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1.
Cicconi R Delpino A Piselli P Castelli M Vismara D 《Molecular and cellular biochemistry》2004,259(1-2):1-7
In Daudi cells, a fraction of the 60 kDa heat shock protein (Hsp60), which is typically a mitochondrial protein, is located on cell membrane. This was demonstrated by the recovery of biotinylated Hsp60 in the anti-Hsp60 immunoprecipitate obtained from cells in which surface-exposed proteins were selectively labeled with biotin. In further experiments, isolated membrane proteins (obtained by two different biochemical methods) were probed in Western blot with two antibodies (N-20 and K-19) directed against different epitopes located, respectively, at the amino- and at the carboxyl-terminus of the Hsp60. Both these antibodies recognized, among the isolated membrane proteins, a unique band with an electrophoretic mobility identical to that of the cytoplasmic Hsp60, thus demonstrating that Hsp60 is present on cell surface as an intact, full-length, protein. FACS analysis, performed with the same two highly specific anti-Hsp60 antibodies, confirmed that both the N-terminus and the C-terminus of the Hsp60 are exposed outside the cell and are accessible for recognition by the corresponding antibody. Moreover, quantitative analysis of the data showed that constitutive cell surface expression of the Hsp60 is limited to a small fraction (about 10%) of the whole cell population. 相似文献
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R. H. Vreeland A. F. Piselli Jr. S. McDonnough S. S. Meyers 《Extremophiles : life under extreme conditions》1998,2(3):321-331
The Waste Isolation Pilot Plant (WIPP) is a salt mine constructed 650 meters below the ground surface by the United States
Department of Energy. The facility will be used for permanent disposal of transuranic wastes. This underground repository
has been constructed in the geologically stable Permian age Salado salt formation. Of the wastes to be placed into the facility,
85% will be biodegradable cellulose. A 3-year survey of the bacterial populations existing within the facility was conducted.
Bacterial populations were found to be heterogeneously distributed throughout the mine. Populations in some mine areas reached
as high as 1.0 × 104 colony-forming units per gram of NaCl. The heterogeneous distribution of bacteria within the mine did not follow any recognizable
pattern related to either age of the workings or to human activity. A biochemical comparison between ten known species of
halophilic bacteria, and strains isolated from both the mine and nearby surface hypersaline lakes, showed the presence of
extreme halophiles with wide biochemical diversity, some of which could prove to represent previously undescribed groups.
The halophilic bacteria isolated from the mine were found to degrade cellulose and a wide variety of other carbon compounds.
When exposed to two types of common laboratory paper, the cellulose-degrading halophiles attached to the substrate within
30 minutes of inoculation. Cultures enriched directly from a brine seep in the mine easily destroyed both papers and produced
detectable amounts of oxalacetic and pyruvic acids. The combination of heterogeneity in the distribution of organisms, the
presence of a physiologically diverse community, and the relatively slow metabolism of cellulose may explain several long-standing
debates about the existence of microorganisms in ancient underground salt formations.
Received: September 29, 1997 / Accepted: January 29, 1998 相似文献
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Riccardo A. A. Muzzarelli Pierluca Ilari Wenshui Xia Marcos Pinotti Marco Tomasetti 《Carbohydrate polymers》1994,24(4):295-300
Stable and self-sustaining gels were obtained from tyrosine glucan (a modified chitosan synthesized with 4-hydroxyphenylpyruvic acid) in the presence of tyrosinase. Similar gels were obtained from 3-hydroxybenzaldehyde, 4-hydroxybenzaldehyde and 3,4-dihydroxybenzaldehyde: all of them were hydrolyzed by lysozyme, lipase and papain. Microcapsules were similarly obtained by introducing tyrosinase in a water-in-oil emulsion containing tyrosine glucan in the water phase. No cross-linking was observed for chitosan derivatives of vanillin, syringaldehyde and salicylaldehyde. Collagen-chitosan-tannin mixtures were also studied under the catalytic action of tyrosinase: partially crystalline, hard, mechanically resistant and scarcely wettable materials were obtained upon drying. By contrast, products obtained from albumin, pseudocollagen and gelatin, in the presence of a number of phenols and chitosan under comparable conditions, were brittle. 相似文献
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Andrea Delpino Anna Maria Mileo Vincenza Lapenta Pierluca Piselli Alessandra Verdina Lorenzo Polenzani 《Journal of cellular biochemistry》1996,63(1):51-60
