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Triin Kollist Heino Moldau Bahtijor Rasulov Vello Oja Heikko Rämma Katja Hüve Pinja Jaspers Jaakko Kangasjärvi Hannes Kollist 《Physiologia plantarum》2007,129(4):796-803
To follow stomatal responses to ozone (O3 ) in different Arabidopsis lines, we constructed a rapid-response O3 exposure/gas-exchange measurement device consisting of eight through-flow whole-rosette cuvettes. To separate rosette from roots and growth substrate, plant is grown through an agar-filled hole in a polished glass plate fixed on the pot. Following insertion of the plant, the plate forms air-tight bottom surface of the cuvette; thus the rosette is enclosed without touching it during any phase of the insertion of the plant to the cuvette. The device allows monitoring rapid responses in the stomatal function. For example, an acute exposure to 150 ppb O3 decreased stomatal conductance to 60–70% of its initial value within 9–12 min. Thereafter, the conductance regained its preexposure value within further 30–40 min in spite of the continuing O3 exposure. The transient decrease was absent in the abscisic acid-insensitive mutant abi2 defective in a class 2C protein phosphatase. This provides an in vivo confirmation that the early transient decrease in stomatal conductance is not a result of physical damage by the reactive oxygen species (ROS) formed from O3 breakdown but reflects the biological action of ROS, transduced through a signalling cascade. Thus, the apparatus will be helpful in specifying complex molecular and genetic interactions in rapid responses in guard cells in vivo. 相似文献
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Many anemophilous, early‐flowering tree genera include allergy plants of world‐wide significance. We studied the synchronisation of high and low pollen years in the genera Betula, Alnus, Corylus, Salix and Populus and the cumulative effects that an increasing number of taxa has on the number of days of exposure to different levels of allergenic pollen in North Europe. The proximal causes of the inter‐annual variations of airborne pollen loads were analysed with a multiple regression analysis. The annual fluctuations of airborne pollen sums were compared between genera and found to be positively correlated among all combinations of genera at the three study sites. Most correlations were statistically significant (p<0.05). The comparison between Betula and Alnus is discussed first. Betula pollen was clearly the most abundant airborne pollen type. The presence of Alnus pollen, however, significantly increased the predisposal to allergenic pollen. At all sites, the number of days per year when the Betula and Alnus pollen counts together exceeded 10 and 100?grains m?3 of air, was found to be greater than the number of days when the Betula pollen counts alone exceeded 10 and 100?m?3 of air. The difference was statistically significant. In Kuopio, the difference was found to be statistically significant even for grains per 1?000?m?3 of air of Betula and Alnus together compared with the same count of Betula pollen alone. Betula, Alnus and Corylus belong to the order Fagales and have cross‐reacting main allergens. The flowering of Alder and Corylus culminate at the same time, two to four weeks earlier than that of Betula. Due to synchronization of high and low years and the mostly non‐overlapping flowering seasons, the time of exposure to pollen may be very long during the high years. Furthermore, Alnus and Corylus pollen may prime allergic people before the onset of the Betula season. 相似文献
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Arabidopsis RADICAL-INDUCED CELL DEATH1 belongs to the WWE protein-protein interaction domain protein family and modulates abscisic acid, ethylene, and methyl jasmonate responses 总被引:1,自引:0,他引:1
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Ahlfors R Lång S Overmyer K Jaspers P Brosché M Tauriainen A Kollist H Tuominen H Belles-Boix E Piippo M Inzé D Palva ET Kangasjärvi J 《The Plant cell》2004,16(7):1925-1937
Experiments with several Arabidopsis thaliana mutants have revealed a web of interactions between hormonal signaling. Here, we show that the Arabidopsis mutant radical-induced cell death1 (rcd1), although hypersensitive to apoplastic superoxide and ozone, is more resistant to chloroplastic superoxide formation, exhibits reduced sensitivity to abscisic acid, ethylene, and methyl jasmonate, and has altered expression of several hormonally regulated genes. Furthermore, rcd1 has higher stomatal conductance than the wild type. The rcd1-1 mutation was mapped to the gene At1g32230 where it disrupts an intron splice site resulting in a truncated protein. RCD1 belongs to the (ADP-ribosyl)transferase domain-containing subfamily of the WWE protein-protein interaction domain protein family. The results suggest that RCD1 could act as an integrative node in hormonal signaling and in the regulation of several stress-responsive genes. 相似文献
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Toropainen E Hornof M Kaarniranta K Johansson P Urtti A 《The journal of gene medicine》2007,9(3):208-216
BACKGROUND: The first objective of the study was to evaluate the transfection of corneal epithelium with non-viral vectors to secrete transgene products into the tear fluid and aqueous humor. The second goal was to evaluate the differentiated corneal epithelial cell culture for transfection studies. METHODS: The human corneal epithelial (HCE) cell line was cultured to different stages of differentiation and transfected with complexes of pCMV-SEAP2 with DOTAP/DOPE, DOTAP/DOPE/protamine sulfate (PS) and polyethylenimine (PEI). The complexes of DOTAP/DOPE with plasmid (CMV-SEAP2 or pCMV-Luc4) were subsequently applied topically to the rabbit eyes. Secreted alkaline phosphatase (SEAP) was analyzed using chemiluminescent assay. Luciferase (Luc) was detected at the mRNA level in cornea and conjunctiva using a qRT-PCR. RESULTS: The transfection levels decreased with differentiation of HCE cells. PEI was effective in transfecting both the dividing and partly differentiated cells, but ineffective in differentiated cells. DOTAP/DOPE showed high activity in differentiated cell cultures, while added PS did not improve transfection. Significant SEAP expression was observed for three days after in vivo transfection in the tear fluid and aqueous humor. The luciferase mRNA was found both in the cornea and conjunctiva. The rates of SEAP secretion from both the basolateral side of differentiated HCE cells and cornea in vivo were within the same range. CONCLUSIONS: Corneal epithelium can be transfected topically to secrete gene products to the tear fluid and aqueous humor. The differentiated HCE model is a useful tool in the evaluation of non-viral carriers for corneal transfection. 相似文献
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Pinja Ilmarinen-Salo Eeva Moilanen Vuokko L Kinnula Hannu Kankaanranta 《Respiratory research》2012,13(1):1-11