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Kyle Denton Morgan M. Atkinson Stacey P. Borenstein Alexis Carlson Thomas Carroll Kristen Cullity Casey DeMarsico Daniel Ellowitz Andrea Gialtouridis Rachel Gore April Herleikson Albee Yun Ling Rachael Martin Katherine McMahan Piangfan Naksukpaiboon Audrey Seiz Katrina Yearwood James O’Neill Heather Wiatrowski 《Archives of microbiology》2013,195(9):661-670
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Gustav N Sundell Roland Arnold Muhammad Ali Piangfan Naksukpaiboon Julien Orts Peter Güntert Celestine N Chi Ylva Ivarsson 《Molecular systems biology》2018,14(8)
A key function of reversible protein phosphorylation is to regulate protein–protein interactions, many of which involve short linear motifs (3–12 amino acids). Motif‐based interactions are difficult to capture because of their often low‐to‐moderate affinities. Here, we describe phosphomimetic proteomic peptide‐phage display, a powerful method for simultaneously finding motif‐based interaction and pinpointing phosphorylation switches. We computationally designed an oligonucleotide library encoding human C‐terminal peptides containing known or predicted Ser/Thr phosphosites and phosphomimetic variants thereof. We incorporated these oligonucleotides into a phage library and screened the PDZ (PSD‐95/Dlg/ZO‐1) domains of Scribble and DLG1 for interactions potentially enabled or disabled by ligand phosphorylation. We identified known and novel binders and characterized selected interactions through microscale thermophoresis, isothermal titration calorimetry, and NMR. We uncover site‐specific phospho‐regulation of PDZ domain interactions, provide a structural framework for how PDZ domains accomplish phosphopeptide binding, and discuss ligand phosphorylation as a switching mechanism of PDZ domain interactions. The approach is readily scalable and can be used to explore the potential phospho‐regulation of motif‐based interactions on a large scale. 相似文献
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