M-14 human melanoma cells, following severe hyperthermic exposures, synthesized a heat-shock protein of 66 kDa (hsp 66), in addition to the major “classic” heat-shock proteins. This hsp 66 was not expressed following mild hyperthermic exposures sufficient to trigger the synthesis of the other heat-shock proteins. The induction of hsp 66 was observed also in Li human glioma cells treated at 45°C for 20 min. By contrast, hsp 66 was not induced in seven other human cell lines (both melanoma and nonmelanoma) when they were subjected to the same hyperthermic treatment. Immunological recognition experiments showed that hsp 66 cross-reacted with the inducible hsp 72, but not with the constitutive hsp 73. The possibility that hsp 66 is a breakdown product of hsp 72 was ruled out by the fact that Poly(A)+ RNA extracted from cells treated at 45°C for 20 min was able to direct the synthesis of hsp 66 (together with hsp 72) in a message-dependent rabbit reticulocyte lysate, as well as in microinjected Xenopus oocytes. By contrast, only the hsp 72 was expressed using Poly(A)+ RNA extracted from cells heated at 42°C for 1 h. Affinity chromatography experiments on ATP-agarose showed that hsp 66 did not bind ATP in vitro, hsp 66 was localized both in the cytoplasm (cytosol, mitochondria, and microsome fraction) and in the nuclei of cells recovered from a severe heat shock: this intracellular distribution closely corresponded to that of hsp 72. The nuclear-associated hsp 66 was found to be tightly bound to nuclear structures and could not be extracted by incubation in ATP-containing buffer. © 1996 Wiley-Liss, Inc. 相似文献
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Pierluca Coiro Luminita Stoenica Ulf Strauss Anja Ursula Br?uer 《The Journal of biological chemistry》2014,289(36):24956-24970
The transmembrane protein plasticity-related genes 3 and 5 (PRG3 and PRG5) increase filopodial formation in various cell lines, independently of Cdc42. However, information on the effects of PRG5 during neuronal development is sparse. Here, we present several lines of evidence for the involvement of PRG5 in the genesis and stabilization of dendritic spines. First, PRG5 was strongly expressed during mouse brain development from embryonic day 14 (E14), peaked around the time of birth, and remained stable at least until early adult stages (i.e. P30). Second, on a subcellular level, PRG5 expression shifted from an equal distribution along all neurites toward accumulation only along dendrites during hippocampal development in vitro. Third, overexpression of PRG5 in immature hippocampal neurons induced formation of spine-like structures ahead of time. Proper amino acid sequences in the extracellular domains (D1 to D3) of PRG5 were a prerequisite for trafficking and induction of spine-like structures, as shown by mutation analysis. Fourth, at stages when spines are present, knockdown of PRG5 reduced the number but not the length of protrusions. This was accompanied by a decrease in the number of excitatory synapses and, consequently, by a reduction of miniature excitatory postsynaptic current frequencies, although miniature excitatory postsynaptic current amplitudes remained similar. In turn, overexpressing PRG5 in mature neurons not only increased Homer-positive spine numbers but also augmented spine head diameters. Mechanistically, PRG5 interacts with phosphorylated phosphatidylinositols, phospholipids involved in dendritic spine formation by different lipid-protein assays. Taken together, our data propose that PRG5 promotes spine formation. 相似文献
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D Serraino P Piselli P Scognamiglio 《Journal of biological regulators and homeostatic agents》2001,15(3):224-228
Viral infections represent one of the areas in which cancer research has made the greatest advances in the last 20 years. In 1981, only two viruses were known to cause human cancer, i.e., the Epstein-Barr virus (EBV) and the hepatitis B virus (HBV). By 1995, it was estimated that approximately 15% of all cancers occurring world-wide were attributable to viral infections, and the oncogenic role of seven viruses [i.e., EBV, HBV, hepatitis C virus (HCV), human papillomavirus (HPV), human immunodeficiency virus (HIV), human herpesvirus-8 (HHV-8), and human T cell leukemia virus type I (HTLV-I)] has been well-established. In this paper, the epidemiological evidence concerning some of the major aspects of the association between viruses and cancer are summarised. 相似文献
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Barbara Arnò Andrea Coletta Cinzia Tesauro Laura Zuccaro Paola Fiorani Sara Lentini Pierluca Galloni Valeria Conte Barbara Floris Alessandro Desideri 《Bioscience reports》2013,33(2)
The different steps of the human Top1 (topoisomerase I) catalytic cycle have been analysed in the presence of a pentacyclic-diquinoid synthetic compound. The experiments indicate that it efficiently inhibits the cleavage step of the enzyme reaction, fitting well into the catalytic site. Surprisingly the compound, when incubated with the binary topoisomerase–DNA cleaved complex, helps the enzyme to remove itself from the cleaved DNA and close the DNA gap, increasing the religation rate. The compound also induces the religation of the stalled enzyme–CPT (camptothecin)–DNA ternary complex. Analysis of the molecule docked over the binary complex, together with its chemical properties, suggests that the religation enhancement is due to the presence on the compound of two oxygen atoms that act as hydrogen acceptors. This property facilitates the deprotonation of the 5′ DNA end, suggesting that this is the limiting step in the topoisomerase religation mechanism. 相似文献
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Lanini S Garbuglia AR Puro V Solmone M Martini L Arcese W Nanni Costa A Borgia P Piselli P Capobionchi MR Ippolito G 《PloS one》2012,7(3):e33122