, 1.65%). Analysis of this galactomannan showed that it consists of D-mannose and D-galactose residues (molar ratio, 1.22 : 1). Its aqueous solutions were characterized by a specific rotation []_D= +84.1° and intrinsic viscosity [] = 559 ml/g. Analysis of this heteropolysaccharide using chemical and enzymatic procedures, as well as IR and ¹³C NMR spectroscopy, showed that its main chain comprises 1,4--D-mannopyranose residues, 95.5% of which are substituted at C-6 with single residues of -D-galactopyranose.